Supramolecular spectrally encoded microgels with double strand probes for absolute and direct miRNA fluorescence detection at high sensitivity [PDF]
, 2015 We present novel microgels as a particle-based suspension array for direct and absolute microRNA (miRNA) detection. The microgels feature a flexible molecular architecture, antifouling properties, and enhanced sensitivity with a large dynamic range of ...
ALIBERTI, ANNA +4 more
core +2 more sources
A paperfluidic platform to detect Neisseria gonorrhoeae in clinical samples [PDF]
, 2018 Globally, the microbe Neisseria gonorrhoeae (NG) causes 106 million newly documented sexually transmitted infections each year. Once appropriately diagnosed, NG infections can be readily treated with antibiotics, but high-risk patients often do not ...
Cabodi, Mario +7 more
core +1 more source
qRT-PCR Platforms for Diagnosing and Reporting SARS-CoV-2 Infection in Human Samples
STAR Protocols, 2020 Summary: The protocols herein outline the use of qRT-PCR to detect the presence of SARS-CoV-2 genomic RNA in patient samples. In order to cope with potential fluctuations in supply chain and testing demands and to enable expedient adaptation of reagents ...
Richard M. Giadone +10 more
doaj +1 more source
Evaluation of a viral microarray based on simultaneous extraction and amplification of viral nucleotide acid for detecting human herpesviruses and enteroviruses. [PDF]
PLoS ONE, 2015 In this study, a viral microarray based assay was developed to detect the human herpesviruses and enteroviruses associated with central nervous system infections, including herpes simplex virus type 1, type 2 (HSV1 and HSV2), Epstein-Barr virus (EBV ...
Yi Liu +7 more
doaj +1 more source
Review Updated Values for Molecular Diagnosis for Highly Pathogenic Avian Influenza Virus [PDF]
, 2012 viruse
Akira Sakurai, Futoshi Shibasaki
core +2 more sources
, 2023 Supplementary Table 1 from Microfluidic-Based Multiplex qRT-PCR Identifies Diagnostic and Prognostic microRNA Signatures in the Sera of Prostate Cancer ...
Robert Blelloch +9 more
openaire +1 more source
The liver X receptor pathway is highly upregulated in rheumatoid arthritis synovial macrophages and potentiates TLR-driven cytokine release [PDF]
, 2013 <p>Objectives: Macrophages are central to the inflammatory processes driving rheumatoid arthritis (RA) synovitis. The molecular pathways that are induced in synovial macrophages and thereby promote RA disease pathology remain poorly understood.</
Ananth +42 more
core +1 more source
Expression and DNA methylation of TNF, IFNG and FOXP3 in colorectal cancer and their prognostic significance. [PDF]
, 2014 BACKGROUND: Colorectal cancer (CRC) progression is associated with suppression of host cell-mediated immunity and local immune escape mechanisms. Our aim was to assess the immune function in terms of expression of TNF, IFNG and FOXP3 in CRC. METHODS:
A D Beggs +52 more
core +2 more sources
Diagnostic Microbiology and Infectious DiseaseHypervirulent Klebsiella pneumoniae (hvKP) pose significant challenges to clinical anti-infective treatment and has emerged as a major threat to global public health. In this study, we employed the loop-mediated isothermal amplification (LAMP) assays with OTG (orange to green) visual dye and multiplex quantitative real-time PCR (qRT-PCR) assay to ...
Yinsheng, Cai +6 more
openaire +2 more sources
Modulation of microRNA editing, expression and processing by ADAR2 deaminase in glioblastoma. [PDF]
, 2015 Background: ADAR enzymes convert adenosines to inosines within double-stranded RNAs, including microRNA (miRNA) precursors, with important consequences on miRNA retargeting and expression.
Alon, S +10 more
core +2 more sources