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Evidence of kinship, overwintering, and Wolbachia presence in Aedes albopictus in urban areas and points of entry in the Netherlands. [PDF]
Ibáñez-Justicia A +13 more
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2021
Amplification of different nucleic acid targets in the same reaction (multiplex polymerase chain reaction) is challenging but an extremely useful tool especially for viroid diagnosis. In the amplification mixtures, several pairs of primers work together in the same conditions to detect different targets.
Francesco, Faggioli, Marta, Luigi
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Amplification of different nucleic acid targets in the same reaction (multiplex polymerase chain reaction) is challenging but an extremely useful tool especially for viroid diagnosis. In the amplification mixtures, several pairs of primers work together in the same conditions to detect different targets.
Francesco, Faggioli, Marta, Luigi
openaire +2 more sources
Multiplex PCR Design for Scalable Resequencing
2021While conventional PCR applications typically focus on a single PCR assay per reaction, multiplex PCR applications are a convenient and scalable solution becoming more routine. Multiplex methods can be applied to virtually any DNA template source (e.g., plant or human DNA, FFPE DNA isolated from clinical samples, bisulfite-converted DNA for DNA ...
Korbie, Darren, Trau, Matt
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Multiplex Real-Time PCR (MRT-PCR) for Diarrheagenic
2012Diarrheagenic Escherichia coli strains are important causes of diarrhea in children from the developing world and are now being recognized as emerging enteropathogens in the developed world. Current methods of detection are too expensive and labor-intensive for routine detection of these organisms to be practical.
Barletta, Francesca +2 more
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Optimization of Multiplex PCRs
1994The development of the polymerase chain reaction (PCR) has enabled rapid and efficient analysis of specific DNA sequences (Mullis and Faloona, 1987). Most PCR strategies are designed to amplify one or more target sequences with a single set of oligonucleotide primers.
Jeffrey S. Chamberlain +1 more
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