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Multiplex PCR for detection of Enterobacteriaceae in blood
Transfusion, 2001BACKGROUND: Rapid and sensitive methods are needed to detect the small numbers of bacteria that may sometimes contaminate units of blood during collection. A multiplex 5′‐nuclease TaqMan PCR assay (PE Applied Biosystems) was used to detect several bacterial species that may contaminate blood.STUDY DESIGN AND METHODS: Oligonucleotide primers were made ...
K, Sen, D M, Asher
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Real-Time Multiplex PCR Assays
Methods, 2001The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra.
C T, Wittwer +3 more
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Multiplex-Tandem PCR for Fungal Diagnostics
2012Multiplex, real-time PCR has become an invaluable tool for the rapid identification of pathogens in clinical specimens enabling earlier and more targeted management of antimicrobial therapy. In this chapter, we describe the methodology behind a novel multiplex-tandem PCR (MT-PCR) platform designed for the rapid identification of up to 18 fungal ...
Anna, Lau +2 more
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Multiplex PCR Amplification of Ancient DNA
2011Multiplex PCR allows the simultaneous amplification of up to dozens of target fragments in a single PCR. It is therefore a powerful tool to obtain many kilobases of continuous sequence from minute amounts of ancient DNA (aDNA), which usually must be amplified in multiple short and overlapping fragments.
Mathias, Stiller, Tara L, Fulton
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Optimizing multiplex and LA-PCR with betaine
Trends in Biochemical Sciences, 1997Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the internet. This month's column discusses the use of additives for optimizing the amount and quality of product obtained through multiplex and 'long and accurate' polymerase chain reaction (LA-PCR).
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