Disassembly of intact multiprotein complexes in the gas phase
Current Opinion in Structural Biology, 1999 The observation of multiprotein complexes by mass spectrometry formerly relied upon chemical cross-linking to maintain interactions. Recent technological developments have enabled the observation of intact macromolecular complexes without modification ...
Carol V Robinson
exaly +4 more sources
Visualization of Multiprotein Complexes by Flow Cytometry [PDF]
Current Protocols in Immunology, 2009 AbstractMultiprotein complexes and other protein‐protein interactions play important roles in virtually all cellular processes. Analysis of coimmunoprecipitation of protein complexes by flow cytometry (IP‐FCM, or “the fly‐p” method) provides a sensitive means to measure these interactions in the native/nondenatured state.
Adam G Schrum
exaly +5 more sources
UBE2O is a quality control factor for orphans of multiprotein complexes [PDF]
Science, 2017 Removing orphan proteins from the system The degradation of excess subunits of protein complexes is a major quality-control problem for the cell. How such “orphans” are recognized and tagged for degradation is poorly understood.
Kota Yanagitani +2 more
exaly +5 more sources
Multiprotein GLI Transcriptional Complexes as Therapeutic Targets in Cancer
Life, 2022 The Hedgehog signaling pathway functions in both embryonic development and adult tissue homeostasis. Importantly, its aberrant activation is also implicated in the progression of multiple types of cancer, including basal cell carcinoma and ...
Fan Yang +4 more
doaj +3 more sources
MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes. [PDF]
Methods Enzymol, 2017 Recombinant expression of large, multiprotein complexes is essential and often rate limiting for determining structural, biophysical, and biochemical properties of DNA repair, replication, transcription, and other key cellular processes.
Gradia SD +5 more
europepmc +2 more sources
Fine-Tuning Multiprotein Complexes Using Small Molecules [PDF]
ACS Chemical Biology, 2012 Multiprotein complexes such as the transcriptional machinery, signaling hubs, and protein folding machines are typically composed of at least one enzyme combined with multiple non-enzymes.
Mapp, Anna K +3 more
core +6 more sources
Gap junctional channels are parts of multiprotein complexes
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2012 Gap junctional channels are a class of membrane channels composed of transmembrane channel-forming integral membrane proteins termed connexins, innexins or pannexins that mediate direct cell-to-cell or cell-to extracellular medium communication in almost
Giepmans, Ben N. G. +6 more
core +3 more sources
Multicolour fluorescence-detection size-exclusion chromatography for structural genomics of membrane multiprotein complexes. [PDF]
PLoS ONE, 2013 Many interesting and important membrane proteins are hetero-oligomeric. However, besides naturally abundant examples, the structures of relatively few such complexes are known.
David Parcej +4 more
doaj +2 more sources
A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells
Cell Reports, 2015 Conventional affinity purification followed by mass spectrometry (AP-MS) analysis is a broadly applicable method used to decipher molecular interaction networks and infer protein function.
Mathieu Dalvai +7 more
doaj +3 more sources
U-insertion/deletion RNA editing multiprotein complexes and mitochondrial ribosomes in Leishmania tarentolae are located in antipodal nodes adjacent to the kinetoplast DNA. [PDF]
Mitochondrion, 2015 We studied the intramitochondrial localization of several multiprotein complexes involved in U-insertion/deletion RNA editing in trypanosome mitochondria.
Wong RG +5 more
europepmc +2 more sources