Results 41 to 50 of about 37,255 (173)

Multiprotein complex formation on the c‐myc promoter [PDF]

IUBMB Life, 1997
AbstractThe E2F element is a cis‐acting DNA sequence within the P2 promoter of the c‐myc proto‐oncogene. While it is required for optimal transcription, the multiprotein complexes formed on this site have not been well characterized. We show that in extracts of human glioblastoma cells and NIH3T3 fibroblasts, significant E2F transcription factor ...
T J, Logan, K H, Moberg, D J, Hall
openaire   +2 more sources

Multiprotein Expression Strategy for Structural Biology of Eukaryotic Complexes [PDF]

Structure, 2007
The concept of the cell as a collection of multisubunit protein machines is emerging as a cornerstone of modern biology, and molecular-level study of these machines in most cases will require recombinant production. Here, we present and validate a strategy to rapidly produce, permutate, and posttranslationally modify large, eukaryotic multiprotein ...
Fitzgerald, Daniel J, Schaffitzel, Christiane, Berger, Philipp, Wellinger, Ralf, Bieniossek, Christoph, Richmond, Timothy J, Berger, Imre   +6 more
openaire   +4 more sources

Interplay between circadian and other transcription factors—Implications for cycling transcriptome reprogramming

FEBS Letters, EarlyView.
This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley   +1 more source

Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes

Nature Communications
Most cellular processes are carried out by multiprotein assemblies. Although various molecular tools exist to visualize binary protein interactions in live cells, the visualization of multiprotein complexes remains a challenge.
Sara Bottone, Fanny Broch, Antoine Gedeon, Aurélien Brion, Lina El Hajji, Hela Benaissa, Arnaud Gautier   +6 more
doaj   +1 more source

Targeted tandem affinity purification of PSD‐95 recovers core postsynaptic complexes and schizophrenia susceptibility proteins

Molecular Systems Biology, 2009
The molecular complexity of mammalian proteomes demands new methods for mapping the organization of multiprotein complexes. Here, we combine mouse genetics and proteomics to characterize synapse protein complexes and interaction networks.
Esperanza Fernández, Mark O Collins, Rachel T Uren, Maksym V Kopanitsa, Noboru H Komiyama, Mike D R Croning, Lysimachos Zografos, J Douglas Armstrong, Jyoti S Choudhary, Seth G N Grant   +9 more
doaj   +1 more source

Metabologenomic Hallmark‐Based Discovery of Bacterial Thioamides as a New Lead against Drug‐Resistant Pancreatic Cancer

Advanced Science, EarlyView.
A targeted metabologenomic strategy integrating genomic and metabolomic hallmarks enabled the discovery of two new thioamides, thiogochangamides A and B, from bacteria. Their absolute configurations, previously unresolved within the thioviridamide family, were fully assigned through various chemical derivatizations and computational analysis ...
Young Eun Du, Eun Seo Bae, Thinh T. M. Bui, Seok Beom Lee, Dahan Kim, Jin‐Gyeong Park, Yongjoo Park, Soo Yeon Park, Sangwook Kang, Beomsu Lee, Daniel Shin, Yun Pyo Kang, In‐Gyun Lee, Dae‐Duk Kim, Seokhee Kim, Suckchang Hong, Kyuho Moon, Sang Kook Lee, Dong‐Chan Oh   +18 more
wiley   +1 more source

Protocol for the Analysis of Yeast and Human Mitochondrial Respiratory Chain Complexes and Supercomplexes by Blue Native Electrophoresis

STAR Protocols, 2020
Summary: By using negatively charged Coomassie brilliant blue G-250 dye to induce a charge shift on proteins, blue native polyacrylamide gel electrophoresis (BN-PAGE) allows resolution of enzymatically active multiprotein complexes extracted from ...
Alba Timón-Gómez, Rafael Pérez-Pérez, Eva Nyvltova, Cristina Ugalde, Flavia Fontanesi, Antoni Barrientos   +5 more
doaj   +1 more source

The inner workings of the hydrazine synthase multiprotein complex [PDF]

Nature, 2015
Anaerobic ammonium oxidation (anammox) has a major role in the Earth's nitrogen cycle and is used in energy-efficient wastewater treatment. This bacterial process combines nitrite and ammonium to form dinitrogen (N2) gas, and has been estimated to synthesize up to 50% of the dinitrogen gas emitted into our atmosphere from the oceans.
Andreas Dietl, Christina Ferousi, Wouter J. Maalcke, Andreas Menzel, Simon de Vries, Jan T. Keltjens, Mike S. M. Jetten, Boran Kartal, Thomas R. M. Barends   +8 more
openaire   +4 more sources

Rapid Proteome‐Wide Discovery of Protein–Protein Interactions With ppIRIS

Advanced Science, EarlyView.
ppIRIS is a lightweight deep learning framework for proteome‐wide protein–protein interaction prediction directly from sequence. By fusing evolutionary and structural embeddings with a regularized Siamese architecture, ppIRIS achieves state‐of‐the‐art accuracy across species, enables minute‐scale screening, and reveals biologically validated bacterial ...
Luiz Felipe Piochi, Di Tang, Johan Malmström, Yasaman Karami, Hamed Khakzad   +4 more
wiley   +1 more source

Revealing Protein–Protein Interactions Using a Graph Theory‐Augmented Deep Learning Approach

Advanced Intelligent Discovery, EarlyView.
This study presents a fast, cost‐efficient approach for classifying protein–protein interactions by integrating graph‐theory parametrization with deep learning (DL). Multiscale features extracted from graph‐encoded polarized‐light microscopy (PLM) images enable accurate prediction of binding strengths.
Bahar Dadfar, Gözde Kabay, Matthias Franzreb, Kody Whisnant, Nicholas A. Kotov, Joerg Lahann   +5 more
wiley   +1 more source