Results 151 to 160 of about 45,003 (206)

Optimizing In Vivo CAR-T Cell Engineering for Cancer Immunotherapy. [PDF]

Cancer Res
Wang R, Yu J, Caligiuri MA, Ma S.
europepmc   +1 more source

An improved lentivirus vector for safer transgene integration and protein transduction [PDF]

Schenkwein, Diana
core  

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T-DNA insertion mutagenesis.

2012
This chapter focuses on the significance of insertional mutagenesis in functional genomic studies and summarizes T-DNA mutagenesis schemes, including methodologies for creation of T-DNA mutant collections, T-DNA vectors, isolation of the tagged gene and comparisons of the frequency of mutant recovery using various strategies. Concrete examples are also
Sangwan, Rajbir S., Ochatt, Sergio, Nava-Saucedo, José-Edmundo, Sangwan-Norreel, Brigitte   +3 more
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Gene Therapy Insertional Mutagenesis Insights

Science, 2004
In a milestone study describing the first “cure” of a genetic disease by retroviral gene therapy, 9 out of 10 infants born with X-linked severe combined immunodeficiency (SCID-X1, also known as γc or IL2RG deficiency) were successfully treated with autologous bone marrow stem cells infected ...
Utpal P, Davé, Nancy A, Jenkins, Neal G, Copeland   +2 more
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Insertional mutagenesis of pathogenic fungi

Current Opinion in Microbiology, 1998
Screening insertional mutants for loss of virulence is an effective method for investigating the molecular genetic basis of bacterial pathogenesis, but has only recently been applied to fungal pathogens. For many pathogenic fungi transformation with heterologous plasmid DNA results in complex integration events. This problem can now be circumvented for
J S, Brown, D W, Holden
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Controlling P element insertional mutagenesis

Trends in Genetics, 1988
Abstract Two new methods for insertional mutagenesis in Drosophila are now available. They are based on the ability to control experimentally the number of stable P element insertions per mutagenized strain. One method produces about 10 insertions per genome and has the potential to replace hybrid dysgenesis as a means for cloning genes by ...
L, Cooley, C, Berg, A, Spradling
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Insertion mutagenesis of Escherichiacoli GroEL

Biochemical and Biophysical Research Communications, 2003
To gain insights into the in vivo folding and assembly of bacterial chaperonins, groEL was subjected to insertion mutagenesis using transposon ISlacZ/in. Four GroEL-LacZ fusions and the corresponding insertion mutants were obtained after residues 34, 90, 291, and 367. Apical domain insertion mutants GroEL291 and GroEL367 were degraded into monomeric 30-
Danielle, Amatore, François, Baneyx
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Insertional mutagenesis in transgenic mice

Transgenic Research, 1994
Increasing numbers of transgenic mouse lines have resulted in several dozens of mutants created by insertional mutagenesis. The advantages of different vector systems and the problems associated with the analysis of mutations and the cloning of the affected genes are discussed in this review.
T, Rijkers, A, Peetz, U, Rüther
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Insertional mutagenesis of Aspergillus fumigatus

Molecular and General Genetics MGG, 1998
We have investigated transformation with heterologous DNA as a method for insertional mutagenesis of Aspergillus fumigatus. Two methods, polyethylene glycol-mediated transformation of protoplasts and electroporation of germinating spores, were used to establish conditions leading to single-copy integration of transforming DNA at different genomic sites.
J S, Brown, A, Aufauvre-Brown, D W, Holden   +2 more
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Ty insertional mutagenesis

1992
Abstract The use of transposable elements as insertional mutagens is an extremely powerful technology for genetic analysis. This approach was brought to a fine art in prokaryotic genetics and is proving equally applicable in fruit flies, mice, and higher plants.
J N Strathern, M Mastrangelo, L A Rinckel,, D J Garfinkel   +3 more
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