Results 71 to 80 of about 28,635 (297)

Detection of Protein–Protein Interactions in Escherichia coli With Single Molecule Sensitivity

Advanced Science, EarlyView.
This article describes a regulatory circuit in Escherichia coli able to detect protein–protein interactions with exquisite sensitivity. The interaction between two hybrid proteins fused to Bordetella pertussis adenylate cyclase and its activator calmodulin triggers a potent cyclic AMP signaling cascade.
Marilyne Davi, Daniel Ladant
wiley   +1 more source

Redox‐Dependent Chaperoning of GBF1 Condensates Regulates Seed Germination in Arabidopsis

Advanced Science, EarlyView.
In dormant seeds (low ROS), GBF1 forms liquid condensates to repress the germination gene CathB3, and the chaperone GIP1 maintains condensate liquidity and repressive activity. Upon imbibition (high ROS), ROS oxidize GIP1 during germination, impairing its chaperone function.
Yunying Wang, Xiaofeng Fang
wiley   +1 more source

FSTVAL: a new web tool to validate bulk flanking sequence tags

Plant Methods, 2012
Background Information about a transgene locus is one of the major concerns in transgenic research because expression of the transgene or a gene interrupted by the integration event could be affected. Thus, the flanking sequences obtained from transgenic
Kim Joung, Kim Jiye, Lee Tae-Ho, Jun Kyong, Kim Tea, Kim Yul-Ho, Park Hyang-Mi, Jeon Jong-Seong, An Gynheung, Yoon Ung-Han, Nahm Baek, Kim Yeon-Ki   +11 more
doaj   +1 more source

Lentiviral vector-based insertional mutagenesis identifies genes associated with liver cancer

, 2013
Transposons and gamma-retroviruses have been efficiently used as insertional mutagens in different tissues to identify molecular culprits of cancer. However, these systems are characterized by recurring integrations that accumulate in tumor cells and ...
von Kalle Christof, Cesana Daniela, Pala Mauro, Kim Yoon Jun, Schmidt Manfred, DOGLIONI , CLAUDIO, Sergi Lucia Sergi, NALDINI , LUIGI, Bulfones Alessandro, Tonon Giovanni, Benedicenti Fabrizio, Gallina Pierangela, Montini Eugenio, Ranzani Marco, Sanvito Francesca, Merella Stefania, Bartholomae Cynthia C.   +16 more
core   +1 more source

Combining Fret and Super‐Resolution Microscopy Reveals Kinase Activation and Mitochondrial Activity at the Nanoscale

Advanced Science, EarlyView.
BioSenSRRF is an open‐source workflow that combines conventional FRET biosensors with SRRF reconstruction to generate sub‐diffraction FRET index maps on standard microscopes. The pipeline integrates automated image registration, SRRF reconstruction, quantitative FRET index calculation, and random line‐based hotspot analysis to uncover AURKA‐dependent ...
Nicolas Y. Jolivet, Pierre‐Jean Desmaison, Xavier Pinson, Arthur Masson, Olivier Delalande, Giulia Bertolin   +5 more
wiley   +1 more source

Light controls growth and development via a conserved pathway in the fungal kingdom. [PDF]

PLoS Biology, 2005
Light inhibits mating and haploid fruiting of the human fungal pathogen Cryptococcus neoformans, but the mechanisms involved were unknown. Two genes controlling light responses were discovered through candidate gene and insertional mutagenesis approaches.
Alexander Idnurm, Joseph Heitman
doaj   +1 more source

Endogenous Engineering Reprograms Extracellular Vesicles for Enhanced Therapeutic Function

Advanced Science, EarlyView.
This review explains how Extracellular vesicles‐producing cells can be endogenously engineered to load therapeutic proteins and nucleic acids. We summarize physiological and genetic strategies that harness native sorting pathways for selective cargo loading.
Jinghui Wang, Wenxuan Zhao, Xiaoming Hu, Yishu Wang, Peidong Yang, Diwei Zheng, Yugang Wang, Guanghui Ma, Min Shi, Wei Wei, Shuang Wang   +10 more
wiley   +1 more source

Conditional ablation of the RFX4 isoform 1 transcription factor: Allele dosage effects on brain phenotype. [PDF]

PLoS ONE, 2018
Regulatory factor X4 (RFX4) isoform 1 is a recently discovered isoform of the winged helix transcription factor RFX4, which can bind to X-box consensus sequences that are enriched in the promoters of cilia-related genes.
Ping Xu, James P Morrison, Julie F Foley, Deborah J Stumpo, Toni Ward, Darryl C Zeldin, Perry J Blackshear   +6 more
doaj   +1 more source

A transgenic dTph1 insertional mutagenesis system for forward genetics in mycorrhizal phosphate transport of Petunia

, 2008
The active endogenous dTph1 system of the Petunia hybrida mutator line W138 has been used in several forward-genetic mutant screens that were based on visible phenotypes such as flower morphology and color. In contrast, defective symbiotic phosphate (Pi)
Wegmüller, Sarah, Svistoonoff, Sergio, Amrhein, Nikolaus, Marcel Bucher, Nikolaus Amrhein, Sarah Wegmüller, Stuurman, Jeroen, Sergio Svistoonoff, Reinhardt, Didier, Jeroen Stuurman, Bucher, Marcel, Didier Reinhardt   +11 more
core   +1 more source

Fine‐Tuned Regulation of mRNA Translation and Transport by STAU2 Condensate Facilitates Neuronal Development and Plasticity

Advanced Science, EarlyView.
STAU2 undergoes phase separation to form dynamic condensates that package target mRNAs and deliver them to the distal ends of growing neuronal dendrites. STAU2 condensates stabilize embedded mRNAs and repress their translation. Synaptic activity bidirectionally remodels STAU2 condensates, coordinating local translation of STAU2‐associated mRNAs ...
Shijing Huang, Yuanyuan Chu, Ruiqian Zhao, Siyu Li, Yu Feng, Hongdan Zheng, Yunli Xie, Tong Wang, Wenyu Wen   +8 more
wiley   +1 more source