Results 61 to 70 of about 28,635 (297)
Nonmycorrhizal (Myc¯) Mutants of Hebeloma cylindrosporum Obtained Through Insertional Mutagenesis
Molecular Plant-Microbe Interactions, 2004 Polyethylene glycol-mediated transformation of protoplasts was used as a method for insertional mutagenesis to obtain mutants of the ectomycorrhizal fungus Hebeloma cylindrosporum impaired in symbiotic ability.
Jean-Philippe Combier +5 more
doaj +1 more source
A study of mechanisms of genotoxicity in mammalian cells by retrovirus vectors intended for gene therapy [PDF]
, 2013 This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.Retrovirus gene therapy vectors can deliver therapeutic genes to mammalian cells in a permanent manner by integrating their genome into host chromosomes and
Reja, Safia
core
Insertional mutagenesis in Populus: Relevance and feasibility
, 2005 The recent sequencing of the first tree genome, that of the black cottonwood (Populus trichocarpa), opens a new chapter in tree functional genomics.
Fladung, Matthias +3 more
core +1 more source
Mutant NPM1 in Acute Myeloid Leukemia Initiation and Maintenance
Aging and Cancer, EarlyView.NPM1 mutations drive acute myeloid leukemia by acting as neomorphic transcriptional regulators that cooperate with Menin–MLL and XPO1 to sustain HOX/MEIS1 expression and block differentiation. Targeting these mutant‐specific transcriptional dependencies provides a rational therapeutic strategy for NPM1‐mutated AML.
Yanan Jiang +3 more
wiley +1 more source
Measurement of insertional mutagenesis by retroviral and lentiviral vectors. [PDF]
, 2008 Retroviral vectors have been successfully used in the clinic for the correction of inherited immunodeficiencies. However, in 2 recent gene therapy trials for X-linked Severe Combined Immunodeficiency, 5 patients developed leukaemia.
Bokhoven, M.C. +1 more
core
, 2013 Occurrence of “positives” during insertional mutagenesis of ssrA using the temperature sensitive plasmid pGM160.
Chunzhong Yang (374567) +1 more
core +1 more source
Cracking the Code: Genotype–Phenotype Correlation Models in Sarcoglycanopathies
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Objective Sarcoglycanopathies are among the most severe limb‐girdle muscular dystrophies (LGMD), though milder presentations have been described. These diseases are primarily caused by missense variants, but the limited predictability of their effect on protein maturation, complex formation, and transport has hindered reliable genotype ...
Leonela Luce +72 more
wiley +1 more source
Modeling integration site data for safety assessment with MELISSA
Nature CommunicationsGene and cell therapies pose safety concerns due to potential insertional mutagenesis by viral vectors. We introduce MELISSA, a regression-based statistical framework for analyzing Integration Site (IS) data to assess insertional mutagenesis risk, by ...
Tsai-Yu Lin +9 more
doaj +1 more source
Targeted insertional mutagenesis libraries for deep domain insertion profiling [PDF]
Nucleic Acids Research, 2019 AbstractDomain recombination is a key principle in protein evolution and protein engineering, but inserting a donor domain into every position of a target protein is not easily experimentally accessible. Most contemporary domain insertion profiling approaches rely on DNA transposons, which are constrained by sequence bias.
Willow Coyote-Maestas +4 more
openaire +4 more sources
Enhanced gene trapping in mouse embryonic stem cells
, 2008 Gene trapping is used to introduce insertional mutations into genes of mouse embryonic stem cells (ESCs). It is performed with gene trap vectors that simultaneously mutate and report the expression of the endogenous gene at the site of insertion and ...
Hansen, J. +17 more
core +1 more source