Results 81 to 90 of about 28,635 (297)

A Graph Based Framework to Model Virus Integration Sites

Computational and Structural Biotechnology Journal, 2016
With next generation sequencing thousands of virus and viral vector integration genome targets are now under investigation to uncover specific integration preferences and to define clusters of integration, termed common integration sites (CIS), that may ...
Raffaele Fronza, Alessandro Vasciaveo, Alfredo Benso, Manfred Schmidt   +3 more
doaj   +1 more source

SDS‐CRISPR for Single‐Nucleotide Variant Detection

Advanced Science, EarlyView.
Structure‐disruption‐sensitive CRISPR (SDS‐CRISPR) converts structural instability into single‐nucleotide precision, thereby overcoming mismatch tolerance in canonical Cas12a and enabling versatile diagnostics across DNA, RNA, and microRNA targets. When applied to rapid IDH1 mutation detection for glioma genotyping and integrated with lateral‐flow ...
Xin Guan, Chong Guo, Jiongyu Zhang, Rui Yang, Yerramsetti Ramachandra, Chengyu Hou, Minjie Pei, Shuo Zhang, Kurt T. Schalper, Xingye Liu, Qian Wu, Ketan R. Bulsara, Changchun Liu   +12 more
wiley   +1 more source

Early stages of leaf development in has mutant of Arabidopsis thaliana

Biologia Plantarum, 2011
The elucidation of molecular mechanisms underlying the leaf development can be facilitated by the detailed anatomical study of leaf development mutants.
D. Janošević, B. Uzelac, D. Stojičić, Lj. Tubić, S. Budimir   +4 more
doaj   +1 more source

Transposon insertional mutagenesis of diverse yeast strains suggests coordinated gene essentiality polymorphisms

Nature Communications, 2022
Epistasis can lead to different phenotypic consequences from the same mutation. Here the authors carry out a genome-wide analysis of conditionally essential genes in yeast, finding that gene essentiality changes tend to occur concordantly among ...
Piaopiao Chen, Agnès H. Michel, Jianzhi Zhang   +2 more
doaj   +1 more source

Insertional mutagenesis of the mouse germline with Sleeping Beauty transposition [PDF]

, 2008
Efficient linking of primary DNA sequence information to gene functions in vertebrate models requires that genetic modifications and their effects are analyzed in an efficacious, controlled, and scalable manner.
Ivics, Z., Zsuzsanna Izsvák, Izsvak, Z., Junji Takeda, Takeda, J., Zoltán Ivics   +5 more
core   +1 more source

Covalent Reprogramming of Kinase Binders to Modulate Protein Abundance

Advanced Science, EarlyView.
Electrophilic remodeling of a broad‐spectrum kinase binder reveals how subtle chemical changes reprogram protein fate. An acrylamide analog of a multi‐kinase binder selectively stabilizes Aurora kinase A (AURKA) by suppressing its ubiquitination, while a short‐linker variant converts this stabilizer into a degrader.
Chen Mozes, Xiaokang Jin, Miguel A. Campos, Chen Zhou, Xiaoyu Zhang   +4 more
wiley   +1 more source

Morphophysiological alterations caused by insertional mutagenesis of contactin 5 (Cntn5) gene in transgenic mice

Вавиловский журнал генетики и селекции, 2017
Transgenesis has become a routine for modern biological studies. The most popular method for producing transgenic animals–pronuclear microinjection–frequently leads to host gene disruption due to a random transgene integration.
A. V. Smirnov, N. A. Feofanova, G. V. Kontsevaya, M. V. Anisimova, I. I. Kovrigin, I. A. Serova, M. P. Moshkin, L. A. Gerlinskaya, N. R. Battulin   +8 more
doaj   +1 more source

Secreted Nonstructural Protein 3 is a Pathogenic Determinant of Orbivirus

Advanced Science, EarlyView.
This study uncovers a conserved PIP2‐dependent secretory pathway of orbivirus NS3 that induces vascular leakage. Pharmacological disruption of PIP2‐NS3 interaction significantly reduces viral pathogenicity and provides protective efficacy in murine models, establishing PIP2‐mediated NS3 secretion as both a key virulence determinant and a promising ...
Junyong Guan, Dong Zhou, Ran Shao, Xing Liu, Jin Peng, Yingran Huang, Shuhui Qi, Cankun Xi, Menghang Wang, Bin Sun, Yinglin Qi, Xin Yin   +11 more
wiley   +1 more source

THE ROLE OF mutR GENE IN METABOLISM AND VIRULENCE OF emm12 GENOTYPE STREPTOCOCCUS PYOGENES STRAINS

Инфекция и иммунитет, 2015
In the present study the functional role of the mutR regulatory protein gene of Streptococcus pyogenes (emm12) was studied. The mutR gene was inactivated in the strains no. 97 and no. 152 by insertional mutagenesis.
A. A. Zutkis, B. L. Milman, A. V. Dmitriev   +2 more
doaj   +1 more source

Random mutagenesis in Corynebacterium glutamicum ATCC 13032 using an IS6100-based transposon vector identified the last unknown gene in the histidine biosynthesis pathway

, 2006
Mormann S, Lömker A, Rückert C, et al. Random mutagenesis in Corynebacterium glutamicum ATCC 13032 using an IS6100-based transposon vector identified the last unknown gene in the histidine biosynthesis pathway. BMC Genomics.
Mormann, Sascha, Rückert, Christian ; https://orcid.org/, Tauch, Andreas, Lömker, Alexander, Gaigalat, Lars, Pühler, Alfred ; https://orcid.org/, Kalinowski, Jörn   +6 more
core   +1 more source