Results 61 to 70 of about 291 (106)

Organ distinctive mutagenicity in Muta^TMMouse after short-term exposure to PhIP [PDF]

Mutagenesis, 1996
We have investigated PhIP-induced mutagenicity in various tissues (kidney, liver, large and small intestine) using a transgenic mouse model (MutaMouse). In addition to tissue specific mutagenesis, we measured the binding of [14C]PhIP to MutaMouse mice blood proteins (haemoglobin and albumin), to obtain a quantitative estimate of carcinogen exposure and
A M, Lynch, N J, Gooderham, A R, Boobis
openaire   +2 more sources

Induction of lacZ mutations in Muta™Mouse primary hepatocytes [PDF]

Environmental and Molecular Mutagenesis, 2009
AbstractWe have developed an in vitro mutation assay using primary hepatocytes from the transgenic Muta™Mouse. Primary hepatocytes were isolated using a two‐step perfusion method with purification by Percoll, cultured, and treated with benzo[a]pyrene (BaP), 2‐amino‐1‐methyl‐6‐phenyl‐ imidazo[4,5‐b]pyridine (PhIP), 3‐nitrobenzoanthrone (3‐NBA), and ...
Chen, Guosheng, Gingerich, John, Soper, Lynda, Douglas, George R, White, Paul A   +4 more
openaire   +2 more sources

Toxicity of Polymeric-Based Non-Viral Vector Systems for Pulmonary siRNA Application [PDF]

, 2011
no ...
Andrea Beyerle, Thomas Kissel, Tobias Stoeger   +2 more
core   +2 more sources

Additional file 1 of In vivo mutagenicity assessment of orally treated tert-butyl hydroperoxide in the liver and glandular stomach of MutaMouse

, 2023
Additional file 1: Supplementary Table 1. Clinical observations in the gene mutation assay of tert-butyl hydroperoxide. Supplementary Table 2. Historical data of negative controls (Transgenic rodent gene mutation assay (lacZ assay)).
Murata, Yasumasa, Suzuki, Kenichiro, Shigeta, Yoshiyuki, Iso, Takako, Hirose, Nozomu, Umano, Takaaki, Horibata, Katsuyoshi, Sugiyama, Kei-ichi, Hirose, Akihiko, Masumura, Kenichi, Matsumoto, Mariko   +10 more
openaire   +1 more source

The application of the restriction site mutation assay to compare 1-ethyl-1-nitrosourea-induced mutations between the endogenous p53 gene and the transgenic LacZ gene in MutaMouse testes [PDF]

Mutagenesis, 2001
Transgenic mouse modelling has provided a new approach to study the various steps involved in spontaneous and induced mutagenesis in rodent somatic and germline tissues in vivo. However, the important question arises as to whether mutations occur at the same rate in transgenes as in endogenous genes.
H L, Song, G J, Jenkins, J, Ashby, H, Tinwell, J M, Parry   +4 more
openaire   +5 more sources

The role of cytochrome P450 enzymes in carcinogen activation and detoxication:an in vivo-in vitro paradox [PDF]

, 2018
Many chemical carcinogens require metabolic activation via xenobiotic-metabolising enzymes in order to exert their genotoxic effects. Evidence from numerous in-vitro studies, utilising reconstituted systems, microsomal fractions and cultured cells ...
Arlt, Volker M, Phillips, David H, Reed, Lindsay   +2 more
core   +2 more sources

Transcriptional profiling of the mouse hippocampus supports an NMDAR-mediated neurotoxic mode of action for benzo[a]pyrene [PDF]

, 2016
Benzo[a]pyrene (BaP) is a genotoxic carcinogen and a neurotoxicant. The neurotoxicity of BaP is proposed to arise from either genotoxicity leading to neuronal cell death, or perturbed expression of N-methyl-d-aspartate receptor (NMDAR) subunits.
Arlt, Volker M, Bowers, Wayne J, Chepelev, Nikolai L, Gagné, Rémi, Kuo, Byron, Long, Alexandra S, Phillips, David H, White, Paul A, Williams, Andrew, Yauk, Carole L   +9 more
core   +2 more sources

Integration of In Vivo Genotoxicity and Short-term Carcinogenicity Assays Using F344 gpt Delta Transgenic Rats: In Vivo Mutagenicity of 2,4-Diaminotoluene and 2,6-Diaminotoluene Structural Isomers [PDF]

An important trend in current toxicology is the replacement, reduction, and refinement of the use of experimental animals (the 3R principle). We propose a model in which in vivo genotoxicity and short-term carcinogenicity assays are integrated with F344 ...
Akiyoshi Nishikawa, Hiroyuki Hayashi, Hisakazu Sanada, Kenichi Masumura, Makiko Takamune, Masami Yamada, Naomi Toyoda-Hokaiwado, National Toxicology Program, National Toxicology Program, Takashi Umemura, Takehiko Nohmi, Tomoki Inoue, Yasushi Kurata, Yuji Kawamura   +13 more
core   +2 more sources

Human Primordial Germ Cell Formation Is Diminished by Exposure to Environmental Toxicants Acting through the AHR Signaling Pathway [PDF]

, 2010
Historically, effects of environmental toxicants on human development have been deduced via epidemiological studies because direct experimental analysis has not been possible.
Cedars, Marcelle I., Flores, Martha, Kee, Kehkooi, Reijo Pera, Renee A.   +3 more
core   +3 more sources

Carbon black nanoparticle instillation induces sustained inflammation and genotoxicity in mouse lung and liver [PDF]

, 2012
Background Widespread occupational exposure to carbon black nanoparticles (CBNPs) raises concerns over their safety. CBNPs are genotoxic in vitro but less is known about their genotoxicity in various organs in vivo.
Bourdon, Julie A, Jacobsen, Nicklas R, Jensen, Keld A, Lamson, Jacob S, Loft, Steffen, Madsen, Anne M, Møller, Peter, Saber, Anne T, Vogel, Ulla B, Wallin, Håkan, Yauk, Carole L   +10 more
core   +3 more sources