Results 51 to 60 of about 260 (125)

Sequencing Chemically Induced Mutations in the Mutamouse Lacz Reporter Gene Identifies Human Cancer Mutational Signatures [PDF]

, 2019
ABSTRACT Transgenic rodent (TGR) models use bacterial reporter genes to quantify in vivo mutagenesis. Pairing TGR assays with next-generation sequencing (NGS) enables comprehensive mutation spectrum analysis to inform mutational mechanisms.
Beal, Marc A., Meier, Matt J., LeBlanc, Danielle, Maurice, Clotilde, O’Brien, Jason, Yauk, Carole L., Marchetti, Francesco   +6 more
openaire   +1 more source

Subchronic oral exposure to benzo(a)pyrene leads to distinct transcriptomic changes in the lungs that are related to carcinogenesis [PDF]

, 2012
We have previously shown that acute oral exposure to the environmental carcinogen benzo(a)pyrene (BaP) elicits comparable levels of DNA adducts, but distinct transcriptomic changes, in mouse lungs and livers, the two main BaP bioactivating organs.
Labib, Sarah, Williams, Andrew, Arlt, Volker M.; id_orcid, Halappanavar, Sabina, Yauk, Carole, Phillips, David H., White, Paul A.   +6 more
core   +1 more source

Re‐evaluation of sucralose (E 955) as a food additive and evaluation of a new application on extension of use of sucralose (E 955) in fine bakery wares

EFSA Journal, Volume 24, Issue 2, February 2026.
Abstract The present opinion deals with the re‐evaluation of sucralose (E 955) as food additive and with the safety of a proposed extension of use in food category (FC) 7.2 ‘Fine bakery wares’. Based on the available data, no safety concerns arose for genotoxicity of sucralose (E 955) and its impurities and degradation products.
EFSA Panel on Food Additives and Flavourings (FAF), Laurence Castle, Monica Andreassen, Gabriele Aquilina, Maria Lourdes Bastos, Polly Boon, Biagio Fallico, Rex FitzGerald, Maria Jose Frutos Fernandez, Bettina Grasl‐Kraupp, Ursula Gundert‐Remy, Rainer Gürtler, Eric Houdeau, Marcin Kurek, Henriqueta Louro, Patricia Morales, Sabina Passamonti, Monika Batke, Antonios Douros, James Chipman, Riccardo Crebelli, Peter Fürst, Thorhallur Halldorsson, Manuela Mirat, Alicja Mortensen, Matthew Wright, Oliver Lindtner, Stefania Barmaz, Consuelo Civitella, Zsuzsanna Horvath, Sara Levorato, Elena Mazzoli, Josef Daniel Rasinger, Ana Maria Rincon, Camilla Smeraldi, Alexandra Tard, Federica Lodi   +36 more
wiley   +1 more source

Mutagenitätsprüfung mit dem in vitro-TGRA: von Frequenzen zu Signaturen [PDF]

The in vitro Transgenic Rodent Assay (TGRA) using metabolically competent primary MutaMouse hepatocytes represents a novel method for detecting gene mutations.
Honarvar, Naveed, Landsiedel, Robert, Göpfert, Alina, Funk-Weyer, Dorothee   +3 more
core   +1 more source

Interpretation of in vitro concentration‐response data for risk assessment and regulatory decision‐making: Report from the 2022 IWGT quantitative analysis expert working group meeting

Environmental and Molecular Mutagenesis, Volume 66, Issue S2, Page 101-120, December 2025.
Abstract Quantitative risk assessments of chemicals are routinely performed using in vivo data from rodents; however, there is growing recognition that non‐animal approaches can be human‐relevant alternatives. There is an urgent need to build confidence in non‐animal alternatives given the international support to reduce the use of animals in toxicity ...
Marc A. Beal, Guangchao Chen, Kerry L. Dearfield, Min Gi, Bhaskar Gollapudi, Robert H. Heflich, Katsuyoshi Horibata, Alexandra S. Long, David P. Lovell, Barbara L. Parsons, Stefan Pfuhler, John Wills, Andreas Zeller, George Johnson, Paul A. White   +14 more
wiley   +1 more source

Genetic analysis of PHIP intestinal mutations in Muta^TMMouse [PDF]

Mutagenesis, 1998
The mutagenicity of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) was investigated in male MutaMouse mice administered 20 mg/kg per o.s. for 4 days and killed 7 days later. Genomic DNA was extracted from liver, kidney and small and large intestine and the mutation frequency (MF) at the lacZ locus was determined using a positive selection assay.
A M, Lynch, N J, Gooderham, D S, Davies, A R, Boobis   +3 more
openaire   +2 more sources

Severity of effect considerations regarding the use of mutation as a toxicological endpoint for risk assessment: A report from the 8th International Workshop on Genotoxicity Testing (IWGT)

Environmental and Molecular Mutagenesis, Volume 66, Issue S2, Page 121-143, December 2025.
Abstract Exposure levels without appreciable human health risk may be determined by dividing a point of departure on a dose–response curve (e.g., benchmark dose) by a composite adjustment factor (AF). An “effect severity” AF (ESAF) is employed in some regulatory contexts.
Barbara L. Parsons, Marc A. Beal, Kerry L. Dearfield, George R. Douglas, Min Gi, B. Bhaskar Gollapudi, Robert H. Heflich, Katsuyoshi Horibata, Michelle Kenyon, Alexandra S. Long, David P. Lovell, Anthony M. Lynch, Meagan B. Myers, Stefan Pfuhler, Alisa Vespa, Andreas Zeller, George E. Johnson, Paul A. White   +17 more
wiley   +1 more source

The application of the restriction site mutation assay to compare 1-ethyl-1-nitrosourea-induced mutations between the endogenous p53 gene and the transgenic LacZ gene in MutaMouse testes [PDF]

Mutagenesis, 2001
Transgenic mouse modelling has provided a new approach to study the various steps involved in spontaneous and induced mutagenesis in rodent somatic and germline tissues in vivo. However, the important question arises as to whether mutations occur at the same rate in transgenes as in endogenous genes.
H L, Song, G J, Jenkins, J, Ashby, H, Tinwell, J M, Parry   +4 more
openaire   +5 more sources

Long term exposure to benzo[b]fluoranthene does not induce mutations in Mutamouse male germ cells

Reproductive Toxicology
Germ cell mutations can be inherited and lead to genetic disorders in the offspring. Thus, it is critical to identify environmental exposures that impact the germline. Polycyclic aromatic hydrocarbons (PAHs) are widespread combustion by-products found in food, tobacco smoke, and urban air.
Madison T. Stewart, Gu Zhou, Danielle P.M. LeBlanc, Annette E. Dodge, Matthew J. Meier, Andrew Williams, Alexandra S. Long, Paul A. White, Carole L. Yauk, Francesco Marchetti   +9 more
openaire   +2 more sources

Re‐evaluation of silicon dioxide (E 551) as a food additive in foods for infants below 16 weeks of age and follow‐up of its re‐evaluation as a food additive for uses in foods for all population groups

EFSA Journal, Volume 22, Issue 10, October 2024.
Abstract The present opinion is the follow‐up of the conclusions and recommendations of the Scientific Opinion on the re‐evaluation of silicon dioxide (E 551) as a food additive relevant to the safety assessment for all age groups. In addition, the risk assessment of silicon dioxide (E 551) for its use in food for infants below 16 weeks of age is ...
EFSA Panel on Food Additives and Flavourings (FAF), Maged Younes, Gabriele Aquilina, Laurence Castle, Gisela Degen, Karl‐Heinz Engel, Paul Fowler, Maria Jose Frutos Fernandez, Peter Fürst, Rainer Gürtler, Trine Husøy, Melania Manco, Wim Mennes, Peter Moldeus, Sabina Passamonti, Romina Shah, Ine Waalkens‐Berendsen, Matthew Wright, Cristina Andreoli, Maria Bastos, Diane Benford, Margherita Bignami, Claudia Bolognesi, Karlien Cheyns, Emanuela Corsini, Riccardo Crebelli, Birgit Dusemund, Rex Fitzgerald, Eric Gaffet, Katrin Loeschner, Francesca Marcon, Jan Mast, Manuela Mirat, Alicja Mortensen, Agnes Oomen, Josef Schlatter, Dominique Turck, Beate Ulbrich, Anna Undas, Christiane Vleminckx, Detlef Woelfle, Ruud Woutersen, Stefania Barmaz, Borana Dino, Gabriele Gagliardi, Sara Levorato, Elena Mazzoli, Alexis Nathanail, Ana Maria Rincon, Laura Ruggeri, Camilla Smeraldi, Alexandra Tard, Sam Vermeiren, Ursula Gundert‐Remy   +53 more
wiley   +1 more source