Results 61 to 70 of about 1,574,250 (305)

Structural implications of BK polyomavirus sequence variations in the major viral capsid protein Vp1 and large T-antigen: a computational study

mSphere
BK polyomavirus (BKPyV) is a double-stranded DNA virus causing nephropathy, hemorrhagic cystitis, and urothelial cancer in transplant patients. The BKPyV-encoded capsid protein Vp1 and large T-antigen (LTag) are key targets of neutralizing antibodies and
Janani Durairaj, Océane M. Follonier, Karoline Leuzinger, Leila T. Alexander, Maud Wilhelm, Joana Pereira, Caroline A. Hillenbrand, Fabian H. Weissbach, Torsten Schwede, Hans H. Hirsch   +9 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

‘Miyagawa’ New Bud Mutant Type: Enhances Resistance to Low-Temperature Stress

Agronomy
Global climate change is leading to more frequent extreme cold events, underscoring the need to study citrus cold tolerance to support breeding and enable potential northward expansion of citrus cultivation.
Shuangyou Wang, Yingzi Zhang, Ben Zhang, Weiqi Luo, Xiang Liu, Suming Dai, Dazhi Li, Na Li   +7 more
doaj   +1 more source

The Mutagenesis of Lactobacillus Thermophilus for Enhanced L-(+)-Lactic Acid Accumulation Induced by Heavy Ion Irradiation

Brazilian Archives of Biology and Technology, 2018
Screening promising L. thermophiles with high productivity, high efficiency and strong adaptability are very important in lactic acid industry. For this purpose, 80MeV/u carbon ions were applied to irradiate L. thermophiles.
Wei Hu, Jihong Chen, Qinghua Wu, Wenjian Li, Jing Liu, Dong Lu, Shuyang Wang   +6 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Comprehensive mapping and modelling of the rice regulome landscape unveils the regulatory architecture underlying complex traits

Nature Communications
Unraveling the regulatory mechanisms that govern complex traits is pivotal for advancing crop improvement. Here we present a comprehensive regulome atlas for rice (Oryza sativa), charting the chromatin accessibility across 23 distinct tissues from three ...
Tao Zhu, Chunjiao Xia, Ranran Yu, Xinkai Zhou, Xingbing Xu, Lin Wang, Zhanxiang Zong, Junjiao Yang, Yinmeng Liu, Luchang Ming, Yuxin You, Dijun Chen, Weibo Xie   +12 more
doaj   +1 more source

Intraperitoneal infection of A/J and CD1 mice with coxsackievirus B2 and its mutants

Acta Virologica
Coxsackieviruses (CVs) belong to the genus Enterovirus and family Picornaviridae. Mutants can arise within the replication cycle of RNA viruses. The prototype CVB2 Ohio-1 (CVB2/O) strain adapted to rhabdomyosarcoma (RD) cells induced cytolytic infection ...
Maria Borsanyiova, Katarina Berakova, Brigita Benkoova, Michaela Pellerova, A. Michael Lindberg, Shubhada Bopegamage   +5 more
doaj   +1 more source

Identification of Two New Mechanisms That Regulate Fruit Growth by Cell Expansion in Tomato

Frontiers in Plant Science, 2017
Key mechanisms controlling fruit weight and shape at the levels of meristem, ovary or very young fruit have already been identified using natural tomato diversity. We reasoned that new developmental modules prominent at later stages of fruit growth could
Constance Musseau, Daniel Just, Joana Jorly, Frédéric Gévaudant, Annick Moing, Christian Chevalier, Martine Lemaire-Chamley, Christophe Rothan, Lucie Fernandez   +8 more
doaj   +1 more source

Defining the requirements for the pathogenic interaction between mutant calreticulin and MPL in MPN [PDF]

, 2018
Mutations in calreticulin (CALR) are phenotypic drivers in the pathogenesis of myeloproliferative neoplasms. Mechanistic studies have demonstrated that mutant CALR binds to the thrombopoietin receptor MPL, and that the positive electrostatic charge of ...
Gabriel Birrane, Nouran S. Abdelfattah, Edwin Chen, Mullally, A., Rivera, JF, Natalie Florescu, Danielle Beeson, Chen, E, Chen, E., Ko, A, Elf, S, Abdelfattah, N.S., Jeanne F. Rivera, Florescu, N, Mullally, A, Elf, S., Florescu, N., Ann Mullally, Birrane, G., Ko, A., Shannon Elf, April J. Baral, Birrane, G, Baral, A.J., Beeson, D., Baral, AJ, Amy Ko, Abdelfattah, NS, Beeson, D, Rivera, J.F.   +29 more
core   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source