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Ginsenoside Rg1 from Panax ginseng enhances myoblast differentiation and myotube growth
Journal of Ginseng Research, 2017 Background: Ginsenoside Rg1 belongs to protopanaxatriol-type ginsenosides and has diverse pharmacological activities. In this report, we investigated whether Rg1 could upregulate muscular stem cell differentiation and muscle growth.
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Biolistic Transfection of Cultured Myotubes
Science Signaling, 2003Transfection of cells in culture with cDNA constructs is a powerful tool in cell biology, but postmitotic cells, including myotubes, can be hard to transfect with classic methods. Biolistics provides an alternative. We have used this biolistic technique to introduce cDNAs into cultured rat, chick, and C2C12 myotubes. This protocol results in efficient (
Christian, Antolik +2 more
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Electrical Impedance as a Novel Biomarker of Myotube Atrophy and Hypertrophy
SLAS Discovery, 2011 Measuring myotube thickness is the most physiological and unbiased approach for screening therapeutic compounds that prevent skeletal muscle atrophy or induce hypertrophy.
Yama A Abassi, David J Glass
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Serum regulation of acetylcholinesterase in cultured myotubes
Experimental Neurology, 1986A large (20S) collagen-tailed form of acetylcholinesterase associated with the neuromuscular junction appears in cultures of chick embryo muscle cells when horse serum is withdrawn from the medium. In this report, 10-day-old cultures were incubated 2 days in serum-free medium or in medium containing either horse, bovine, fetal calf, chicken, heat ...
G T, Patterson, B W, Wilson
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Journal of Child Neurology, 1992
Investigation of the congenital myopathies has been limited by a lack of knowledge regarding basic mechanisms involved in normal myogenesis of human muscle and the relative rarity of these diseases. A newly recognized family of regulatory genes has been shown to be necessary for myogenesis to proceed to formation of normal mature muscle.
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Investigation of the congenital myopathies has been limited by a lack of knowledge regarding basic mechanisms involved in normal myogenesis of human muscle and the relative rarity of these diseases. A newly recognized family of regulatory genes has been shown to be necessary for myogenesis to proceed to formation of normal mature muscle.
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Formation of Myotubes in Aneural Rat Muscles
Developmental Biology, 1993Skeletal muscles formed in the absence of innervation are deficient in number of fibers, atrophic, and prone to degeneration. We and others have suggested that primary myotubes form autonomously, and the major effect of embryonic denervation is to halt the formation of secondary myotubes by removing the mitotic stimulus for generation of their ...
S J, Wilson, A J, Harris
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Caffeine contracture in the cultured chick myotube
Journal of Cellular Physiology, 1986AbstractA possible function of Ca store site in cultured chick myotubes was examined by recording contraction of the myotube with special reference to the effect of caffeine. Caffeine at low concentrations (below 1 mM), applied focally on the myotube through a micropipette with a pressure pulse, elicited focal contraction without membrane potential ...
K, Saito, E, Ozawa
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Myotube Assembly on Nanofibrous and Micropatterned Polymers
Nano Letters, 2006Skeletal muscle consists of parallel bundles of myotubes formed by the fusion of myoblasts. We fabricated nanofibrous and micropatterned polymers as cell culture substrates to guide the morphogenesis of muscular tissue. The nanoscale and microscale topographic features regulate cell and cytoskeleton alignment, myotube assembly, myotube striation, and ...
Ngan F, Huang +7 more
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Effect of metformin on myotube BCAA catabolism
Journal of Cellular Biochemistry, 2019AbstractMetformin has antihyperglycemic properties and is a commonly prescribed drug for type II diabetes mellitus. Metformin functions in part by activating 5′‐AMP‐activated protein kinase, reducing hepatic gluconeogenesis and blood glucose. Metformin also upregulates peroxisome proliferator‐activated receptor‐gamma coactivator‐1α (PGC‐1α).
Madison E. Rivera +2 more
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Neutrophils injure cultured skeletal myotubes
American Journal of Physiology-Cell Physiology, 2001The purpose of the study was to test the hypothesis that neutrophils can injure cultured skeletal myotubes. Human myotubes were grown and then cultured with human blood neutrophils. Myotube injury was quantitatively and qualitatively determined using a cytotoxicity (51Cr) assay and electron microscopy, respectively.
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