Broccoli Myrosinase cDNA Expression in Escherichia coli and Saccharomyces cerevisiae [PDF]
Biomolecules, 2022 Myrosinases (EC 3.2.1.147) are enzymes known for the generation of hydrolysis products that have a potential beneficial effect on human health. Their reaction mechanisms are widely studied, in order to improve and optimize secondary metabolite production
Carolina Curiqueo +2 more
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Herbivore induction of the glucosinolate-myrosinase defense system: major trends, biochemical bases and ecological significance [PDF]
, 2009 Like many other plant defense compounds, glucosinolates are present constitutively in plant tissues, but are also induced to higher levels by herbivore attack.
Gershenzon, J., Textor, S.
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Hijacking the Mustard-Oil Bomb: How a Glucosinolate-Sequestering Flea Beetle Copes With Plant Myrosinases [PDF]
Frontiers in Plant Science, 2021 Myrosinase enzymes play a key role in the chemical defense of plants of the order Brassicales. Upon herbivory, myrosinases hydrolyze the β-S-linked glucose moiety of glucosinolates, the characteristic secondary metabolites of brassicaceous plants, which ...
Theresa Sporer +6 more
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Journal of the agricultural chemical society of Japan, 1959 (1)動物(ほらがい肝臓)ミロスルファターゼはシニグリン, tetramethylsinigrin, tetraacetylsinigrinの何れにも作用してその硫酸エステル結合を切る事,之に対し植物(白からし)ミロシナーゼは,シニグリンには作用してその硫酸エステル結合及びチオグルコシッド結合を切るが, tetramethylsinigrin, tetraacetylsinigrinには全く作用しない事を知った. (2)阻害剤に対する性質も,植物ミロシナーゼのミロスルファターゼ作用と,動物ミロスルファターゼとでは,可成り異る事を明かにした.
Zenji NAGASHIMA, Masaaki Uchiyama
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A Simple Method for On-Gel Detection of Myrosinase Activity [PDF]
Molecules, 2018 Myrosinase is an enzyme present in many functional foods and spices, particularly in Cruciferous vegetables. It hydrolyses glucosinolates which thereafter rearrange into bioactive volatile constituents (isothiocyanates, nitriles).
Sándor Gonda +6 more
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Studies on the Myrosinase in Mustard Seed [PDF]
Agricultural and Biological Chemistry, 1967 In order to clarify whether the myrosinase containes only thioglucosidase, or thioglucosidase and sulfatase, chromatographic behaviors of the myrosinase on cellulose ion exchanger were studied. Myrosinase showed two peaks by the chromatography on TEAE-cellulose at pH 8.5, but the fractionation of the two enzymes was not accomplished.
Isao Tsuruo, Mizuho YOSHIDA, Tadao Hata
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Agricultural and Biological Chemistry, 1969 In order to obtain fungous myrosinase, Aspergillus sydowi IFO 4284 was cultured on a medium containing mustard seed extract for 2 weeks. Myrosinase in the broth was purified about 150 fold by precipitation with ammonium sulfate and chromatography on DEAE-cellulose and DEAE-Sephadex.
Masaru Ohtsuru, Isao Tsuruo, Tadao Hata
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Effect of Heavy Metal Ions and Carbohydrates on the Activity of Cauliflower (Brassica oleracea Var. botrytis) Myrosinase [PDF]
Journal of Stress Physiology & Biochemistry, 2013 Myrosinase is an enzyme of cruciferous vegetables, hydrolyse glucosinolates. The breakdown products are involved in plant defence against insect and also have anti-fungal property.
Prakash, Om +3 more
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Glucosinolate Content in Dormant and Germinating Arabidopsis thaliana Seeds Is Affected by Non-Functional Alleles of Classical Myrosinase and Nitrile-Specifier Protein Genes [PDF]
Frontiers in Plant Science, 2019 While the defensive function of glucosinolates is well established, their possible role as a nutrient reservoir is poorly understood and glucosinolate turnover pathways have not been elucidated.
Kathrin Meier +2 more
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Screening, Identification and Enzymatic Properties of Myrosinase-Producing Sphingobacterium RJ35 [PDF]
Shipin Kexue, 2023 In this study, a myrosinase-producing strain named RJ35 was isolated from a rapeseed field soil, and it was identified by morphological and molecular biological methods.
ZHU Rongjun, FANG Yaowei, YE Qinwen, HOU Xiaoyue, YANG Guang, YANG Yunqiong, LIU Shu
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