A Simple Method for On-Gel Detection of Myrosinase Activity [PDF]
Molecules, 2018 Myrosinase is an enzyme present in many functional foods and spices, particularly in Cruciferous vegetables. It hydrolyses glucosinolates which thereafter rearrange into bioactive volatile constituents (isothiocyanates, nitriles).
Sándor Gonda +6 more
doaj +6 more sources
Studies on the Myrosinase in Mustard Seed [PDF]
Agricultural and Biological Chemistry, 1967 A characteristic effect of inorganic neutral salts on myrosinase was discovered. The salts containing monovalent anions had a remarkable inhibitory effect on the ascorbate-activated enzyme, but little on the non-activated enzyme. Such an effect was elucidated to be due to the anion.
Isao Tsuruo, Mizuho YOSHIDA, Tadao Hata
+13 more sources
Agricultural and Biological Chemistry, 1969 In order to obtain fungous myrosinase, Aspergillus sydowi IFO 4284 was cultured on a medium containing mustard seed extract for 2 weeks. Myrosinase in the broth was purified about 150 fold by precipitation with ammonium sulfate and chromatography on DEAE-cellulose and DEAE-Sephadex.
Masaru Ohtsuru, Isao Tsuruo, Tadao Hata
+8 more sources
Hijacking the Mustard-Oil Bomb: How a Glucosinolate-Sequestering Flea Beetle Copes With Plant Myrosinases [PDF]
Frontiers in Plant Science, 2021 Myrosinase enzymes play a key role in the chemical defense of plants of the order Brassicales. Upon herbivory, myrosinases hydrolyze the β-S-linked glucose moiety of glucosinolates, the characteristic secondary metabolites of brassicaceous plants, which ...
Theresa Sporer +6 more
doaj +3 more sources
Arabidopsis cDNA Sequence Encoding Myrosinase [PDF]
Plant Physiology, 1993 Myrosinase (0-thioglucosidase, thioglucoside glucohydrolase, EC 3.2.3.1) catalyzes the hydrolysis of glucosinolates, a class of sulfur-containing glycosides present in all Crucifers. Although intact glucosinolates are relatively nontoxic, their breakdown products (isothiocyanates, nitriles, or thiocyanates) have important biological influences on ...
Supachitra Chadchawan +5 more
openalex +7 more sources
Eradication of Myrosinase-Tethered Cancer Cells by Allyl Isothiocyanate Derived from Enzymatic Hydrolysis of Sinigrin [PDF]
Pharmaceutics, 2022 Sinigrin is present in significant amounts in cruciferous vegetables. Epidemiological studies suggest that the consumption of such vegetables decreases the risk of cancer, and the effect is attributed mainly to allyl isothiocyanate (AITC), a hydrolysis ...
Ammar Tarar +2 more
doaj +2 more sources
Microorganisms—An Effective Tool to Intensify the Utilization of Sulforaphane [PDF]
Foods, 2022 Sulforaphane (SFN) was generated by the hydrolysis of glucoraphanin under the action of myrosinase. However, due to the instability of SFN, the bioavailability of SFN was limited. Meanwhile, the gut flora obtained the ability to synthesize myrosinase and
Xiude Li +5 more
doaj +2 more sources
Myrosinase Compatible Simultaneous Determination of Glucosinolates and Allyl Isothiocyanate by Capillary Electrophoresis Micellar Electrokinetic Chromatography (CE‐MEKC) [PDF]
Phytochemical Analysis, 2016 Introduction. The functional food Cruciferous vegetables contain glucosinolates which are decomposed by the myrosinase enzyme upon tissue damage. The isothiocyanates are the most frequent decomposition products.
Sándor Gonda, Attila Kiss-szikszai
exaly +6 more sources
Molecular Docking of Potential Inhibitors of Broccoli Myrosinase [PDF]
Molecules, 2018 Glucosinolates are secondary metabolites occurring in Brassicaceae plants whose hydrolysis may yield isothiocyanates, widely recognized as health-promoting compounds. Myrosinase catalyzes this conversion.
J. Román, A. Castillo, A. Mahn
doaj +3 more sources
Supplementation of the Diet by Exogenous Myrosinase via Mustard Seeds to Increase the Bioavailability of Sulforaphane in Healthy Human Subjects after the Consumption of Cooked Broccoli [PDF]
Molecular Nutrition and Food Research, 2018 Broccoli contains the glucosinolate glucoraphanin which, in the presence of myrosinase, can hydrolyse to the isothiocyanate sulforaphane, reported to have anti-carcinogenic activity. However, the myrosinase enzyme is denatured on cooking.
Keshavan Niranjan +2 more
exaly +3 more sources