Results 21 to 30 of about 7,224 (201)
Immunological characterization of rapeseed myrosinase [PDF]
European Journal of Biochemistry, 1990 A purified 75‐kDa myrosinase and a crude rapeseed myrosinase fraction were used as antigens to produce mouse anti‐myrosinase monoclonal antibodies. The 75‐kDa myrosinase was also used to produce a polyclonal rabbit antiserum. The antiserum and one monoclonal antibody reacted with three distinct rapeseed polypeptides of 75, 70 and 65 kDa (M75, M70 and ...
M, Lenman +3 more
openaire +2 more sources
Distribution of Myrosinase in Rapeseed Tissues [PDF]
Plant Physiology, 1991 Immunocytochemical studies on Brassica napus (rapeseed) tissues using a monoclonal antibody against myrosinase (thioglucoside glucohydrolase) showed that the enzyme was only present in a small number of cells. In the developing embryo, scattered myrosinase-containing cells were present in both cotyledons and axis.
A S, Höglund +3 more
openaire +2 more sources
Foods, 2021 Glucosinolates are secondary plant metabolites present in Brassica vegetables. The endogenous enzyme myrosinase is responsible for the hydrolysis of glucosinolates, yielding a variety of compounds, including health-promoting isothiocyanates.
Omobolanle O. Oloyede +2 more
doaj +1 more source
Ultrasonics Sonochemistry, 2022 In this investigation, the combinations of exogenous pretreatment (melatonin or vitamin C) and contact ultrasound-assisted air drying were utilized to dry broccoli florets. To understand the influences of the studied dehydration methods on the conversion
Beini Liu +6 more
doaj +1 more source
Mechanistic studies on myrosinase [PDF]
, 2018 Myrosinase is the beta-thioglucosidase enzyme which catalyses the hydrolysis of glucosinolates, a group of naturally occurring plant metabolites. Glucosinolates are S-glucosides which occur predominantly in the family Cruciferae, and are abundant in the ...
Taylor, Malcolm G.
core +2 more sources
High-level and reusable preparation of sulforaphane by yeast cells expressing myrosinase
Food Chemistry: X, 2023 Myrosinase is a key tool for the fast and efficient preparation of sulforaphane which is one of the prominent natural ingredients found in brassicaceous vegetables.
Lili Wang +9 more
doaj +1 more source
A Simple Method for On-Gel Detection of Myrosinase Activity
Molecules, 2018 Myrosinase is an enzyme present in many functional foods and spices, particularly in Cruciferous vegetables. It hydrolyses glucosinolates which thereafter rearrange into bioactive volatile constituents (isothiocyanates, nitriles).
Sándor Gonda +6 more
doaj +1 more source
Broccoli Myrosinase cDNA Expression in Escherichia coli and Saccharomyces cerevisiae
Biomolecules, 2022 Myrosinases (EC 3.2.1.147) are enzymes known for the generation of hydrolysis products that have a potential beneficial effect on human health. Their reaction mechanisms are widely studied, in order to improve and optimize secondary metabolite production
Carolina Curiqueo +2 more
doaj +1 more source
Plant Defence Metabolite Detoxification by a Pathogenic Fungus Opens Necrotic Tissue to Diverse Bacteria in Arabidopsis thaliana. [PDF]
Environ Microbiol RepSclerotinia sclerotiorum, a devastating necrotrophic fungal pathogen of many crops, recruits a specific microbiome to necrotic, diseased leaf tissue. The interaction between plant formation and fungal degradation of aliphatic glucosinolate‐derived defence metabolites nearly completely controls this recruitment. ABSTRACT Foliar necrotic fungal pathogens
Sharma S, Chen J, Agler M.
europepmc +2 more sources
Inactivation of Thioglucosidase from Sinapis alba (White Mustard) Seed by Metal Salts
Molecules, 2020 The glucosinolates which are specialized plant metabolites of Brassica vegetables are prone to hydrolysis catalyzed by an endogenous enzyme myrosinase (thioglycoside hydrolase, thioglucosidase) that exists in Brassica plant tissue causing volatile ...
Monika Marcinkowska, Henryk H. Jeleń
doaj +1 more source