Results 11 to 20 of about 5,303 (173)
The study of Zn2+ and Cu2+ effects on myrosinase activity and sulforaphane production in Lepidium draba [PDF]
Myrosinase is a β-thioglucoside glucohydrolase enzyme which catalyzses the separation of glucose from glucosinolates and produces an unstable intermediate, aglycone.
Mehdi Mohammadi +3 more
doaj +1 more source
Distribution of Myrosinase in Rapeseed Tissues [PDF]
Immunocytochemical studies on Brassica napus (rapeseed) tissues using a monoclonal antibody against myrosinase (thioglucoside glucohydrolase) showed that the enzyme was only present in a small number of cells. In the developing embryo, scattered myrosinase-containing cells were present in both cotyledons and axis.
A S, Höglund +3 more
openaire +2 more sources
Extended darkness induces internal turnover of glucosinolates in Arabidopsis thaliana leaves. [PDF]
Prolonged darkness leads to carbohydrate starvation, and as a consequence plants degrade proteins and lipids to oxidize amino acids and fatty acids as alternative substrates for mitochondrial ATP production.
Saskia Brandt +5 more
doaj +1 more source
Yellow mustards are widely cultivated for their spicy flavor and are rich source of glucosinolates and myrosinase. The predominant glucosinolate in yellow mustard seeds is sinalbin and corresponding isothiocyanate is 4-hydroxybenzyl isothiocyanate upon ...
Zhitao Pan +6 more
doaj +1 more source
Sinigrin is present in significant amounts in cruciferous vegetables. Epidemiological studies suggest that the consumption of such vegetables decreases the risk of cancer, and the effect is attributed mainly to allyl isothiocyanate (AITC), a hydrolysis ...
Ammar Tarar +2 more
doaj +1 more source
Immunological characterization of rapeseed myrosinase [PDF]
A purified 75‐kDa myrosinase and a crude rapeseed myrosinase fraction were used as antigens to produce mouse anti‐myrosinase monoclonal antibodies. The 75‐kDa myrosinase was also used to produce a polyclonal rabbit antiserum. The antiserum and one monoclonal antibody reacted with three distinct rapeseed polypeptides of 75, 70 and 65 kDa (M75, M70 and ...
M, Lenman +3 more
openaire +2 more sources
Glucosinolates are secondary plant metabolites present in Brassica vegetables. The endogenous enzyme myrosinase is responsible for the hydrolysis of glucosinolates, yielding a variety of compounds, including health-promoting isothiocyanates.
Omobolanle O. Oloyede +2 more
doaj +1 more source
In this investigation, the combinations of exogenous pretreatment (melatonin or vitamin C) and contact ultrasound-assisted air drying were utilized to dry broccoli florets. To understand the influences of the studied dehydration methods on the conversion
Beini Liu +6 more
doaj +1 more source
Broccoli Myrosinase cDNA Expression in Escherichia coli and Saccharomyces cerevisiae
Myrosinases (EC 3.2.1.147) are enzymes known for the generation of hydrolysis products that have a potential beneficial effect on human health. Their reaction mechanisms are widely studied, in order to improve and optimize secondary metabolite production
Carolina Curiqueo +2 more
doaj +1 more source
A Simple Method for On-Gel Detection of Myrosinase Activity
Myrosinase is an enzyme present in many functional foods and spices, particularly in Cruciferous vegetables. It hydrolyses glucosinolates which thereafter rearrange into bioactive volatile constituents (isothiocyanates, nitriles).
Sándor Gonda +6 more
doaj +1 more source

