Results 11 to 20 of about 5,303 (173)

The study of Zn2+ and Cu2+ effects on myrosinase activity and sulforaphane production in Lepidium draba [PDF]

open access: yesمجله بیوتکنولوژی کشاورزی, 2016
Myrosinase is a β-thioglucoside glucohydrolase enzyme which catalyzses the separation of glucose from glucosinolates and produces an unstable intermediate, aglycone.
Mehdi Mohammadi   +3 more
doaj   +1 more source

Distribution of Myrosinase in Rapeseed Tissues [PDF]

open access: yesPlant Physiology, 1991
Immunocytochemical studies on Brassica napus (rapeseed) tissues using a monoclonal antibody against myrosinase (thioglucoside glucohydrolase) showed that the enzyme was only present in a small number of cells. In the developing embryo, scattered myrosinase-containing cells were present in both cotyledons and axis.
A S, Höglund   +3 more
openaire   +2 more sources

Extended darkness induces internal turnover of glucosinolates in Arabidopsis thaliana leaves. [PDF]

open access: yesPLoS ONE, 2018
Prolonged darkness leads to carbohydrate starvation, and as a consequence plants degrade proteins and lipids to oxidize amino acids and fatty acids as alternative substrates for mitochondrial ATP production.
Saskia Brandt   +5 more
doaj   +1 more source

Optimization the conversion of glucosinolate to isothiocyanate in yellow mustard seeds (Sinapis alba) by response surface methodology

open access: yesApplied Food Research, 2022
Yellow mustards are widely cultivated for their spicy flavor and are rich source of glucosinolates and myrosinase. The predominant glucosinolate in yellow mustard seeds is sinalbin and corresponding isothiocyanate is 4-hydroxybenzyl isothiocyanate upon ...
Zhitao Pan   +6 more
doaj   +1 more source

Eradication of Myrosinase-Tethered Cancer Cells by Allyl Isothiocyanate Derived from Enzymatic Hydrolysis of Sinigrin

open access: yesPharmaceutics, 2022
Sinigrin is present in significant amounts in cruciferous vegetables. Epidemiological studies suggest that the consumption of such vegetables decreases the risk of cancer, and the effect is attributed mainly to allyl isothiocyanate (AITC), a hydrolysis ...
Ammar Tarar   +2 more
doaj   +1 more source

Immunological characterization of rapeseed myrosinase [PDF]

open access: yesEuropean Journal of Biochemistry, 1990
A purified 75‐kDa myrosinase and a crude rapeseed myrosinase fraction were used as antigens to produce mouse anti‐myrosinase monoclonal antibodies. The 75‐kDa myrosinase was also used to produce a polyclonal rabbit antiserum. The antiserum and one monoclonal antibody reacted with three distinct rapeseed polypeptides of 75, 70 and 65 kDa (M75, M70 and ...
M, Lenman   +3 more
openaire   +2 more sources

Influence of Cabbage (Brassica oleracea) Accession and Growing Conditions on Myrosinase Activity, Glucosinolates and Their Hydrolysis Products

open access: yesFoods, 2021
Glucosinolates are secondary plant metabolites present in Brassica vegetables. The endogenous enzyme myrosinase is responsible for the hydrolysis of glucosinolates, yielding a variety of compounds, including health-promoting isothiocyanates.
Omobolanle O. Oloyede   +2 more
doaj   +1 more source

Influence of sequential exogenous pretreatment and contact ultrasound-assisted air drying on the metabolic pathway of glucoraphanin in broccoli florets

open access: yesUltrasonics Sonochemistry, 2022
In this investigation, the combinations of exogenous pretreatment (melatonin or vitamin C) and contact ultrasound-assisted air drying were utilized to dry broccoli florets. To understand the influences of the studied dehydration methods on the conversion
Beini Liu   +6 more
doaj   +1 more source

Broccoli Myrosinase cDNA Expression in Escherichia coli and Saccharomyces cerevisiae

open access: yesBiomolecules, 2022
Myrosinases (EC 3.2.1.147) are enzymes known for the generation of hydrolysis products that have a potential beneficial effect on human health. Their reaction mechanisms are widely studied, in order to improve and optimize secondary metabolite production
Carolina Curiqueo   +2 more
doaj   +1 more source

A Simple Method for On-Gel Detection of Myrosinase Activity

open access: yesMolecules, 2018
Myrosinase is an enzyme present in many functional foods and spices, particularly in Cruciferous vegetables. It hydrolyses glucosinolates which thereafter rearrange into bioactive volatile constituents (isothiocyanates, nitriles).
Sándor Gonda   +6 more
doaj   +1 more source

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