Results 21 to 30 of about 5,678 (206)
Immunological characterization of rapeseed myrosinase [PDF]
European Journal of Biochemistry, 1990 A purified 75‐kDa myrosinase and a crude rapeseed myrosinase fraction were used as antigens to produce mouse anti‐myrosinase monoclonal antibodies. The 75‐kDa myrosinase was also used to produce a polyclonal rabbit antiserum. The antiserum and one monoclonal antibody reacted with three distinct rapeseed polypeptides of 75, 70 and 65 kDa (M75, M70 and ...
M, Lenman +3 more
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Foods, 2021 Glucosinolates are secondary plant metabolites present in Brassica vegetables. The endogenous enzyme myrosinase is responsible for the hydrolysis of glucosinolates, yielding a variety of compounds, including health-promoting isothiocyanates.
Omobolanle O. Oloyede +2 more
doaj +1 more source
Ultrasonics Sonochemistry, 2022 In this investigation, the combinations of exogenous pretreatment (melatonin or vitamin C) and contact ultrasound-assisted air drying were utilized to dry broccoli florets. To understand the influences of the studied dehydration methods on the conversion
Beini Liu +6 more
doaj +1 more source
High-level and reusable preparation of sulforaphane by yeast cells expressing myrosinase
Food Chemistry: X, 2023 Myrosinase is a key tool for the fast and efficient preparation of sulforaphane which is one of the prominent natural ingredients found in brassicaceous vegetables.
Lili Wang +9 more
doaj +1 more source
A Simple Method for On-Gel Detection of Myrosinase Activity
Molecules, 2018 Myrosinase is an enzyme present in many functional foods and spices, particularly in Cruciferous vegetables. It hydrolyses glucosinolates which thereafter rearrange into bioactive volatile constituents (isothiocyanates, nitriles).
Sándor Gonda +6 more
doaj +1 more source
Inactivation of Thioglucosidase from Sinapis alba (White Mustard) Seed by Metal Salts
Molecules, 2020 The glucosinolates which are specialized plant metabolites of Brassica vegetables are prone to hydrolysis catalyzed by an endogenous enzyme myrosinase (thioglycoside hydrolase, thioglucosidase) that exists in Brassica plant tissue causing volatile ...
Monika Marcinkowska, Henryk H. Jeleń
doaj +1 more source
Factors Influencing Sulforaphane Content in Broccoli Sprouts and Subsequent Sulforaphane Extraction
Foods, 2021 Broccoli sprouts contain 10–100 times higher levels of sulforaphane than mature plants, something that has been well known since 1997. Sulforaphane has a whole range of unique biological properties, and it is especially an inducer of phase 2 detoxication
Jan Tříska +10 more
doaj +1 more source
Molecular Modelling of Broccoli Myrosinase and its Interaction with Different Ligands
Chemical Engineering Transactions, 2018 Myrosinase (ß-thioglucosidase glucohydrolase (EC 3.2.3.1)) is a glycoprotein responsible for the hydrolysis of glucosinolates, a group of sulfur-containing secondary metabolites present in Brassicaceae plants.
J. Roman +3 more
doaj +1 more source
Shipin gongye ke-ji, 2022 In order to understand the effect of air-borne ultrasound on the catabolic pathway of glucosinolates in broccoli florets during air drying, the drying property, contents of key components (glucosinolates, sulforaphane, ascorbic acid, Fe2+ and phenolic ...
Beini LIU +5 more
doaj +1 more source
Distribution of Myrosinase in Rapeseed Tissues [PDF]
Plant Physiology, 1991 Immunocytochemical studies on Brassica napus (rapeseed) tissues using a monoclonal antibody against myrosinase (thioglucoside glucohydrolase) showed that the enzyme was only present in a small number of cells. In the developing embryo, scattered myrosinase-containing cells were present in both cotyledons and axis.
A S, Höglund +3 more
openaire +2 more sources