Catalysts, 2022 The myrosinase enzyme hydrolyzes glucosinolates, among which is glucoraphanin, the precursor of the anticancer isothiocyanate sulforaphane (SFN). The main source of glucoraphanin is Brassicaceae; however, its natural concentration is relatively low ...
A. Jiménez, Antonio Castillo, A. Mahn
semanticscholar +1 more source
Myrosinase Compatible Simultaneous Determination of Glucosinolates and Allyl Isothiocyanate by Capillary Electrophoresis Micellar Electrokinetic Chromatography (CE-MEKC) [PDF]
, 2016 Introduction. The functional food Cruciferous vegetables contain glucosinolates which are decomposed by the myrosinase enzyme upon tissue damage. The isothiocyanates are the most frequent decomposition products.
Gonda, Sándor +4 more
core +4 more sources
High-level and reusable preparation of sulforaphane by yeast cells expressing myrosinase
Food Chemistry: X, 2023 Myrosinase is a key tool for the fast and efficient preparation of sulforaphane which is one of the prominent natural ingredients found in brassicaceous vegetables.
Lili Wang +9 more
doaj +1 more source
Aryl hydrocarbon receptor activation during in vitro and in vivo digestion of raw and cooked broccoli (brassica oleracea var. Italica) [PDF]
, 2020 Broccoli is rich in glucosinolates, which can be converted upon chewing and processing into Aryl hydrocarbon Receptor (AhR) ligands. Activation of AhR plays an important role in overall gut homeostasis but the role of broccoli processing on the ...
Capuano, Edoardo +11 more
core +3 more sources
Micellar electrokinetic capillary chromatography—Synchronous monitoring of substrate and products in the myrosinase catalysed hydrolysis of glucosinolates [PDF]
, 2006 A micellar electrokinetic capillary chromatography (MECC) method has been developed for monitoring the myrosinase catalysed hydrolysis of 2-hydroxy substituted glucosinolates and the simultaneous formation of the corresponding degradation products ...
Bellostas, Natalia +2 more
core +1 more source
Inactivation of Thioglucosidase from Sinapis alba (White Mustard) Seed by Metal Salts
Molecules, 2020 The glucosinolates which are specialized plant metabolites of Brassica vegetables are prone to hydrolysis catalyzed by an endogenous enzyme myrosinase (thioglycoside hydrolase, thioglucosidase) that exists in Brassica plant tissue causing volatile ...
Monika Marcinkowska, Henryk H. Jeleń
doaj +1 more source
Factors Influencing Sulforaphane Content in Broccoli Sprouts and Subsequent Sulforaphane Extraction
Foods, 2021 Broccoli sprouts contain 10–100 times higher levels of sulforaphane than mature plants, something that has been well known since 1997. Sulforaphane has a whole range of unique biological properties, and it is especially an inducer of phase 2 detoxication
Jan Tříska +10 more
doaj +1 more source
Effect of stevia supplementation of kale juice spheres on their quality changes during refrigerated shelf life [PDF]
, 2018 Kale is a vegetable with high contents of health–promoting compounds although its consumption as a beverage is highly limited due to its bitter flavour. Nonetheless, the bitter flavour of Brassicas may be masked by sweetening.
Artés Calero, Francisco +4 more
core +2 more sources
Molecular Modelling of Broccoli Myrosinase and its Interaction with Different Ligands
Chemical Engineering Transactions, 2018 Myrosinase (ß-thioglucosidase glucohydrolase (EC 3.2.3.1)) is a glycoprotein responsible for the hydrolysis of glucosinolates, a group of sulfur-containing secondary metabolites present in Brassicaceae plants.
J. Roman +3 more
doaj +1 more source
Shipin gongye ke-ji, 2022 In order to understand the effect of air-borne ultrasound on the catabolic pathway of glucosinolates in broccoli florets during air drying, the drying property, contents of key components (glucosinolates, sulforaphane, ascorbic acid, Fe2+ and phenolic ...
Beini LIU +5 more
doaj +1 more source