Results 61 to 70 of about 5,303 (173)
Studies on the Myrosinase fromWasabia japonica:Purification and Some Properties of Wasabi Myrosinase
The myrosinase from Wasabi (Wasabia japonica) is effectively extracted by sonication. The enzyme is purified about 100-fold by gel-filtration on Sephacryl S-200, Sepharose 6B and ion-exchange chromatography on DEAE-Sephadex. The enzyme is active only when L-ascorbic acid is added to the reaction mixture. General reducing reagents (2-mercaptoethanol and
OHTSURU, Masaru, KAWATANI, Harumi
openaire +2 more sources
Through HPLC–MS/MS analysis and multivariate statistics, raphani semen (RS) varieties with high functional components were screened. The white raphani semen “Xuebawang” was identified as ideal for further study due to its high bioactive content: glucoraphenin (9010.27 mg/100 g), glucoraphanin (1027.76 mg/100 g), sulforaphane (66.14 mg/100 g), and ...
Yonghua Li +10 more
wiley +1 more source
Restoration of Immune Surveillance in Prostate Cancer Prevention by Sulforaphane in Hi‐Myc Mice
ABSTRACT Oral administration of broccoli constituent sulforaphane (SFN) prevents prostate cancer development in preclinical mouse models. However, the mechanism(s) underlying prostate cancer prevention by SFN are not fully understood. In this study, we used a human relevant mouse model (Hi‐Myc mice) to demonstrate restoration of immune surveillance by ...
Krishna B. Singh +3 more
wiley +1 more source
Bioavailability of glucosinolates and their breakdown products: Impact of processing
Glucosinolates are a large group of plant secondary metabolites with nutritional effects, and are mainly found in cruciferous plants. After ingestion, glucosinolates could be partially absorbed in their intact form through the gastrointestinal mucosa ...
Francisco Jose Barba +6 more
doaj +1 more source
Phytochemical diversity is thought to result from coevolutionary cycles as specialization in herbivores imposes diversifying selection on plant chemical defenses.
Tobias Züst +16 more
doaj +1 more source
ESCRT: A Cellular Nexus of Plant Immunity and Pathogen Counter‐Strategies
The endosomal sorting complex required for transport (ESCRT) plays multifaceted roles in plants' responses to pathogens, including regulation of the localization and fate of immune receptors and other related cellular processes. ABSTRACT The endosomal sorting complex required for transport (ESCRT) machinery consists of multiprotein complexes conserved ...
Willam Kyle Sexton, Shunyuan Xiao
wiley +1 more source
Abstract Biofumigation with brassica cover crops (BCCs) is gaining momentum in the United States as an ecologically sound alternative to chemical pest suppression in agricultural systems. This review critically evaluates the impacts of Brassica species on soil health, weed suppression, disease mitigation, and control of plant‐parasitic nematodes (PPNs)
Karthik Karuppanan +4 more
wiley +1 more source
Flavodiiron proteins (FDPs) are di‐iron enzymes that reduce NO and/or O2 with distinct substrate selectivity. We have produced E. coli FDP variants targeting second coordination‐sphere residues and determined their structures. Although the kinetics remained unchanged, the E.
Patrícia T. Borges +8 more
wiley +1 more source
Studies on the Myrosinase in Mustard Seed [PDF]
In order to clarify whether the myrosinase containes only thioglucosidase, or thioglucosidase and sulfatase, chromatographic behaviors of the myrosinase on cellulose ion exchanger were studied. Myrosinase showed two peaks by the chromatography on TEAE-cellulose at pH 8.5, but the fractionation of the two enzymes was not accomplished.
Isao Tsuruo, Mizuho Yoshida, Tadao Hata
openaire +1 more source
Gap Analysis of Metabolic Conversions of Off‐Flavors and Antinutrients in Plant‐Based Substrates
ABSTRACT To drastically reduce the carbon footprint of the food production chain, a major shift towards alternatives to conventional meat and dairy products is required. The use of plant‐based proteins is a promising route, but it also comes with challenges: Plant‐based proteins often contain antinutritional factors and off‐flavors, which can ...
Robin I. Kuijpers +16 more
wiley +1 more source

