Results 61 to 70 of about 95,722 (299)

RESOLVING EXTENDED N‐GLYCANS BY NMR: NEW INSIGHTS INTO INFLUENZA HEMAGGLUTININ N‐GLYCAN INTERACTIONS

The FASEB Journal, 2018
Seasonal flu (influenza virus infection) is one of the major threats to human health, causing of up to 500 thousands deaths worldwide annually.1,2 Hemagglutinin, a glycoprotein presented in the surface of the influenza virus, binds sialylated N‐glycans located on the surface of host epithelial cells. The structural elucidation and the
Beatriz Fernández de Toro Ronda, Wenjie Peng, Andrew Thompson, Gema Domínguez, F. Javier Cañada, Javier Pérez Castells, Jesús Jiménez Barbero, James C. Paulson, Ángeles Canales   +8 more
openaire   +1 more source

A Chemoenzymatic Method for Glycoproteomic N-glycan Type Quantitation [PDF]

Analytical Chemistry, 2019
ABSTRACTGlycosylation is one of the most important post-translational modifications in biological systems. Current glycoproteome methods mainly focus on qualitative identification of glycosylation sites or intact glycopeptides. However, the systematic quantitation of glycoproteins has remained largely unexplored.
Henghui Li, Leyuan Li, Kai Cheng, Zhibin Ning, Janice Mayne, Xu Zhang, Krystal Walker, Rui Chen, Susan Twine, Jianjun Li, Daniel Figeys   +10 more
openaire   +3 more sources

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

The Association Between Normal BMI With Central Adiposity And Proinflammatory Potential Immunoglobulin G N-Glycosylation

Diabetes, Metabolic Syndrome and Obesity, 2019
Di Liu,1,* Qihuan Li,1,2,* Jing Dong,3 Dong Li,4 Xizhu Xu,4 Weijia Xing,4 Xiaoyu Zhang,1 Weijie Cao,1 Haifeng Hou,4 Hao Wang,1,5 Manshu Song,1 Lixin Tao,1 Xiaoping Kang,6 Qun Meng,1 Wei Wang,1,4,5 Xiuhua Guo,1 Youxin Wang1 1Beijing Key Laboratory of ...
Liu D, Li Q, Dong J, Li D, Xu X, Xing W, Zhang X, Cao W, Hou H, Wang H, Song M, Tao L, Kang X, Meng Q, Wang W, Guo X, Wang Y   +16 more
doaj  

Plasma High-Mannose and Complex/Hybrid N-Glycans Are Associated with Hypercholesterolemia in Humans and Rabbits.

PLoS ONE, 2016
N-glycans play important roles in various pathophysiological processes and can be used as clinical diagnosis markers. However, plasma N-glycans change and their pathophysiological significance in the setting of hypercholesterolemia, a major risk factor ...
Liang Bai, Qianwei Li, Lingmei Li, Yan Lin, Sihai Zhao, Weirong Wang, Rong Wang, Yongqin Li, Jiangbei Yuan, Chengjian Wang, Zhongfu Wang, Jianglin Fan, Enqi Liu   +12 more
doaj   +1 more source

Glyco-Decipher enables glycan database-independent peptide matching and in-depth characterization of site-specific N-glycosylation

Nature Communications, 2022
Poor peptide fragmentation and unusual glycan structures limit mass spectrometry-based analysis of intact N-glycopeptides. Here, the authors develop Glyco-Decipher, a glycan-independent peptide search tool, to tackle these issues and improve the coverage
Zheng Fang, Hongqiang Qin, Jiawei Mao, Zhongyu Wang, Na Zhang, Yan Wang, Luyao Liu, Yongzhan Nie, Mingming Dong, Mingliang Ye   +9 more
doaj   +1 more source

Serum Linkage-Specific Sialylation Changes Are Potential Biomarkers for Monitoring and Predicting the Recurrence of Papillary Thyroid Cancer Following Thyroidectomy

Frontiers in Endocrinology, 2022
BackgroundAlthough papillary thyroid cancer (PTC) could remain indolent, the recurrence rates after thyroidectomy are approximately 20%. There are currently no accurate serum biomarkers that can monitor and predict recurrence of PTC after thyroidectomy ...
Zhen Cao, Zejian Zhang, Rui Liu, Mengwei Wu, Zepeng Li, Xiequn Xu, Ziwen Liu   +6 more
doaj   +1 more source

A structural and functional model for human bone sialoprotein [PDF]

, 2012
Human bone sialoprotein (BSP) is an essential component of the extracellular matrix of bone. It is thought to be the primary nucleator of hydroxyapatite crystallization, and is known to bind to hydroxyapatite, collagen, and cells.
Durrant, Marcus, Vincent, Kevin
core   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Rapid chemical de-N-glycosylation and derivatization for liquid chromatography of immunoglobulin N-linked glycans. [PDF]

PLoS ONE, 2018
Glycan analysis may result in exploitation of glycan biomarkers and evaluation of heterogeneity of glycosylation of biopharmaceuticals. For N-linked glycan analysis, we investigated alkaline hydrolysis of the asparagine glycosyl carboxamide of ...
Akihiko Kameyama, Santha Kumara Dissanayake, Wai Wai Thet Tin   +2 more
doaj   +1 more source