Concanavalin A-Nonbinding Enzymes of Crotalus scutulatus scutulatus Venom [PDF]
Crotalus scutulatus scutulatus crude venom was separated into two fractions by Concanavalin A Sepharose 4B affinity chromatography. The Concanavalin A-nonbinding fraction (F-l) exhibited phosphomonoesterase (orthophosphoric monoester phosphohydrolase EC ...
Childs, C. K.+3 more
core +6 more sources
Nucleosided derived antibiotics to fight microbial drug resistance: New utilities for an established class of drugs? [PDF]
Novel antibiotics are urgently needed to combat the rise of infections due to drug-resistant microorganisms. Numerous natural nucleosides and their synthetically modified analogues have been reported to have moderate to good antibiotic activity against ...
Ferrari, Valentina+2 more
core +1 more source
Isoniazid-resistant mutants of Mycobacterium tuberculosis H 37 RV: uptake of isoniazid and the properties of NADase inhibitor [PDF]
Six independent isoniazid-resistant mutants of Mycobacterium tuberculosis were isolated under conditions which largely ensured the selection of one-step mutants.
Ramakrishnan, T., Sriprakash, K. S.
core +1 more source
The physico-chomical and enzymatic properties of NAD (P) nucelsidase from skipjack liver was examined using a partially purified and two purified specimens (Frs. I and II). The molecular weights of Fr. I and II enzymes were estimated to be 30, 000 and 20, 000 and the isoelectric points to be 3.5 and 4.3, respectively. On SDS-electeophoresis, the Fr. II
Chiaki Shimizu+2 more
openaire +3 more sources
Quantitative proteomic analysis of the influence of lignin on biofuel production by Clostridium acetobutylicum ATCC 824 [PDF]
Background: Clostridium acetobutylicum has been a focus of research because of its ability to produce high-value compounds that can be used as biofuels.
AM López-Contreras+76 more
core +5 more sources
Arabidopsis thaliana nucleosidase mutants provide new insights into nucleoside degradation [PDF]
A central step in nucleoside and nucleobase salvage pathways is the hydrolysis of nucleosides to their respective nucleobases. In plants this is solely accomplished by nucleosidases (EC 3.2.2.x).To elucidate the importance of nucleosidases for nucleoside
Geserick, Claudia+2 more
core +2 more sources
S‐Adenosylmethionine‐dependent methyltransferases are emerging enzymes for selective biocatalytic alkylation. Introduction of the direct sulfurylation pathway from S. cerevisiae improved methylation up to 17‐fold, allowing straight‐forward isolation of the methylated products.
Michael K. F. Mohr+2 more
wiley +2 more sources
Utilization of tryptophan, nicotinamide and nicotinic acid as precursors for nicotinamide nucleotide synthesis in isolated rat liver cells [PDF]
1. Incubation of isolated rat hepatocytes with nicotinamide or nicotinic acid showed that while both vitamers were taken up from the incubation medium, neither was utilized to any significant extent as a precursor of the nicotinamide nucleotide coenzymes,
Bender, DA, Olufunwa, R
core +1 more source
Structural and Biochemical Characterization of Chlamydia trachomatis Hypothetical Protein CT263 Supports That Menaquinone Synthesis Occurs through the Futalosine Pathway [PDF]
BACKGROUND: Specific pathways and components for respiration in Chlamydia are poorly understood. RESULTS: The C. trachomatis hypothetical protein CT263 crystal structure displays strong structural similarity with 5′-methylthioadenosine nucleosidase ...
Barta, Michael L.+6 more
core +1 more source
Construction of microbial platform for an energy-requiring bioprocess: practical 2′-deoxyribonucleoside production involving a C−C coupling reaction with high energy substrates [PDF]
BACKGROUND: Reproduction and sustainability are important for future society, and bioprocesses are one technology that can be used to realize these concepts.
Jun Ogawa+8 more
core +1 more source