Results 81 to 90 of about 11,490,346 (250)

Electrocatalytic NAD(P)H regeneration for biosynthesis

Green Chemical Engineering
The highly efficient chemoselectivity, stereoselectivity, and regioselectivity render enzyme catalysis an ideal pathway for the synthesis of various chemicals in broad applications.
Yaoxuan Li, Guanhua Liu, Weixi Kong, Suoqing Zhang, Yuemei Bao, Hao Zhao, Lihui Wang, Liya Zhou, Yanjun Jiang   +8 more
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Untreated organoids contain cells with high levels of NAD(P)H intensity and cells with low levels of NAD(P)H intensity.

, 2017
(A) A representative fluorescence intensity image shows organoids with two levels of NAD(P)H intensity. (B) Low NAD(P)H cells exhibit a lower optical redox ratio than high NAD(P)H cells.
Melissa C. Skala (530309), Amy T. Shah (530305), Tiffany M. Heaster (3666343)   +2 more
core   +1 more source

Cytosolic Redox Status of Wine Yeast (Saccharomyces Cerevisiae) under Hyperosmotic Stress during Icewine Fermentation

Fermentation, 2017
Acetic acid is undesired in Icewine. It is unclear whether its production by fermenting yeast is linked to the nicotinamide adenine dinucleotide (NAD+/NADH) system or the nicotinamide adenine dinucleotide phosphate (NADP+/NADPH) system.
Fei Yang, Caitlin Heit, Debra L. Inglis
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

ZINC-DEFICIENCY ENHANCED NAD(P)H-DEPENDENT SUPEROXIDE RADICAL PRODUCTION IN PLASMA-MEMBRANE VESICLES ISOLATED FROM ROOTS OF BEAN-PLANTS

, 1994
The production of superoxide radical (O-2(.-)) was studied in plasma membrane vesicles isolated by aqueous polymer two-phase partitioning from roots of zinc-sufficient and zinc-deficient bean (Phaseolus vulgaris L. cv. Prelude) plants.
Pinton R., PINTON, Roberto, MARSCHNER H., Cakmak I., CAKMAK I   +4 more
core   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Identification of a magnesium-dependent NAD(P)(H)-binding domain in the nicotinoprotein methanol dehydrogenase from Bacillus methanolicus [PDF]

, 2002
The Bacillus methanolicus methanol dehydrogenase (MDH) is a decameric nicotinoprotein alcohol dehydrogenase (family III) with one Zn2+ ion, one or two Mg2+ ions, and a tightly bound cofactor NAD(H) per subunit.
Kloosterman, H, Hektor, HJ, Dijkhuizen, L; id_orcid, Dijkhuizen, L   +3 more
core   +2 more sources

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Proposed mechanism of FMN-DI catalyzing the hydride transfer between NAD(H) and NADP(H).

, 2016
Proposed mechanism of FMN-DI catalyzing the hydride transfer between NAD(H) and NADP(H).
Fangfang Sun (2410654), Ting Zhang (102583), Jinglin Fu (225092), John Collins (1769020), Scott Huston (2657011), Y.-H. Percival Zhang (28712)   +5 more
core   +1 more source