Results 1 to 10 of about 268,882 (295)

TCA cycle tailoring facilitates optimal growth of proton-pumping NADH dehydrogenase-dependent Escherichia coli [PDF]

Microbiology Spectrum, 2023
The bacterial lifestyle is plastic, requiring transcriptional, translational, and metabolic tailoring for survival. These dynamic cellular processes are energy intensive; therefore, flexible energetics is requisite for adaptive plasticity.
Nikita Goel, Stuti Srivastav, Arjun Patel, Akshay Shirsath, Tushar Ranjan Panda, Malay Patra, Adam M. Feist, Amitesh Anand   +7 more
doaj   +3 more sources

Relevance of NADH Dehydrogenase and Alternative Two-Enzyme Systems for Growth of Corynebacterium glutamicum With Glucose, Lactate, and Acetate [PDF]

Frontiers in Bioengineering and Biotechnology, 2021
The oxidation of NADH with the concomitant reduction of a quinone is a crucial step in the metabolism of respiring cells. In this study, we analyzed the relevance of three different NADH oxidation systems in the actinobacterial model organism ...
Tomoya Maeda, Abigail Koch-Koerfges, Michael Bott   +2 more
doaj   +2 more sources

Two-Photon Excited Fluorescence of NADH-Alcohol Dehydrogenase Complex in a Mixture with Bacterial Enzymes

Biomolecules, 2023
Thorough study of composition and fluorescence properties of a commercial reagent of active equine NAD-dependent alcohol dehydrogenase expressed and purified from E. coli has been carried out. Several experimental methods: spectral- and time-resolved two-
Ioanna A. Gorbunova, Maxim E. Sasin, Dmitry V. Yachkov, Denis A. Volkov, Alexei D. Vedyaykin, Andrey A. Nikiforov, Oleg S. Vasyutinskii   +6 more
doaj   +2 more sources

NADH Dehydrogenase of Corynebacterium glutamicum. Purification of an NADH Dehydrogenase II Homolog Able to Oxidize NADPH. [PDF]

FEMS Microbiology Letters, 2001
NADPH oxidase activity, in addition to NADH oxidase activity, has been shown to be present in the respiratory chain of Corynebacterium glutamicum. In this study, we tried to purify NADPH oxidase and NADH dehydrogenase activities from the membranes of C. glutamicum.
Hirohide Toyama, Kazunobu Matsushita, M Iwahashi   +2 more
exaly   +4 more sources

Differences in mitochondrial NADH dehydrogenase activities in trypanosomatids. [PDF]

Parasitology, 2021
AbstractComplex I (NADH dehydrogenase) is the first enzyme in the respiratory chain. It catalyses the electron transfer from NADH to ubiquinone that is associated with proton pumping out of the matrix. In this study, we characterized NADH dehydrogenase activity in seven monoxenous trypanosomatid species: Blechomonas ayalai, Herpetomonas tarakana ...
Čermáková P, Maďarová A, Baráth P, Bellová J, Yurchenko V, Horváth A.   +5 more
europepmc   +4 more sources

A Bacillales-specific tubular scaffold essential for NADH dehydrogenase activity [PDF]

Nature Communications
Respiratory type II NADH:quinone oxidoreductases (NDH-II) are typically monotopic flavoproteins that make direct contact with the membrane to access the quinone pool.
Farida Seduk, Rayan Osman, Pierre Simon Garcia, Lilou Bizien-Jaglin, Pauline Juyoux, Artemis Kosta, Salomé Sauvage, Maria J. Maté, Fabien Pierrel, Régine Lebrun, Guy Schoehn, Yoshiki Yamaryo-Botté, Cyrille Y. Botté, Yvain Nicolet, Mickael V. Cherrier, Anne Walburger, Axel Magalon   +16 more
doaj   +2 more sources

Synthetic lethality of Mycobacterium tuberculosis NADH dehydrogenases is due to impaired NADH oxidation

mBio, 2023
Type 2 NADH dehydrogenase (Ndh-2) is an oxidative phosphorylation enzyme discussed as a promising drug target in different pathogens, including Plasmodium falciparum and Mycobacterium tuberculosis (Mtb).
Yuanyuan Xu, Sabine Ehrt, Dirk Schnappinger, Tiago Beites   +3 more
doaj   +1 more source

Structural and functional insights into (S)-ureidoglycolate dehydrogenase, a metabolic branch point enzyme in nitrogen utilization. [PDF]

PLoS ONE, 2012
Nitrogen metabolism is one of essential processes in living organisms. The catabolic pathways of nitrogenous compounds play a pivotal role in the storage and recovery of nitrogen. In Escherichia coli, two different, interconnecting metabolic routes drive
Myung-Il Kim, Inchul Shin, Suhee Cho, Jeehyun Lee, Sangkee Rhee   +4 more
doaj   +1 more source

Metabolic Engineering of Enterobacter aerogenes for Improved 2,3-Butanediol Production by Manipulating NADH Levels and Overexpressing the Small RNA RyhB

Frontiers in Microbiology, 2021
Biotechnological production of 2,3-butanediol (2,3-BD), a versatile platform bio-chemical and a potential biofuel, is limited due to by-product toxicity.
Yan Wu, Yan Wu, Wanying Chu, Jiayao Yang, Yudong Xu, Qi Shen, Haoning Yang, Fangxu Xu, Yefei Liu, Ping Lu, Ke Jiang, Hongxin Zhao   +11 more
doaj   +1 more source

Yeast NDI1 reconfigures neuronal metabolism and prevents the unfolded protein response in mitochondrial complex I deficiency.

PLoS Genetics, 2023
Mutations in subunits of the mitochondrial NADH dehydrogenase cause mitochondrial complex I deficiency, a group of severe neurological diseases that can result in death in infancy. The pathogenesis of complex I deficiency remain poorly understood, and as
Lucy Granat, Debbra Y Knorr, Daniel C Ranson, Emma L Hamer, Ram Prosad Chakrabarty, Francesca Mattedi, Laura Fort-Aznar, Frank Hirth, Sean T Sweeney, Alessio Vagnoni, Navdeep S Chandel, Joseph M Bateman   +11 more
doaj   +1 more source