Results 81 to 90 of about 465,834 (342)

The putative isomerization of the lactate dehydrogenase — NADH complex [PDF]

FEBS Letters, 1977
Transient kinetic studies of the mechanism of lactate dehydrogenase were helped considerably by the variety of optical signals available. This permitted the direct observation of the formation and interconversion of intermediates [l] . The formation of the binary complex, E. NADH, from free enzyme and reduced nucleotide can be observed both through the
Holbrook, J.J., Hardman, M.J., Parker, D.M., Yates, D.W., Gutfreund, H.   +4 more
openaire   +2 more sources

Hydrostatic pressure activates HIF‐1α via β‐catenin to promote stemness in breast cancer cells

FEBS Open Bio, EarlyView.
To mimic the elevated intestinal fluid pressure in breast cancers, we loaded human breast cancer cells (MCF‐7, MDA‐MB‐453, and BT‐474) to 50 mmHg hydrostatic pressure. Hydrostatic pressure exposure upregulated HIF‐1α and induced stemness in MCF‐7 and BT‐474 cells.
Da Zhai, Yong Xu, Jingjing Ma, Ratchanee Duangrat, Chen Yan, Jingyan Liang, Hua Wei, Bin Jiang, Tao‐Sheng Li   +8 more
wiley   +1 more source

Blood AST, ALT and UREA/BUN Level Analysis

Bio-Protocol, 2013
AST (aspartate aminotransferase; GOT, glutamate oxalacetate transaminase) and ALT (alanine aminotransferase; GPT, glutamate pyruvate transaminase) are sensitive indicators to monitor the liver function under drugs treatment or with acute viral hepatitis.
Yuh-Pyng Sher, Mien-Chie Hung
doaj   +1 more source

Relocation of chloroplast proteins from cytosols into chloroplasts

Plant Signaling & Behavior, 2023
The chloroplasts in terrestrial plants play a functional role as a major sensor for perceiving physiological changes under normal and stressful conditions.
Kwanuk Lee
doaj   +1 more source

The Yeast Complex I Equivalent NADH Dehydrogenase Rescues pink1 Mutants

PLoS Genetics, 2012
Pink1 is a mitochondrial kinase involved in Parkinson's disease, and loss of Pink1 function affects mitochondrial morphology via a pathway involving Parkin and components of the mitochondrial remodeling machinery.
Sven Vilain, G. Esposito, D. Haddad, O. Schaap, Mariya P. Dobreva, Melissa Vos, S. Van Meensel, V. Morais, B. de Strooper, P. Verstreken   +9 more
semanticscholar   +1 more source

PRMT1‐Mediated LDHA Methylation Drives STAT3 Lactylation to Orchestrate Intestinal Inflammation and Tumorigenesis

Advanced Science, EarlyView.
This study identifies an immunometabolic axis wherein SAM‐driven PRMT1 methylates LDHA, enhancing its activity. The resultant lactate induces STAT3 K709 lactylation, which stabilizes an active conformation to promote STAT3 phosphorylation and IL‐10 expression.
Hui Wang, Mengyu Zhang, Weipeng Gong, Jiaxuan Wu, Junping Zhang, Wenxiu Zhang, Yue Liu, Kui Wang, Canhua Huang, Jun Zhou, Sijin Wu, Yan Li, Tianliang Li   +12 more
wiley   +1 more source

Decompartmentalization of the yeast mitochondrial metabolism to improve chemical production in Issatchenkia orientalis

Nature Communications
Microbial production of chemicals may suffer from inadequate cofactor provision, a challenge further exacerbated in yeasts due to compartmentalized cofactor metabolism.
Vinh G. Tran, Shih-I Tan, Hao Xu, Daniel R. Weilandt, Xi Li, Sarang S. Bhagwat, Zhixin Zhu, Jeremy S. Guest, Joshua D. Rabinowitz, Huimin Zhao   +9 more
doaj   +1 more source

COFACTOR SPECIFICITY ENGINEERING OF STREPTOCOCCUS MUTANS NADH OXIDASE 2 FOR NAD(P)+ REGENERATION IN BIOCATALYTIC OXIDATIONS

Computational and Structural Biotechnology Journal, 2014
Soluble water-forming NAD(P)H oxidases constitute a promising NAD(P)+ regeneration method as they only need oxygen as cosubstrate and produce water as sole byproduct. Moreover, the thermodynamic equilibrium of O2 reduction is a valuable driving force for
Barbara Petschacher, Nicole Staunig, Monika Müller, Martin Schürmann, Daniel Mink, Stefaan De Wildeman, Karl Gruber, Anton Glieder   +7 more
doaj   +1 more source

Disruption of Treg Homeostasis in Rheumatoid Arthritis via Ferroptosis‐Mediated ETC Collapse and TXK‐STAT3/PLCγ1 Activation

Advanced Science, EarlyView.
In rheumatoid arthritis, synovial Tregs accumulate but are functionally impaired due to iron overload‐induced ferroptosis. This triggers mitochondrial dysfunction and TXK tyrosine kinase‐mediated signaling, leading to Treg destabilization and inflammation.
Jingrong Chen, Xiao Guan, Kexu Xiong, Xiaoyi Shi, Luyao Wu, Chuanxin Su, Jun Zhao, Rongzhen Liang, Huimin Wang, Junlong Dang, Ye Chen, Yiding Xiong, Chi Zhou, Futao Zhao, Zhenhang Zhu, Xiaoli Fan, Li Zhou, Nancy Olsen, Yutong Jiang, Song Guo Zheng   +19 more
wiley   +1 more source

Variation and evolution of polyadenylation profiles in sauropsid mitochondrial mRNAs as deduced from the high-throughput RNA sequencing

BMC Genomics, 2017
Background Genes encoded in vertebrate mitochondrial DNAs are transcribed as a polycistronic transcript for both strands, which is later processed into individual mRNAs, rRNAs and tRNAs, followed by modifications, such as polyadenylation at the 3′ end of
Yao Sun, Masaki Kurisaki, Yasuyuki Hashiguchi, Yoshinori Kumazawa   +3 more
doaj   +1 more source