Results 1 to 10 of about 81,638 (208)

NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems

Nature Communications, 2023
NADP(H) is a central metabolic hub providing reducing equivalents to multiple biosynthetic, regulatory and antioxidative pathways in all living organisms.
Pamela E Molinari, A. Krapp, Andrea M J Weiner, Hannes M. Beyer, Arun Kumar Kondadi, Tim Blomeier, Melina López, Pilar Bustos-Sanmamed, Evelyn Tevere, W. Weber, A. Reichert, N. Calcaterra, Mathias Beller, N. Carrillo, Matias D. Zurbriggen   +14 more
semanticscholar   +1 more source

Mitochondrial NADP(H) generation is essential for proline biosynthesis

Science, 2021
Providing for protein synthesis Compartmentalization of metabolic processes into organelles can have important consequences. Zhu et al. examined the role of the coenzyme nicotinamide adenine dinucleotide phosphate (NADP+) and its reduced form (NADPH) in ...
Jiajun Zhu, Simon Schwörer, Mirela Berisa, Yeon Ju Kyung, K. W. Ryu, Junmei Yi, Xuejun Jiang, J. Cross, C. Thompson   +8 more
semanticscholar   +1 more source

G6PD-mediated increase in de novo NADP+ biosynthesis promotes antioxidant defense and tumor metastasis

Science Advances, 2022
Metastasizing cancer cells are able to withstand high levels of oxidative stress through mechanisms that are poorly understood. Here, we show that under various oxidative stress conditions, pancreatic cancer cells markedly expand NADPH and NADP+ pools ...
Yang Zhang, Yi Xu, Wenyun Lu, Jinyang Li, Sixiang Yu, E. Brown, B. Stanger, J. Rabinowitz, Xiaolu Yang   +8 more
semanticscholar   +1 more source

Mitochondrial NADP+ is essential for proline biosynthesis during cell growth

Nature Metabolism, 2021
Nicotinamide adenine dinucleotide phosphate (NADP+) is vital to produce NADPH, a principal supplier of reducing power for biosynthesis of macromolecules and protection against oxidative stress.
D. H. Tran, R. Kesavan, Halie Rion, Mona H Soflaee, Ashley Solmonson, Divya Bezwada, Hieu S. Vu, Feng Cai, J. Phillips, R. Deberardinis, G. Hoxhaj   +10 more
semanticscholar   +1 more source

Nanomechanical Study of Enzyme: Coenzyme Complexes: Bipartite Sites in Plastidic Ferredoxin-NADP+ Reductase for the Interaction with NADP+

Antioxidants, 2022
Plastidic ferredoxin-NADP+ reductase (FNR) transfers two electrons from two ferredoxin or flavodoxin molecules to NADP+, generating NADPH. The forces holding the Anabaena FNR:NADP+ complex were analyzed by dynamic force spectroscopy, using WT FNR and ...
Sandra Pérez-Domínguez, Silvia Caballero-Mancebo, C. Marcuello, M. Martínez-Júlvez, M. Medina, A. Lostao   +5 more
semanticscholar   +1 more source

Allosteric role of a structural NADP+ molecule in glucose-6-phosphate dehydrogenase activity

Proceedings of the National Academy of Sciences of the United States of America, 2022
Significance Glucose-6-phosphate dehydrogenase (G6PD) is the main cellular source of NADPH and plays a key role in maintaining reduced glutathione to protect cells from oxidative stress disorders such as hemolytic anemia.
Xuepeng Wei, Kathryn Kixmoeller, Elana E Baltrusaitis, Xiaolu Yang, R. Marmorstein   +4 more
semanticscholar   +1 more source

Auxin is involved in arbuscular mycorrhizal fungi-promoted tomato growth and NADP-malic enzymes expression in continuous cropping substrates

BMC Plant Biology, 2021
Background Despite significant limitations of growth medium reuse, a large amount of organic substrate is reused in soilless cultivation of horticultural crops in China. Arbuscular mycorrhizal fungi (AMF) can promote nutrient absorption and improve plant
Yu Wang, Wenze Zhang, Weikang Liu, G. Ahammed, Wenxu Wen, Shi-rong Guo, S. Shu, Jin Sun   +7 more
semanticscholar   +1 more source

In Vivo Selection for Formate Dehydrogenases with High Efficiency and Specificity toward NADP+

ACS Catalysis, 2020
The efficient regeneration of cofactors is vital for the establishment of biocatalytic processes. Formate is an ideal electron donor for cofactor regeneration due to its general availability, low reduction potential, and benign byproduct (CO2).
Liliana Calzadiaz-Ramirez, Carla Calvó-Tusell, Gabriele M. M. Stoffel, Steffen N. Lindner, Sílvia Osuna, T. Erb, M. Garcia‐Borràs, A. Bar‐Even, C. Acevedo‐Rocha   +8 more
semanticscholar   +1 more source

Plasmodium falciparum glutamate dehydrogenase a is dispensable and not a drug target during erythrocytic development [PDF]

, 2011
<p>Background: Plasmodium falciparum contains three genes encoding potential glutamate dehydrogenases. The protein encoded by gdha has previously been biochemically and structurally characterized.
Aparicio, I., Engel, P.C., Llinas, M., Muller, S., Olszewski, K., Patzewitz, E.-M., Perner, J., Storm, J.   +7 more
core   +4 more sources

Cofactor regeneration by a soluble pyridine nucleotide transhydrogenase for biological production of hydromorphone [PDF]

, 2000
We have applied the soluble pyridine nucleotide transhydrogenase of Pseudomonas fluorescens to a cell-free system for the regeneration of the nicotinamide cofactors NAD and NADP in the biological production of the important semisynthetic opiate drug ...
Boonstra, B, Bruce, N C, French, C E, Rathbone, D A, Walker, E H   +4 more
core   +2 more sources