NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems [PDF]
Nature Communications, 2023 NADP(H) is a central metabolic hub providing reducing equivalents to multiple biosynthetic, regulatory and antioxidative pathways in all living organisms.
Pamela E. Molinari +14 more
doaj +3 more sources
The regulation and catalytic mechanism of the NADP-malic enzyme from tobacco leaves [PDF]
Journal of the Serbian Chemical Society, 2009 The non-photosynthetic NADP-malic enzyme EC 1.1.1.40 (NADP-ME), which catalyzes the oxidative decarboxylation of L-malate and NADP+ to produce pyruvate and NADPH, respectively, and which could be involved in plant defense responses, was isolated from ...
VERONIKA DOUBNEROVÁ +3 more
doaj +5 more sources
NADP(H) allosterically regulates the interaction between ferredoxin and ferredoxin‐NADP+ reductase [PDF]
FEBS Open Bio, 2019 Ferredoxin‐NADP+ reductase (FNR) in plants receives electrons from ferredoxin (Fd) at the end of the photosynthetic electron transfer chain and converts NADP+ to NADPH. The interaction between Fd and FNR in plants was previously shown to be attenuated by
Yoko Kimata‐Ariga +6 more
doaj +4 more sources
NADP-Dependent Malic Enzyme 1 Participates in the Abscisic Acid Response in Arabidopsis thaliana [PDF]
Frontiers in Plant Science, 2018 Arabidopsis thaliana possesses three cytosolic (NADP-ME1-3) and one plastidic (NADP-ME4) NADP-dependent malic enzymes. NADP-ME2 and -ME4 show constitutive expression, in contrast to NADP-ME1 and -ME3, which are restricted to particular tissues.
Cintia L. Arias +9 more
doaj +5 more sources
Adjustment of light-responsive NADP dynamics in chloroplasts by stromal pH
Nature Communications, 2023 Cyclic electron transfer (CET) predominates when NADP+ is at basal levels, early in photosynthetic induction; however, the mechanism underlying the subsequent supply of NADP+ to fully drive steady-state linear electron transfer remains unclear.
Yusuke Fukuda +3 more
doaj +1 more source
NADP(H)-dependent biocatalysis without adding NADP(H)
Proceedings of the National Academy of Sciences, 2022 Isocitrate dehydrogenase 1 (IDH1) naturally copurifies and crystallizes in a resting state with a molecule of its exchangeable cofactor, NADP + /NADPH, bound in each monomer of the homodimer. We report electrochemical studies with IDH1 that exploit this property to reveal the massive advantage of nanoconfinement to ...
Herold, RA +3 more
openaire +2 more sources
Bio-Protocol, 2023 Chloroplast NADP-dependent malate dehydrogenase (NADP-MDH) is a redox regulated enzyme playing an important role in plant redox homeostasis. Leaf NADP-MDH activation level is considered a proxy for the chloroplast redox status.
Kevin Baudry +1 more
doaj +1 more source
Journal of Plant Interactions, 2022 High temperature inhibits the growth and development of maize (Zea mays L.) and wheat (Triticum aestivum L.) seedlings. The current study attempts to elucidate the underlying mechanisms of exogenous trehalose for the improvement of maize and wheat ...
Ziwei Zhang, Mei Sun, Yamin Gao, Yin Luo
doaj +1 more source
Preferential utilization of NADPH as the endogenous electron donor for NAD(P)H:quinone oxidoreductase 1 (NQO1) in intact pulmonary arterial endothelial cells [PDF]
, 2009 The goal was to determine whether endogenous cytosolic NAD(P)H:quinone oxidoreductase 1 (NQO1) preferentially uses NADPH or NADH in intact pulmonary arterial endothelial cells in culture.
Bongard, Robert D +3 more
core +2 more sources
Measurement of Chloroplastic NAD Kinase Activity and Whole Tissue NAD Kinase Assay
Bio-Protocol, 2020 Nicotinamide adenine dinucleotide phosphate (NADP) synthesis requires nicotinamide adenine dinucleotide (NAD) kinase activity, substrate NAD and ATP.
Yuma Ishikawa +2 more
doaj +1 more source