Results 71 to 80 of about 10,930 (224)

Limited proteolysis of maize NADP-malic enzyme.

Indian journal of biochemistry & biophysics, 2012
The incubation of maize malic enzyme at 37 degrees C with trypsin at a ratio of 150:1 of malic enzyme to trypsin caused rapid and complete inactivation of enzyme activity. The inactivation was caused by fairly specific cleavage of the enzyme monomer (62 kDa) into 40 kDa and 20 kDa fragments.
S, Pinto, S R, Rao, A S, Bhagwat
openaire   +1 more source

Light-stimulated synthesis of NADP malic enzyme in leaves of maize.

Journal of Biological Chemistry, 1983
Illumination of etiolated maize plants for 80 h brings about a 15-20-fold increase in activity of NADP malic enzyme (EC 1.1.1.40). Increases in NADP malic enzyme protein and in the level of translatable mRNA for this protein occur simultaneously with the activity increase.
P D, Collins, D R, Hague
openaire   +2 more sources

A new branch of mammalian vitamin B6 metabolism: AKR1C‐mediated conversion of pyridoxal to pyridoxine and 4‐pyridoxolactone

The FEBS Journal, EarlyView.
Pyridoxal 5′‐phosphate (PLP) homeostasis relies on salvage enzymes, yet key metabolic branches remain undefined. We identify AKR1C isozymes as previously undescribed contributors that convert pyridoxal into pyridoxine or 4‐pyridoxolactone through reductase and dehydrogenase activities.
Nayu Kito, Yasuyuki Kitaura, Kyoka Iino, Kazuya Toriumi, Hyunah Noh, Naoya Ogawa, Makoto Arai, Hisashi Hemmi, Tomokazu Ito   +8 more
wiley   +1 more source

Malic Acid Production by Saccharomyces cerevisiae: Engineering of Pyruvate Carboxylation, Oxaloacetate Reduction, and Malate Export

, 2008
Malic acid is a potential biomass-derivable "building block" for chemical synthesis. Since wild-type Saccharomyces cerevisiae strains produce only low levels of malate, metabolic engineering is required to achieve efficient malate production with this ...
Van Maris, A.J.A. (author), Dijkema, C., Van Winden, W.A. (author), Winden, W.A., van, Dijkema, C. (author), Zelle, R.M. (author), Van Maris, A.J.A., Van Winden, W.A., De Hulster, E., Geertman, J.M., Pronk, J.T. (author), Hulster, E., de, Geertman, J.M.A., Winkler, A.A. (author), Van Dijken, J.P., Pronk, J.T., Winkler, A.A., De Hulster, E. (author), De Waard, P. (author), Geertman, J.M. (author), Waard, P., de, Zelle, R.M., De Waard, P., Van Dijken, J.P. (author)   +23 more
core   +1 more source

Magnesium deficiency-induced changes in organic acid metabolism of Citrus sinensis roots and leaves

Biologia Plantarum, 2013
Organic acid (OA) metabolisms are of fundamental importance but very limited data are available on the responses of plant OA metabolisms to Mg-deficiency. Seedlings of Citrus sinensis (L.) Osbeck cv. Xuegan were irrigated with Mg-deficient (0, 50, or 500
L.T. Yang, G.H. Yang, X. You, C.P. Zhou, Y.B. Lu, L.S. Chen   +5 more
doaj   +1 more source

Steroidogenic compensation and lipid deficiency with enhanced NAD+ salvage in small‐for‐gestational‐age placenta

The FEBS Journal, EarlyView.
Fetal growth restriction is associated with placental metabolic adaptations. In small‐for‐gestational‐age placenta (SGA), cholesterol receptors and steroidogenic enzymes are upregulated, enhancing steroidogenesis. NAD salvage pathway is also increased to support NADP+/NADPH requirements.
Serena Xodo, Lorenza Driul, Vanessa Tolotto, Eros Di Giorgio, Luigi E. Xodo   +4 more
wiley   +1 more source

Fumarate analogs act as allosteric inhibitors of the human mitochondrial NAD(P)+-dependent malic enzyme. [PDF]

PLoS ONE, 2014
Human mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME) is allosterically activated by the four-carbon trans dicarboxylic acid, fumarate. Previous studies have suggested that the dicarboxylic acid in a trans conformation around the carbon-carbon
Ju-Yi Hsieh, Jyung-Hurng Liu, Pai-Chun Yang, Chi-Li Lin, Guang-Yaw Liu, Hui-Chih Hung   +5 more
doaj   +1 more source

1H NMR metabolomics and lipidomics analysis of neutrophils reveals biomarkers of ageing, inflammageing and frailty

The FEBS Journal, EarlyView.
Using 1H NMR metabolomics, we measured polar and lipid metabolites from human blood neutrophils from people with frailty (n = 31, mean age 84Y), people with rheumatoid arthritis (n = 16, mean age 55Y), robust older (n = 24, mean age 66Y) and healthy younger people (n = 21, mean age 22Y).
Genna Ali Abdullah, Grace Filbertine, Lucy Gill, Asangaedem Akpan, Marie M. Phelan, Helen Louise Wright   +5 more
wiley   +1 more source

Malic enzyme : Its purification and characterization from Mucor circinelloides and occurrence in other oleaginous fungi [PDF]

, 1997
Malic enzyme was purified 43-fold from Mucor circinelloides. The enzyme was dependent on Mg2+ or Mn2+ for activity, was not active with Dmalate and had a pH optimum at 7.8.
Ratledge, C., Wynn, J., Savitha, J.P.
core   +1 more source

NADP-Dependent Malic Enzyme 1 Participates in the Abscisic Acid Response in Arabidopsis thaliana

Frontiers in Plant Science, 2018
Arabidopsis thaliana possesses three cytosolic (NADP-ME1-3) and one plastidic (NADP-ME4) NADP-dependent malic enzymes. NADP-ME2 and -ME4 show constitutive expression, in contrast to NADP-ME1 and -ME3, which are restricted to particular tissues.
Cintia L. Arias, Tatiana Pavlovic, Giuliana Torcolese, Mariana B. Badia, Mauro Gismondi, Verónica G. Maurino, Carlos S. Andreo, María F. Drincovich, Mariel C. Gerrard Wheeler, Mariana Saigo   +9 more
doaj   +1 more source