In Vivo Selection for Formate Dehydrogenases with High Efficiency and Specificity toward NADP+
ACS Catalysis, 2020 The efficient regeneration of cofactors is vital for the establishment of biocatalytic processes. Formate is an ideal electron donor for cofactor regeneration due to its general availability, low reduction potential, and benign byproduct (CO2).
Liliana Calzadiaz-Ramirez +8 more
semanticscholar +1 more source
Determinants of nucleotide-binding selectivity of malic enzyme. [PDF]
PLoS ONE, 2011 Malic enzymes have high cofactor selectivity. An isoform-specific distribution of residues 314, 346, 347 and 362 implies that they may play key roles in determining the cofactor specificity. Currently, Glu314, Ser346, Lys347 and Lys362 in human c-NADP-ME
Ju-Yi Hsieh +2 more
doaj +1 more source
Cofactor regeneration by a soluble pyridine nucleotide transhydrogenase for biological production of hydromorphone [PDF]
, 2000 We have applied the soluble pyridine nucleotide transhydrogenase of Pseudomonas fluorescens to a cell-free system for the regeneration of the nicotinamide cofactors NAD and NADP in the biological production of the important semisynthetic opiate drug ...
Boonstra, B +4 more
core +2 more sources
Measurement of Chloroplastic NAD Kinase Activity and Whole Tissue NAD Kinase Assay
Bio-Protocol, 2020 Nicotinamide adenine dinucleotide phosphate (NADP) synthesis requires nicotinamide adenine dinucleotide (NAD) kinase activity, substrate NAD and ATP.
Yuma Ishikawa +2 more
doaj +1 more source
Preferential utilization of NADPH as the endogenous electron donor for NAD(P)H:quinone oxidoreductase 1 (NQO1) in intact pulmonary arterial endothelial cells [PDF]
, 2009 The goal was to determine whether endogenous cytosolic NAD(P)H:quinone oxidoreductase 1 (NQO1) preferentially uses NADPH or NADH in intact pulmonary arterial endothelial cells in culture.
Bongard, Robert D +3 more
core +2 more sources
NADP(+)-isocitrate dehydrogenase from the cyanobacterium Anabaena sp. strain PCC 7120: purification and characterization of the enzyme and cloning, sequencing, and disruption of the icd gene [PDF]
, 1994 NADP(+)-isocitrate dehydrogenase (NADP(+)-IDH) from the dinitrogen-fixing filamentous cyanobacterium Anabaena sp. strain PCC 7120 was purified to homogeneity.
Florencio Bellido, Francisco Javier +1 more
core +2 more sources
Identification and evolution of C4 photosynthetic pathway genes in plants
BMC Plant Biology, 2020 Background NADP-malic enzyme (NAPD-ME), and pyruvate orthophosphate dikinase (PPDK) are important enzymes that participate in C4 photosynthesis. However, the evolutionary history and forces driving evolution of these genes in C4 plants are not completely
Weiping Shi +7 more
doaj +1 more source
Adaptation responses in C4 photosynthesis of sweet maize (Zea mays L.) exposed to nicosulfuron
Ecotoxicology and Environmental Safety, 2021 Nicosulfuron is an ingredient in photosynthesis-inhibiting herbicides and has been widely used in corn post-emergence weed control. In the current study, a pair of sister lines, HK301 (nicosulfuron-tolerence, NT) and HK320 (nicosulfuron-sensitive, NS ...
Jian Wang +6 more
doaj +1 more source
A spatial analysis of physiological changes associated with infection of cotyledons of marrow plants with cucumber mosaic virus [PDF]
, 1996 Changes in host primary metabolism associated with the compatible interaction between cucumber mosaic virus and cotyledons of the marrow plant (Cucurbita pepo L.) have been localized, first by measuring activities of key enzymes in infected and ...
Leegood, R.C. +3 more
core +3 more sources
NADP+ reduction by human lymphocytes [PDF]
Clinical and Experimental Immunology, 1990 SUMMARY The hexose monophosphate shunt (HMPS) is known to be responsible for the reduction of NADP+ by lymphocytes. We tried to find other enzymatic systems that might provide the lymphocytes with NADPH. By measuring the absorbance at 340 nm we noted that the addition of NADP+ to a preparation of disrupted lymphocytes resulted in the ...
A, Klein +3 more
openaire +2 more sources