Results 1 to 10 of about 733 (118)

NADH dehydrogenase of Corynebacterium glutamicum. Purification of an NADH dehydrogenase II homolog able to oxidize NADPH [PDF]

FEMS Microbiology Letters, 2001
NADPH oxidase activity, in addition to NADH oxidase activity, has been shown to be present in the respiratory chain of Corynebacterium glutamicum. In this study, we tried to purify NADPH oxidase and NADH dehydrogenase activities from the membranes of C. glutamicum.
Kazunobu Matsushita, M Iwahashi, O Adachi   +2 more
exaly   +3 more sources

Theoretical Analysis of the Built-in Metabolic Pathway Effect on the Metabolism of Erythrocyte-Bioreactors That Neutralize Ammonium

Metabolites, 2021
The limitations of the efficiency of ammonium-neutralizing erythrocyte-bioreactors based on glutamate dehydrogenase and alanine aminotransferase reactions were analyzed using a mathematical model.
Evgeniy Protasov, Larisa Koleva, Elizaveta Bovt, Fazoil I. Ataullakhanov, Elena Sinauridze   +4 more
doaj   +1 more source

NAD- and NADPH-Contributing Enzymes as Therapeutic Targets in Cancer: An Overview

Biomolecules, 2020
Actively proliferating cancer cells require sufficient amount of NADH and NADPH for biogenesis and to protect cells from the detrimental effect of reactive oxygen species.
Alvinsyah Adhityo Pramono, Gulam M. Rather, Herry Herman, Keri Lestari, Joseph R. Bertino   +4 more
doaj   +1 more source

Divergent effects of glutathione depletion on isocitrate dehydrogenase 1 and the pentose phosphate pathway in hamster liver

Physiological Reports, 2020
The liver regenerates NADPH via multiple pathways to maintain redox balance and reductive biosynthesis. The pentose phosphate pathway (PPP) contributes to hepatic lipogenesis by supplying NADPH, and it is thought to play a major role in response to ...
Eunsook S. Jin, Min H. Lee, Craig R. Malloy   +2 more
doaj   +1 more source

The central role of NADPH depletion in MnTE-2-PyP-induced prostate cancer cell growth inhibition

Advances in Redox Research, 2021
Radiotherapy is commonly used in prostate cancer treatment. MnTE-2-PyP is a superoxide dismutase (SOD) mimic that can protect normal tissues from radiation toxicities while simultaneously promoting radiation-induced damage to cancer tissues.
Yuxiang Zhu, Divya Murthy, Sai Sundeep Kollala, Elizabeth A. Kosmacek, Arpita Chatterjee, Joshua A. McDowell, Pankaj K. Singh, Rebecca E. Oberley-Deegan   +7 more
doaj   +1 more source

Efficient regeneration of NADPH using an engineered phosphite dehydrogenase [PDF]

Biotechnology and Bioengineering, 2006
AbstractThe in situ regeneration of reduced nicotinamide cofactors (NAD(P)H) is necessary for practical synthesis of many important chemicals. Here, we report the engineering of a highly stable and active mutant phosphite dehydrogenase (12x‐A176R PTDH) from Pseudomonas stutzeri and evaluation of its potential as an effective NADPH regeneration system ...
Tyler W, Johannes, Ryan D, Woodyer, Huimin, Zhao   +2 more
openaire   +2 more sources

A Hidden Transhydrogen Activity of a FMN-Bound Diaphorase under Anaerobic Conditions. [PDF]

PLoS ONE, 2016
BACKGROUND:Redox cofactors of NADH/NADPH participate in many cellular metabolic pathways for facilitating the electron transfer from one molecule to another in redox reactions. Transhydrogenase plays an important role in linking catabolism and anabolism,
John Collins, Ting Zhang, Scott Huston, Fangfang Sun, Y-H Percival Zhang, Jinglin Fu   +5 more
doaj   +1 more source

Knocking out central metabolism genes to identify new targets and alternating substrates to improve lipid synthesis in Y. lipolytica

Frontiers in Bioengineering and Biotechnology, 2023
Introduction: Systematic gene knockout studies may offer us novel insights on cell metabolism and physiology. Specifically, the lipid accumulation mechanism at the molecular or cellular level is yet to be determined in the oleaginous yeast Y.
Jiang Zhu, Yang Gu, Yang Gu, Yijing Yan, Jingbo Ma, Jingbo Ma, Xiaoman Sun, Peng Xu, Peng Xu, Peng Xu   +9 more
doaj   +1 more source

Change in Cofactor Specificity of Oxidoreductases by Adaptive Evolution of an Escherichia coli NADPH-Auxotrophic Strain

mBio, 2021
The nicotinamide cofactor specificity of enzymes plays a key role in regulating metabolic processes and attaining cellular homeostasis. Multiple studies have used enzyme engineering tools or a directed evolution approach to switch the cofactor preference
Madeleine Bouzon, Volker Döring, Ivan Dubois, Anne Berger, Gabriele M. M. Stoffel, Liliana Calzadiaz Ramirez, Sophia N. Meyer, Marion Fouré, David Roche, Alain Perret, Tobias J. Erb, Arren Bar-Even, Steffen N. Lindner   +12 more
doaj   +1 more source

A dehydrogenase-mediated recycling system of NADPH in plant peroxisomes [PDF]

Biochemical Journal, 1998
The presence of the two NADP-dependent dehydrogenases of the pentose phosphate pathway has been investigated in plant peroxisomes from pea (Pisum sativum L.) leaves. Both enzymes, glucose-6-phosphate dehydrogenase (G6PDH; EC 1.1.1.49) and 6-phosphogluconate dehydrogenase (6PGDH; EC 1.1.1.44), were present in the matrix of leaf peroxisomes, and their ...
F J, Corpas, J B, Barroso, L M, Sandalio, S, Distefano, J M, Palma, J A, Lupiáñez, L A, Del Río   +6 more
openaire   +2 more sources