Results 121 to 130 of about 16,460 (261)

Ion Activation Methods for Top‐Down Proteomics

Mass Spectrometry Reviews, EarlyView.
ABSTRACT Mass spectrometry (MS) has emerged as a premier method used to characterize the sequences of proteins. Top‐down proteomics aims to capture the multiple sources of structural diversity reflected in proteins, such as those that arise from alternative RNA splicing events or the addition of post‐translational modifications. Tandem MS (i.e., MS/MS)
Jada N. Walker, Jennifer S. Brodbelt
wiley   +1 more source

Integrative Modeling in the Age of Machine Learning: A Summary of HADDOCK Strategies in CAPRI Rounds 47–55

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT The HADDOCK team participated in CAPRI rounds 47–55 as server, manual predictor, and scorers. Throughout these CAPRI rounds, we used a plethora of computational strategies to predict the structure of protein complexes. Of the 10 targets comprising 24 interfaces, we achieved acceptable or better models for 3 targets in the human category and 1 ...
Victor Reys, Marco Giulini, Vlad Cojocaru, Anna Engel, Xiaotong Xu, Jorge Roel‐Touris, Cunliang Geng, Francesco Ambrosetti, Brian Jiménez‐García, Zuzana Jandova, Panagiotis I. Koukos, Charlotte van Noort, João M. C. Teixeira, Siri C. van Keulen, Manon Réau, Rodrigo V. Honorato, Alexandre M. J. J. Bonvin   +16 more
wiley   +1 more source

Selection of Functional Intracellular Nanobodies

SLAS Discovery, 2019
Camelid-derived nanobodies are versatile tools for research, diagnostics, and therapeutics. Certain nanobodies can function as intrabodies and bind antigens within the eukaryotic cytosol. This capability is valuable for the development of intracellular probes and targeted gene therapies.
openaire   +2 more sources

A method for rapid nanobody screening with no bias of the library diversity

iScience
Summary: Nanobody, referred to the variable domain of heavy-chain-only antibodies, has several advantages such as small size and feasible Escherichia coli expression, making them promising for scientific research and therapies.
Zhiqing Tao, Xiaoling Zhao, Huan Wang, Juan Zhang, Guosheng Jiang, Bin Yu, Yihao Chen, Mingjun Zhu, Junli Long, Lei Yin, Xu Zhang, Maili Liu, Lichun He   +12 more
doaj   +1 more source

AlphaFold and Docking Approaches for Antibody–Antigen and Other Targets: Insights From CAPRI Rounds 47–55

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT Accurate modeling of the structures of protein–protein complexes and other biomolecular interactions represents a longstanding and important challenge for computational biology. The Critical Assessment of PRedicted Interactions (CAPRI) experiment has served for over two decades as a key means to assess and compare current approaches and ...
Ragul Gowthaman, Minjae Park, Rui Yin, Johnathan D. Guest, Brian G. Pierce   +4 more
wiley   +1 more source

NanoBERTa-ASP: predicting nanobody paratope based on a pretrained RoBERTa model

BMC Bioinformatics
Background Nanobodies, also known as VHH or single-domain antibodies, are unique antibody fragments derived solely from heavy chains. They offer advantages of small molecules and conventional antibodies, making them promising therapeutics.
Shangru Li, Xiangpeng Meng, Rui Li, Bingding Huang, Xin Wang   +4 more
doaj   +1 more source

Targeting mGlyR with nanobodies for depression

Nature Communications
Abstract Development of therapies for neuropsychiatric conditions is one of the greatest challenges of modern medicine. Common limitations of traditional small molecule drugs include poor efficacy, off-target side effects and difficult druggability of many targets.
Laboute, Thibaut, Zucca, Stefano, Sial, Omar, Sharma, Mansi, Brunori, Gloria, Singh, Shikha, Nageswar, Kv, Peng, Haiyong, Rader, Christoph, Becker, Jérôme Aj, Le Merrer, Julie, Singh, Appu, Martemyanov, Kirill   +12 more
openaire   +3 more sources

Nanobody-thioesterase chimeras to specifically target protein palmitoylation

 The complexity of the cellular proteome is massively expanded by a repertoire of chemically distinct reversible post-translational modifications (PTMs) that control protein localisation, interactions, and function.
William Fuller (3875263), Emma Dunning (20691914), William Mullen (103621), Caglar Gok (18964531), Fiona Jordan (5747414), Emily K. Gallen (20691911), Sheon Mary (3696952), Samuel Adu (20691908), Alan D. Robertson (19336523), Chien-Wen Kuo (13908834), Hannah Fulton (20697910), Godfrey L. Smith (8152389), Eleanor Dickson-Murray (20691905)   +12 more
core  

A novel nanobody against urease activity of Helicobacter pylori

, 2013
SummaryBackgroundHelicobacter pylori infection is associated with gastritis and in some cases with gastric and duodenal ulcers, and even adenocarcinoma. Antibiotic therapy has significant limitations, such as the high cost and the emergence of antibiotic-
Bakherad, Hamid, Ardekani, Leila Safaee, Rasooli, Iraj, Bazl, Masoumeh Rajabi, Ebrahimizadeh, Walead, Zare, Hamed, Gargari, Seyed Latif Mousavi, Mohammadi, Mohammad   +7 more
core   +1 more source

Massive Sampling Strategy for Antibody–Antigen Targets in CAPRI Round 55 With MassiveFold

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT Massive sampling with AlphaFold2 improves protein–protein complex predictions. This has been shown during the last CASP15‐CAPRI blind prediction round by the AFsample tool. However, more difficult targets including antibody–antigen binding remain challenging. CAPRI Round 55 consisted of three antibody–antigen targets and one heterotrimer.
Nessim Raouraoua, Marc F. Lensink, Guillaume Brysbaert   +2 more
wiley   +1 more source