Results 131 to 140 of about 16,460 (261)

In Vitro Self-assembly Study of ZEN/DON Anti-idiotype Nanobody Based on SpyCatcher/SpyTag

Shipin gongye ke-ji
To improve the immunoassay performance of mycotoxin anti-idiotype nanobody and to realize mimicry of bispecific detected antigens, in this study, anti-idiotype nanobody against zearalenone (ZEN) and deoxynivalenol (DON) were used, and the SpyCatcher ...
Xiao WU, Yun LIANG, Yuhong ZHONG, Yuanzhen GUO, Binhui HUANG, Qinghua HE   +5 more
doaj   +1 more source

Analysing hot spots in nanobody-antigen complexes

, 2023
Nanotelesa so proteini izvedeni iz posebne vrste imunoglobulinov, ki imajo samo težko verigo in imajo zaradi tega posebne lastnosti. Dodaten vpogled v način prepoznave antigenov z nanotelesi lahko dobimo z analizo energijsko močnejših interakcij (ang ...
Zgonik, Timotej
core  

Biomolecular Interaction Prediction in the Pre‐ and Post‐AlphaFold Era: The 8th CAPRI Evaluation

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT We report on the 8th CAPRI Evaluation period, capturing the assessment of CAPRI Rounds 47 to 55 (excluding the CASP and COVID‐related Rounds), which have witnessed the transition to AI‐driven prediction tools such as AlphaFold and related alternatives. The prediction Rounds in this evaluation are characterized by a high level of difficulty due
Marc F. Lensink, Nessim Raouraoua, Guillaume Brysbaert, Sameer Velankar, Shoshana J. Wodak, Alexandre M. J. J. Bonvin   +5 more
wiley   +1 more source

Improving the production and stability of nanobodies

Protein Engineering, Design and Selection
Abstract Nanobodies offer unique advantages in biomedical and biotechnological applications due to their smaller size, ability to bind challenging epitopes, and affordable production using recombinant technology. However, challenges in large-scale production, stability, and solubility limit their widespread use.
Alba Pejenaute, Laura Gálvez-Larrosa, Cristina M Segovia, Pablo Herrero-Alfonso, David Fernández-Ramos, Fernando Lopitz-Otsoa, Óscar Millet, Francesca Peccati, Gonzalo Jiménez-Osés, Gabriel Ortega-Quintanilla   +9 more
openaire   +2 more sources

The efficacy of current vaccines and novel Nanobody-based antivirals against highly pathogenic rabies and lyssaviruses

, 2010
Current rabies virus (RABV; lyssavirus genotype 1) vaccines and RABV immunoglobulins (RIG) confer a robust protective immunity against genotype 1 isolates.
van Gucht, S., Vaughan, A., de Haard, H.J., Hultberg, A., Koenders, M., Fooks, A.R., Verrips, T., Wright, E., Saunders, M., Rosseels, V., Weiss, R.A., Vanlandschoot, P.   +11 more
core  

Development of a Nanobody-Based Lateral Flow Immunoassay for Detection of Human Norovirus

, 2016
Human noroviruses are the dominant cause of outbreaks of acute gastroenteritis. These viruses are usually detected by molecular methods, including reverse transcriptase PCR (RT-PCR) and enzyme-linked immunosorbent assay (ELISA).
Anna Koromyslova, Tabatabai, J, Steffen Rameil, Rameil, S, Paul Schnitzler, Julia Tabatabai, Doerflinger, SY, Carlo Farah, Sylvie Y. Doerflinger, Farah, C, Koromyslova, A, Hansman, GS, Peter Sander, Grant S. Hansman, Schnitzler, P, Sander, P   +15 more
core   +1 more source

Performance of pyDock in 8th CAPRI: Energy‐Based Scoring Applied to Docking and AlphaFold Models

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT The 8th CAPRI edition has shown a significant evolution in the field of protein–protein complex structure prediction. We have participated in all 11 targets proposed in this edition, involving domain‐domain, protein–protein, protein‐peptide, and protein‐DNA interactions, including homo‐ and hetero‐meric interfaces.
Luis Angel Rodríguez‐Lumbreras, Mireia Rosell, Miguel Romero‐Durana, Víctor Monteagudo, Fabian Glaser, Juan Fernandez‐Recio   +5 more
wiley   +1 more source

MT3.1: In vitro development and validation of a multivalent nanobody for the selective recognition of pathological tau aggregates

openI nanobodies sono anticorpi formati dalla sola regione variabile della catena pesante, sono delle strutture versatili, stabili e rappresentano una delle innovazioni più promettenti nel campo delle biotecnologie.
PAGIN, MADDALENA
core  

A Versatile Multiplexed Immunofluorescence Strategy for Efficient, Host‐Independent, and Scalable Spatial Protein Profiling

Small Methods, EarlyView.
Crowding‐enhanced multiplex immunofluorescence (umIF) provides a versatile strategy for scalable spatial profiling in FFPE tissues. By improving labeling efficiency across direct, one‐step, and two‐step IF formats, umIF enables host‐species‐independent use of primary antibodies and antibody–nanobody complexes, detection of weak targets, and multicycle ...
Phuong Nguyen, Hongqiang Ma, Dimitrios S. Gotsis, Maomao Chen, Chaojie Zhang, Tushar Talukder Showrav, Marc Schwartz, Brenda Diergaarde, Robert E. Schoen, Rhonda M. Brand, Hua Zhang, Yang Liu   +11 more
wiley   +1 more source

Recent Advances in Photocatalyst‐Driven Protein Labeling and Proximity Mapping

The Chemical Record, EarlyView.
Photocatalyst‐driven protein labeling and proximity mapping have rapidly advanced as powerful strategies for spatiotemporal control in complex biological environments. This review integrates recent developments across single‐electron transfer and energy transfer‐based mechanisms and highlights how catalyst design, reactive intermediates, and diffusion ...
Shinichi Sato, Kazuki Miura, Juki Nakao, Hiroyuki Nakamura   +3 more
wiley   +1 more source