Results 1 to 10 of about 41,395 (219)
Discrete analysis of camelid variable domains: sequences, structures, and in-silico structure prediction [PDF]
PeerJ, 2020 Antigen binding by antibodies requires precise orientation of the complementarity- determining region (CDR) loops in the variable domain to establish the correct contact surface.
Akhila Melarkode Vattekatte +9 more
doaj +2 more sources
Increasing the melting temperature of VHH with the in silico free energy score
Scientific Reports, 2023 VHH, the antigen-binding fragment of a heavy chain-only antibody, is a useful component of antibody-based therapeutics. Thermal stability, represented by the melting temperature (Tm), is one of the key factors affecting the developability of antibody ...
Y. Tomimoto, R. Yamazaki, H. Shirai
semanticscholar +1 more source
Frontiers in Immunology, 2017 The activity of tumor necrosis factor (TNF), a cytokine involved in inflammatory pathologies, can be inhibited by antibodies or trap molecules. Herein, llama-derived variable heavy-chain domains of heavy-chain antibody (VHH, also called Nanobodies™) were
Els Beirnaert +14 more
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VHHs as tools for therapeutic protein delivery to the central nervous system
Fluids and Barriers of the CNS, 2022 Background The blood brain barrier (BBB) limits the therapeutic perspective for central nervous system (CNS) disorders. Previously we found an anti-mouse transferrin receptor (TfR) VHH (Nb62) that was able to deliver a biologically active neuropeptide ...
Yessica Wouters +5 more
doaj +1 more source
Protein Expression and Purification, 2023 Antibody-mimetic drug conjugate (AMDC) is a cancer cell-targeted drug delivery system based on the non-covalent binding of mutated streptavidin and modified biotin, namely Cupid and Psyche. However, the development of AMDCs is hampered by difficulties in
Juri Sakata +21 more
semanticscholar +1 more source
Lactobacilli as a Vector for Delivery of Nanobodies against Norovirus Infection
Pharmaceutics, 2022 Passive administration of neutralizing antibodies (Abs) is an attractive strategy for the control of gastrointestinal infections. However, an unanswered practical concern is the need to assure the stability of sufficient amounts of orally administered ...
Yoshikazu Yuki +8 more
doaj +1 more source
Scientific Reports, 2018 Nucleolin arises as a relevant target for cancer therapy, as it is overexpressed at the surface of cancer and angiogenic endothelial cells thus enabling a dual cellular targeting strategy.
Sofia Romano +4 more
doaj +1 more source
Applied Microbiology and Biotechnology, 2023 Acinetobacter baumannii is a Gram-negative bacterial pathogen that exhibits high intrinsic resistance to antimicrobials, with treatment often requiring the use of last-resort antibiotics.
Eric K Lei +9 more
semanticscholar +1 more source
Large-Scale Production of Anti-RNase A VHH Expressed in pyrG Auxotrophic Aspergillus oryzae
Current Issues in Molecular Biology, 2023 Nanobodies, also referred to as VHH antibodies, are the smallest fragments of naturally produced camelid antibodies and are ideal affinity reagents due to their remarkable properties.
E. Karaman +3 more
semanticscholar +1 more source
Development of potent and effective synthetic SARS-CoV-2 neutralizing nanobodies
mAbs, 2021 The respiratory virus responsible for coronavirus disease 2019 (COVID-19), severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has affected nearly every aspect of life worldwide, claiming the lives of over 3.9 million people globally, at the ...
Maxwell A. Stefan +15 more
doaj +1 more source