Results 1 to 10 of about 665,601 (301)

Single-domain antibody-based protein degrader for synucleinopathies [PDF]

Molecular Neurodegeneration
Synucleinopathies are a group of neurodegenerative diseases characterized by the accumulation of α-synuclein (α-syn) in the brain, leading to motor and neuropsychiatric symptoms.
Yixiang Jiang, Yan Lin, Amber M. Tetlow, Ruimin Pan, Changyi Ji, Xiang-Peng Kong, Erin E. Congdon, Einar M. Sigurdsson   +7 more
doaj   +5 more sources

Development of a Chicken Immunoglobulin Heavy Chain Variable Region (VH) Single-Domain Antibody (sdAb) Against Calsequestrin (CSQ) and Its Application [PDF]

Antibodies
Background/Objectives: Calsequestrin (CSQ) is a calcium-binding protein that is highly soluble and can serve as a solubility-enhancing fusion tag in recombinant protein expression.
Sun Lee, Seoryeong Park, Hyunji Yang, Geummi Cho, Seung Youn Lee, Donggeun Lee, Nara Tae, Dae Hee Kim, Junho Chung   +8 more
doaj   +3 more sources

Bglbrick strategy for the construction of single domain antibody fusions

Heliyon, 2017
Single domain antibodies, recombinantly expressed variable domains derived from camelid heavy chain antibodies, are often expressed as multimers for detection and therapeutic applications.
Ellen R. Goldman, Aeris Broussard, George P. Anderson, Jinny L. Liu   +3 more
doaj   +2 more sources

Single domain antibody multimers confer protection against rabies infection. [PDF]

PLoS ONE, 2013
Post-exposure prophylactic (PEP) neutralizing antibodies against Rabies are the most effective way to prevent infection-related fatality. The outer envelope glycoprotein of the Rabies virus (RABV) is the most significant surface antigen for generating ...
Bhargavi M Boruah, Dawei Liu, Duan Ye, Tie-Jun Gu, Chun-Lai Jiang, Mingsheng Qu, Edward Wright, Wei Wang, Wen He, Changzhen Liu, Bin Gao   +10 more
doaj   +2 more sources

Conjugation of biotin-coated luminescent quantum dots with single domain antibody-rhizavidin fusions

Biotechnology Reports, 2016
Straightforward and effective methods are required for the bioconjugation of proteins to surfaces and particles. Previously we demonstrated that the fusion of a single domain antibody with the biotin binding molecule rhizavidin provided a facile method ...
Jinny L. Liu, Scott A. Walper, Kendrick B. Turner, Audrey Brozozog Lee, Igor L. Medintz, Kimihiro Susumu, Eunkeu Oh, Dan Zabetakis, Ellen R. Goldman, George P. Anderson   +9 more
doaj   +2 more sources

Single-domain antibody screening by is PLA-seq [PDF]

Life Science Alliance, 2022
This study describes a high-sensitive, high-throughput single-domain antibody library screening approach, which is applicable for any given interested protein at single-cell resolution by is PLA-seq.
Yueyuan Yin, Fei Yan, Ruimin Zhou, Mingchen Li, Jinyi Ma, Zhe Liu, Zhenyi Ma   +6 more
doaj   +3 more sources

mRNA Encoding a Bispecific Single Domain Antibody Construct Protects against Influenza A Virus Infection in Mice [PDF]

Molecular Therapy: Nucleic Acids, 2020
To date, mRNA-based biologics have mainly been developed for prophylactic and therapeutic vaccination to combat infectious diseases or cancer. In the past years, optimization of the characteristics of in vitro transcribed mRNA has led to significant ...
Lien Van Hoecke, Rein Verbeke, Dorien De Vlieger, Heleen Dewitte, Kenny Roose, Sharon Van Nevel, Olga Krysko, Claus Bachert, Bert Schepens, Ine Lentacker, Xavier Saelens   +10 more
doaj   +2 more sources

Biotechnological applications of recombinant single-domain antibody fragments [PDF]

Microbial Cell Factories, 2011
Background Single-domain antibody fragments possess structural features, such as a small dimension, an elevated stability, and the singularity of recognizing epitopes non-accessible for conventional antibodies that make them interesting for several ...
de Marco Ario
doaj   +2 more sources

Intracellular Antibody Capture (IAC) Methods for Single Domain Antibodies

, 2012
Intracellular single domain antibodies are recombinant proteins, comprising one variable region domain fragment, that bind specifically to intracellular molecules and can interfere with their particular functions within various cellular compartments.
Tanaka, T, Rabbitts, T
openaire   +4 more sources

An Inside Job: Applications of Intracellular Single Domain Antibodies [PDF]

Biomolecules, 2020
Sera of camelid species contain a special kind of antibody that consists only of heavy chains. The variable antigen binding domain of these heavy chain antibodies can be expressed as a separate entity, called a single domain antibody that is ...
Eline Soetens, Marlies Ballegeer, Xavier Saelens   +2 more
doaj   +4 more sources