Bglbrick strategy for the construction of single domain antibody fusions
Heliyon, 2017 Single domain antibodies, recombinantly expressed variable domains derived from camelid heavy chain antibodies, are often expressed as multimers for detection and therapeutic applications.
Ellen R. Goldman +3 more
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Single domain antibody multimers confer protection against rabies infection. [PDF]
PLoS ONE, 2013 Post-exposure prophylactic (PEP) neutralizing antibodies against Rabies are the most effective way to prevent infection-related fatality. The outer envelope glycoprotein of the Rabies virus (RABV) is the most significant surface antigen for generating ...
Bhargavi M Boruah +10 more
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Conjugation of biotin-coated luminescent quantum dots with single domain antibody-rhizavidin fusions [PDF]
Biotechnology Reports, 2016 Straightforward and effective methods are required for the bioconjugation of proteins to surfaces and particles. Previously we demonstrated that the fusion of a single domain antibody with the biotin binding molecule rhizavidin provided a facile method ...
Jinny L. Liu +9 more
doaj +2 more sources
mRNA Encoding a Bispecific Single Domain Antibody Construct Protects against Influenza A Virus Infection in Mice [PDF]
Molecular Therapy: Nucleic Acids, 2020 To date, mRNA-based biologics have mainly been developed for prophylactic and therapeutic vaccination to combat infectious diseases or cancer. In the past years, optimization of the characteristics of in vitro transcribed mRNA has led to significant ...
Lien Van Hoecke +10 more
doaj +2 more sources
Biotechnological applications of recombinant single-domain antibody fragments [PDF]
Microbial Cell Factories, 2011 Background Single-domain antibody fragments possess structural features, such as a small dimension, an elevated stability, and the singularity of recognizing epitopes non-accessible for conventional antibodies that make them interesting for several ...
de Marco Ario
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Intracellular Antibody Capture (IAC) Methods for Single Domain Antibodies
, 2012 Intracellular single domain antibodies are recombinant proteins, comprising one variable region domain fragment, that bind specifically to intracellular molecules and can interfere with their particular functions within various cellular compartments.
Tanaka, T, Rabbitts, T
openaire +4 more sources
Stabilization of a Broadly Neutralizing Anti-Chikungunya Virus Single Domain Antibody
Frontiers in Medicine, 2021 A single domain antibody (clone CC3) previously found to neutralize a vaccine strain of the chikungunya virus (PRNT50 = 2. 5 ng/mL) was found to be broadly neutralizing.
Jinny L. Liu +9 more
doaj +1 more source
Single Domain Antibody application in bacterial infection diagnosis and neutralization
Frontiers in Immunology, 2022 Increasing antibiotic resistance to bacterial infections causes a serious threat to human health. Efficient detection and treatment strategies are the keys to preventing and reducing bacterial infections. Due to the high affinity and antigen specificity,
Qian Qin +11 more
doaj +1 more source
Single‐Domain Antibodies and Their Utility
Current Protocols in Immunology, 2013 AbstractEngineered monoclonal antibody fragments have gained market attention due to their versatility and tailor‐made potential and are now considered to be an important part of future immunobiotherapeutics. Single‐domain antibodies (sdAbs), also known as nanobodies, are derived from VHHs [variable domains (V) of heavy‐chain‐only antibodies (HCAb)] of
Baral, Toya Nath +2 more
openaire +4 more sources
Enhancement of toxin- and virus-neutralizing capacity of single-domain antibody fragments by N-glycosylation [PDF]
, 2009 Single-domain antibody fragments (VHHs) have several beneficial properties as compared to conventional antibody fragments. However, their small size complicates their toxin- and virus-neutralizing capacity.
Fijten, H.P.D. +5 more
core +1 more source