Results 1 to 10 of about 691 (170)

Intraocular Penetration of a vNAR: In Vivo and In Vitro VEGF165 Neutralization [PDF]

Marine Drugs, 2018
Variable new antigen receptor domain (vNAR) antibodies are novel, naturally occurring antibodies that can be isolated from naïve, immune or synthetic shark libraries. These molecules are very interesting to the biotechnology and pharmaceutical industries
Tanya A. Camacho-Villegas, María Teresa Mata-González, Walter García-Ubbelohd, Linda Núñez-García, Carolina Elosua, Jorge F. Paniagua-Solis, Alexei F. Licea-Navarro   +6 more
doaj   +6 more sources

VNAR development through antigen immunization of Japanese topeshark (Hemitriakis japanica) [PDF]

Frontiers in Bioengineering and Biotechnology, 2023
The VNAR (Variable New Antigen Receptor) is the smallest single-domain antibody derived from the variable domain of IgNAR of cartilaginous fishes. Despite its biomedical and diagnostic potential, research on VNAR has been limited due to the difficulties ...
Hiroyuki Takeda, Tatsuhiko Ozawa, Tatsuhiko Ozawa, Hiroki Zenke, Yoh Ohnuki, Yuri Umeda, Wei Zhou, Honoka Tomoda, Akihiko Takechi, Kimiyoshi Narita, Takaaki Shimizu, Takuya Miyakawa, Yuji Ito, Tatsuya Sawasaki   +13 more
doaj   +6 more sources

VNAR: shark single-domain antibodies for the new era of medical biotechnology [PDF]

Frontiers in Immunology
Shark-derived single-domain antibodies, known as VNARs, represent unique and advanced tools in medical biotechnology. Recognized for their small size, simple structure, and exceptional stability, VNARs can access cryptic epitopes that are inaccessible to
Richard A. Olivares-Olivares, Richard A. Olivares-Olivares, Angélica R. Bravo, Angélica R. Bravo, Carlos Garrido-Soto, Carlos Garrido-Soto, Jonatan J. Carvajal, Jonatan J. Carvajal, Augusto Manubens, Mariella Rivas, Mariella Rivas, Carlos Bustamente, Angello Retamal-Díaz, Angello Retamal-Díaz, Angello Retamal-Díaz, Alexis M. Kalergis, Alexis M. Kalergis, Margarita K. Lay, Margarita K. Lay, Margarita K. Lay   +19 more
doaj   +5 more sources

In vitro selection of VNAR antibodies using bioinformatics-based library design and cDNA display [PDF]

Biotechnology Reports
Variable domains of new antigen receptors (VNARs) derived from shark immunoglobulin new antigen receptors (IgNARs) have gained attention as promising single-domain antibody modalities owing to their compact structures, structural stability, and unique ...
Hiroki Anzai, Takuya Terai, Susumu Mitsuyama, Ryo Matsunaga, Kenwa Tamagawa, Issei Yamaguchi, D.P.N. De Silva, Shigehiro Kuraku, Kouhei Tsumoto, Robert E. Campbell, Shuichi Asakawa   +10 more
doaj   +3 more sources

Isolation and Characterization of Targeting-HBsAg VNAR Single Domain Antibodies from Whitespotted Bamboo Sharks (Chiloscyllium plagiosum) [PDF]

Marine Drugs, 2023
Immunoglobulin new antigen receptor (IgNAR) is a naturally occurring antibody that consists of only two heavy chains with two independent variable domains.
Xierui Jiang, Shan Sun, Zengpeng Li, Mingliang Chen   +3 more
doaj   +3 more sources

Theranostic vNAR-Based Immunoconjugates Achieve Selective Intracellular Cisplatin Delivery in Embedded 3D HER2-Positive Breast Cancer In Vitro Model [PDF]

Pharmaceuticals
Background/Objectives: Precise intracellular delivery of chemotherapeutics remains a major challenge in HER2-positive breast cancer, where intratumoral heterogeneity and limited tissue penetration constrain efficacy.
Andrea C. Alfonseca-Ladrón de Guevara, Alejandro Manzanares-Guzmán, Jessica A. Badillo-Mata, Mirna Burciaga-Flores, Pavel H. Lugo-Fabres, Tanya A. Camacho-Villegas   +5 more
doaj   +2 more sources

Novel Approach for Obtaining Variable Domain of New Antigen Receptor with Different Physicochemical Properties from Japanese Topeshark (Hemitriakis japanica) [PDF]

Marine Drugs, 2023
Diverse candidate antibodies are needed to successfully identify therapeutic and diagnostic applications. The variable domain of IgNAR (VNAR), a shark single-domain antibody, has attracted attention owing to its favorable physicochemical properties.
Tomofumi Nakada-Masuta, Hiroyuki Takeda, Kazuhisa Uchida   +2 more
doaj   +2 more sources

First pan-specific vNAR against human TGF-β as a potential therapeutic application: in silico modeling assessment [PDF]

Scientific Reports, 2023
Immunotherapies based on antibody fragments have been developed and applied to human diseases, describing novel antibody formats. The vNAR domains have a potential therapeutic use related to their unique properties.
Mirna Burciaga-Flores, Ana Laura Márquez-Aguirre, Salvador Dueñas, Jahaziel Gasperin-Bulbarela, Alexei F. Licea-Navarro, Tanya A. Camacho-Villegas   +5 more
doaj   +2 more sources

Identification of nurse shark VNAR single-domain antibodies targeting the spike S2 subunit of SARS-CoV-2

FASEB Journal, 2023
Shark VNAR nanobodies targeting the S2 subunit of SARS‐CoV‐2 spike protein were identified from a naïve nurse shark VNAR phage display library by phage panning. The S2A9 VNAR showed the best neutralization activity against the original pseudotyped virus and was further characterized.
Hyung Joon Kwon, Hang Xie, Mingqian Feng
exaly   +2 more sources

Evaluation of variable new antigen receptors (vNARs) as a novel cathepsin S (CTSS) targeting strategy [PDF]

Frontiers in Pharmacology, 2023
Aberrant activity of the cysteine protease Cathepsin S (CTSS) has been implicated across a wide range of pathologies. Notably in cancer, CTSS has been shown to promote tumour progression, primarily through facilitating invasion and migration of tumour ...
P. Smyth, L. Ferguson, J. F. Burrows, R. E. Burden, S. R. Tracey, Ú. M. Herron, M. Kovaleva, R. Williams, A. J. Porter, A. J. Porter, D. B. Longley, C. J. Barelle, C. J. Scott   +12 more
doaj   +2 more sources