VNAR development through antigen immunization of Japanese topeshark (Hemitriakis japanica) [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 The VNAR (Variable New Antigen Receptor) is the smallest single-domain antibody derived from the variable domain of IgNAR of cartilaginous fishes. Despite its biomedical and diagnostic potential, research on VNAR has been limited due to the difficulties ...
Hiroyuki Takeda
exaly +6 more sources
The influence of antibody humanization on shark variable domain (VNAR) binding site ensembles [PDF]
Frontiers in Immunology, 2022 Sharks and other cartilaginous fish produce new antigen receptor (IgNAR) antibodies, as key part of their humoral immune response and are the phylogenetically oldest living organisms that possess an immunoglobulin (Ig)-based adaptive immune system. IgNAR
Franz Waibl +2 more
exaly +6 more sources
Allosteric inhibition of Aurora-A kinase by a synthetic vNAR domain [PDF]
Open Biology, 2016 The vast majority of clinically approved protein kinase inhibitors target the ATP-binding pocket directly. Consequently, many inhibitors have broad selectivity profiles and most have significant off-target effects.
Selena G. Burgess +6 more
doaj +6 more sources
Construction and application of a large capacity VNAR library from the whitespotted bamboo shark (Chiloscyllium playgiosum) [PDF]
Acta Pharmaceutica Sinica BFifty whitespotted bamboo sharks (Chiloscyllium playgiosum) of both sexes were used to establish a large capacity variable domain of the new antigen receptor (VNAR) library with a total capacity of over 109 colony-forming units (CFU).
ChuanWei CHEN, Ming-Wei Wang
exaly +4 more sources
Isolation and Characterization of Targeting-HBsAg VNAR Single Domain Antibodies from Whitespotted Bamboo Sharks (Chiloscyllium plagiosum) [PDF]
Marine Drugs, 2023 Immunoglobulin new antigen receptor (IgNAR) is a naturally occurring antibody that consists of only two heavy chains with two independent variable domains.
Xierui Jiang +3 more
doaj +4 more sources
CDR3 Variants of the TXB2 Shuttle with Increased TfR1 Association Rate and Enhanced Brain Penetration [PDF]
Pharmaceutics, 2023 Since the delivery of biologic drugs to the brain is greatly hampered by the existence of the blood–brain barrier (BBB), brain shuttles are being developed to enhance therapeutic efficacy.
Pawel Stocki +11 more
doaj +6 more sources
Expression Analysis of Heavy-Chain-Only Antibodies in Cloudy Catshark and Japanese Bullhead Shark [PDF]
Marine DrugsHeavy chain-only antibodies in sharks are called immunoglobulin new antigen receptors (IgNAR), consisting of one variable region (VNAR) and five constant regions (C1-C5).
Reo Uemura +8 more
doaj +5 more sources
Novel Approach for Obtaining Variable Domain of New Antigen Receptor with Different Physicochemical Properties from Japanese Topeshark (Hemitriakis japanica) [PDF]
Marine Drugs, 2023 Diverse candidate antibodies are needed to successfully identify therapeutic and diagnostic applications. The variable domain of IgNAR (VNAR), a shark single-domain antibody, has attracted attention owing to its favorable physicochemical properties.
Tomofumi Nakada-Masuta +2 more
doaj +2 more sources
First pan-specific vNAR against human TGF-β as a potential therapeutic application: in silico modeling assessment [PDF]
Scientific Reports, 2023 Immunotherapies based on antibody fragments have been developed and applied to human diseases, describing novel antibody formats. The vNAR domains have a potential therapeutic use related to their unique properties.
Mirna Burciaga-Flores +5 more
doaj +2 more sources
Mechanisms of SARS-CoV-2 neutralization by shark variable new antigen receptors elucidated through X-ray crystallography [PDF]
Nature Communications, 2021 Shark antibodies (Variable New Antigen Receptors, VNARs) are the smallest naturally occurring antibody fragments. Here, the authors screen a VNAR phage display library against the SARS-CoV2 receptor binding domain (RBD) and identify VNARs that neutralize
Obinna C. Ubah +20 more
doaj +2 more sources