Results 41 to 50 of about 487 (135)

A Class of Shark‐Derived Single‐Domain Antibodies can Broadly Neutralize SARS‐Related Coronaviruses and the Structural Basis of Neutralization and Omicron Escape

Small Methods, Volume 6, Issue 7, July 20, 2022., 2022
Shark‐derived vnarbodies 20G6 and 17F6 broadly neutralize SARS‐CoV‐2 Wuhan, Alpha, Beta, Kappa, Delta, Delta‐plus, Lambda variants, and sarbecovirus coronaviruses, but are unexpectedly ineffective for Omicron. Intranasal administration of 20G6 confers effective preventive and therapeutic effects in mice infected with SARS‐CoV‐2 Wuhan and Beta variant ...
Bo Feng, Zhilong Chen, Jing Sun, Tingting Xu, Qian Wang, Haisu Yi, Xuefeng Niu, Jiabin Zhu, Mengzhu Fan, Ruitian Hou, Ying Shao, Sihui Huang, Cuiyun Li, Peiyu Hu, Pingqian Zheng, Ping He, Jia Luo, Qihong Yan, Xiaoli Xiong, Jinsong Liu, Jincun Zhao, Ling Chen   +21 more
wiley   +1 more source

Detection of genome‐edited and endogenously expressed G protein‐coupled receptors

The FEBS Journal, Volume 288, Issue 8, Page 2585-2601, April 2021., 2021
G protein‐coupled receptors (GPCRs) are the largest family of membrane receptors and major targets for FDA‐approved drugs. Quantifying GPCR expression and ligand binding characteristics in different cell types is important for drug discovery. The advent of genome editing and developments in fluorescent ligand design offers exciting possibilities to ...
Mark Soave, Leigh A. Stoddart, Carl W. White, Laura E. Kilpatrick, Joëlle Goulding, Stephen J. Briddon, Stephen J. Hill   +6 more
wiley   +1 more source

Single domain shark VNAR antibodies neutralize SARS-CoV-2 infection in vitro [PDF]

The FASEB Journal, 2021
Abstract Single domain shark VNAR antibodies can offer a viable alternative to conventional Ig-based monoclonal antibodies in treating COVID-19 disease during the current pandemic. Here we report the identification of neutralizing single domain VNAR antibodies selected against the SARS-CoV-2 spike protein derived from
Aziz, Gauhar, Cyril V, Privezentzev, Mykhaylo, Demydchuk, Tanja, Gerlza, Julia, Rieger, Andreas J, Kungl, Frank S, Walsh, J Lynn, Rutkowski, Pawel, Stocki   +8 more
openaire   +3 more sources

Shark Antibody Variable Domains Rigidify Upon Affinity Maturation—Understanding the Potential of Shark Immunoglobulins as Therapeutics

Frontiers in Molecular Biosciences, 2021
Sharks and other cartilaginous fish are the phylogenetically oldest living organisms that have antibodies as part of their adaptive immune system.
Monica L. Fernández-Quintero, Clarissa A. Seidler, Patrick K. Quoika, Klaus R. Liedl   +3 more
doaj   +1 more source

Brain delivery of biologics using a cross‐species reactive transferrin receptor 1 VNAR shuttle

The FASEB Journal, Volume 34, Issue 10, Page 13272-13283, October 2020., 2020
Abstract Transferrin receptor 1 (TfR1) mediated transcytosis is an attractive strategy to enhance brain uptake of protein drugs, but translation remains a challenge. Here, a single domain shark antibody VNAR fragment (TXB2) with similar affinity to murine and human TfR1 was used to shuttle protein cargo into the brain. TXB2 was fused to a human IgG1 Fc
Dag Sehlin, Pawel Stocki, Tobias Gustavsson, Greta Hultqvist, Frank S. Walsh, J. Lynn Rutkowski, Stina Syvänen   +6 more
wiley   +1 more source

Shark New Antigen Receptor (IgNAR): Structure, Characteristics and Potential Biomedical Applications

Cells, 2021
Shark is a cartilaginous fish that produces new antigen receptor (IgNAR) antibodies. This antibody is identified with a similar human heavy chain but dissimilar sequences.
Salma Nassor Juma, Xiaoxia Gong, Sujie Hu, Zhengbing Lv, Jianzhong Shao, Lili Liu, Guiqian Chen   +6 more
doaj   +1 more source

Functionalization of polymeric nanoparticles with targeting VNAR ligands using vinyl sulfone conjugation

Journal of Materials Chemistry B, 2023
Vinyl sulfone (VS) functionalization – An innovative conjugation approach for preparation of variable new antigen receptor (VNAR) targeted polymeric nanoparticles.
Adam Leach, Marie Finnegan, Mariana S. Machado, Laura Ferguson, John Steven, Peter Smyth, Andrew Porter, Caroline Barelle, Efrosyni Themistou, Christopher J. Scott   +9 more
openaire   +4 more sources

Shark Variable New Antigen Receptor (VNAR) Single Domain Antibody Fragments: Stability and Diagnostic Applications

Antibodies, 2013
The single variable new antigen receptor domain antibody fragments (VNARs) derived from shark immunoglobulin new antigen receptor antibodies (IgNARs) represent some of the smallest known immunoglobulin-based protein scaffolds.
Stewart Nuttall, Matthew A. Perugini, Iveth González, Joanne L. Casey, Abdulmonem Sanalla, Miles Barraclough, Con Dogovski, Julie Angerosa, Kathy Parisi, Olan Dolezal, Katherine Griffiths, Michael Foley   +11 more
doaj   +1 more source

Mechanisms of SARS-CoV-2 neutralization by shark variable new antigen receptors elucidated through X-ray crystallography

Nature Communications, 2021
Shark antibodies (Variable New Antigen Receptors, VNARs) are the smallest naturally occurring antibody fragments. Here, the authors screen a VNAR phage display library against the SARS-CoV2 receptor binding domain (RBD) and identify VNARs that neutralize
Obinna C. Ubah, Eric W. Lake, Gihan S. Gunaratne, Joseph P. Gallant, Marie Fernie, Austin J. Robertson, Jonathan S. Marchant, Tyler D. Bold, Ryan A. Langlois, William E. Matchett, Joshua M. Thiede, Ke Shi, Lulu Yin, Nicholas H. Moeller, Surajit Banerjee, Laura Ferguson, Marina Kovaleva, Andrew J. Porter, Hideki Aihara, Aaron M. LeBeau, Caroline J. Barelle   +20 more
doaj   +1 more source

Isolation and characterization of malaria PfHRP2 specific VNAR antibody fragments from immunized shark phage display library

Malaria Journal, 2018
Background Malaria rapid diagnostic tests (RDTs) represent an important antibody based immunoassay platform. Unfortunately, conventional monoclonal antibodies are subject to degradation shortening shelf lives of RDTs. The variable region of the receptor (
Chiuan Herng Leow, Katja Fischer, Chiuan Yee Leow, Katleen Braet, Qin Cheng, James McCarthy   +5 more
doaj   +1 more source