Results 61 to 70 of about 485 (131)

Escherichia coli surface display for the selection of nanobodies

Microbial Biotechnology, Volume 10, Issue 6, Page 1468-1484, November 2017., 2017
Libraries of VHH genes encoding nanobodies have been traditionally cloned in phage display or yeast display systems. This review briefly describes these conventional approaches and focuses on the distinct properties of an E. coli display system developed in our laboratory, which combines the benefits of both phage display and yeast display systems ...
Valencio Salema, Luis Ángel Fernández
wiley   +1 more source

Marine-derived microbes and molecules for drug discovery

Inflammation and Regeneration, 2022
Increasing attention has been paid to marine-derived biomolecules as sources of therapeutics for autoimmune diseases. Nagasaki Prefecture has many islands and is surrounded by seas, straits, gulfs, bays, and coves, giving it the second longest coastline ...
Yoshimasa Tanaka, Megumi Nishikawa, Kaho Kamisaki, Saki Hachiya, Moeka Nakamura, Takahiro Kuwazuru, Susumu Tanimura, Kiyoshi Soyano, Kohsuke Takeda   +8 more
doaj   +1 more source

Synthesis and Characterization of TiO2 Modified with Polystyrene and Poly(3‐chloro‐2‐hydroxypropyl Methacrylate) as Adsorbents for the Solid Phase Extraction of Organophosphorus Pesticides

Journal of Chemistry, Volume 2016, Issue 1, 2016., 2016
Novel hybrid TiO2 particles were developed and assessed as an adsorbent for solid phase extraction (SPE) of organophosphorus pesticides (fensulfothion, parathion methyl, coumaphos, and diazinon) from spiked water. The sol‐gel method was used to synthesize TiO2 particles, which were coated with free‐radical polystyrene (PS) and poly(3‐chloro‐2 ...
Enrique Alejo-Molina, A. Rafael Vilchis-Néstor, David Muñoz-Rodríguez, Cristian Carrera-Figueiras, Jean-Marie Nedelec   +4 more
wiley   +1 more source

Study of the Performance of the Organic Extracts of Chenopodium ambrosioides for Ag Nanoparticle Synthesis

Journal of Nanomaterials, Volume 2016, Issue 1, 2016., 2016
There are many ways to obtain metal nanoparticles: biological, physical, and chemical ways and combinations of these approaches. Synthesis assisted with plant extracts has been widely documented. However, one issue that is under discussion refers to the metabolites responsible for reduction and stabilization that confine nanoparticle growth and prevent
Luis M. Carrillo-López, Ramón M. Soto-Hernández, Hilda A. Zavaleta-Mancera, Alfredo R. Vilchis-Néstor, Xuping Sun   +4 more
wiley   +1 more source

Facile Solventless Synthesis of a Nylon‐6,6/Silver Nanoparticles Composite and Its XPS Study

International Journal of Polymer Science, Volume 2013, Issue 1, 2013., 2013
Silver nanoparticles were synthesized and supported on thin nylon membranes by means of a simple method of impregnation and chemical reduction of Ag ions at ambient conditions. Particles of less than 10 nm were obtained using this methodology, in which the nylon fibers behave as constrained nanoreactors.
Raúl A. Morales-Luckie, Víctor Sánchez-Mendieta, Oscar Olea-Mejia, Alfredo R. Vilchis-Nestor, Gustavo López-Téllez, Víctor Varela-Guerrero, L. Huerta, Jesús Arenas-Alatorre, Carmina Menchaca-Campos   +8 more
wiley   +1 more source

Novel, Anti-hTNF-α Variable New Antigen Receptor Formats with Enhanced Neutralizing Potency and Multifunctionality, Generated for Therapeutic Development

Frontiers in Immunology, 2017
The management of chronic inflammatory diseases, such as inflammatory bowel disease, psoriasis, and rheumatoid arthritis has significantly improved over the last decade with the clinical availability of anti-TNF-α biologics.
Obinna C. Ubah, John Steven, Marina Kovaleva, Laura Ferguson, Charlotte Barelle, Andrew J. R. Porter, Andrew J. R. Porter, Caroline J. Barelle   +7 more
doaj   +1 more source

From Multi‐Omics to Visualization and Beyond: Bridging Micro and Macro Insights in CAR‐T Cell Therapy

Advanced Science, Volume 12, Issue 20, May 29, 2025.
This review explores how multi‐omics, visualization technologies, and AI drive CAR‐T therapy from the micro to the macro level, from preclinical studies to clinical applications. The convergence of these approaches provides deeper insight into CAR‐T cell mechanisms, aids in structural optimization, and significantly enhances treatment efficacy and ...
Yuting Gong, Peng Fei, Yicheng Zhang, Yang Xu, Jia Wei   +4 more
wiley   +1 more source

Study Design of the Microcirculatory Shock Occurrence in Acutely Ill Patients (microSOAP): an International Multicenter Observational Study of Sublingual Microcirculatory Alterations in Intensive Care Patients

Critical Care Research and Practice, Volume 2012, Issue 1, 2012., 2012
Objective. Sublingual microcirculatory alterations are associated with an adverse prognosis in several critical illness subgroups. Up to now, single‐center studies have reported on sublingual microcirculatory alterations in ICU patient subgroups, but an extensive evaluation of the prevalence of these alterations is lacking.
Namkje A. R. Vellinga, E. Christiaan Boerma, Matty Koopmans, Abele Donati, Arnaldo Dubin, Nathan I. Shapiro, Rupert M. Pearse, Jan Bakker, Can Ince, Michael Piagnerelli   +9 more
wiley   +1 more source

Construction and evaluation of a synthetic VNAR library for targeted antigen screening

Protein Science
Abstract Sharks produce a unique antibody type, whose variable domain (VNAR) is the smallest known antibody fragment. VNARs possess high affinity, stability, and tissue penetration, making them attractive for biomedical applications.
Zu‐Ying Liu, Wen‐Hui Lei, Hong‐Yan Wan, Fei‐Yan Tao, Xiao‐Min Tao, Tengchuan Jin, Min‐Jie Cao, Duanquan Lin, Le‐Chang Sun, Ling‐Jing Zhang, Yu‐Lei Chen   +10 more
openaire   +2 more sources

Single domain antibody: Development and application in biotechnology and biopharma

Immunological Reviews, Volume 328, Issue 1, Page 98-112, November 2024.
Summary Heavy‐chain antibodies (HCAbs) are a unique type of antibodies devoid of light chains, and comprised of two heavy chains‐only that recognize their cognate antigen by virtue of a single variable domain also referred to as VHH, single domain antibody (sdAb), or nanobody (Nb).
Ting Yu, Fang Zheng, Wenbo He, Serge Muyldermans, Yurong Wen   +4 more
wiley   +1 more source