Results 51 to 60 of about 485 (131)

A novel PD-L1-targeted shark VNAR single-domain-based CAR-T cell strategy for treating breast cancer and liver cancer

Molecular Therapy: Oncolytics, 2022
Chimeric antigen receptor (CAR)-T cell therapy shows excellent potency against hematological malignancies, but it remains challenging to treat solid tumors, mainly because of a lack of appropriate antigenic targets and an immunosuppressive tumor ...
Dan Li, Hejiao English, Jessica Hong, Tianyuzhou Liang, Glenn Merlino, Chi-Ping Day, Mitchell Ho   +6 more
doaj   +1 more source

Screening and Characterization of Shark-Derived VNARs against SARS-CoV-2 Spike RBD Protein. [PDF]

Int J Mol Sci, 2022
The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein is the major target for antibody therapeutics. Shark-derived variable domains of new antigen receptors (VNARs) are the smallest antibody fragments with flexible paratopes that can recognize protein motifs inaccessible to classical antibodies. This study reported four VNARs binders (JM-2,
Chen YL, Lin JJ, Ma H, Zhong N, Xie XX, Yang Y, Zheng P, Zhang LJ, Jin T, Cao MJ.   +9 more
europepmc   +3 more sources

Improving the pharmacokinetic properties of biologics by fusion to an anti-HSA shark VNAR domain [PDF]

mAbs, 2012
Advances in recombinant antibody technology and protein engineering have provided the opportunity to reduce antibodies to their smallest binding domain components and have concomitantly driven the requirement for devising strategies to increase serum half-life to optimise drug exposure, thereby increasing therapeutic efficacy. In this study, we adopted
Mischa R, Müller, Kenneth, Saunders, Christopher, Grace, Macy, Jin, Nicole, Piche-Nicholas, John, Steven, Ronan, O'Dwyer, Leeying, Wu, Lam, Khetemenee, Yulia, Vugmeyster, Timothy P, Hickling, Lioudmila, Tchistiakova, Stephane, Olland, Davinder, Gill, Allan, Jensen, Caroline J, Barelle   +15 more
openaire   +2 more sources

Shark Variable New Antigen Receptor (VNAR) Single Domain Antibody Fragments: Stability and Diagnostic Applications

Antibodies, 2013
The single variable new antigen receptor domain antibody fragments (VNARs) derived from shark immunoglobulin new antigen receptor antibodies (IgNARs) represent some of the smallest known immunoglobulin-based protein scaffolds.
Stewart Nuttall, Matthew A. Perugini, Iveth González, Joanne L. Casey, Abdulmonem Sanalla, Miles Barraclough, Con Dogovski, Julie Angerosa, Kathy Parisi, Olan Dolezal, Katherine Griffiths, Michael Foley   +11 more
doaj   +1 more source

Mechanisms of SARS-CoV-2 neutralization by shark variable new antigen receptors elucidated through X-ray crystallography

Nature Communications, 2021
Shark antibodies (Variable New Antigen Receptors, VNARs) are the smallest naturally occurring antibody fragments. Here, the authors screen a VNAR phage display library against the SARS-CoV2 receptor binding domain (RBD) and identify VNARs that neutralize
Obinna C. Ubah, Eric W. Lake, Gihan S. Gunaratne, Joseph P. Gallant, Marie Fernie, Austin J. Robertson, Jonathan S. Marchant, Tyler D. Bold, Ryan A. Langlois, William E. Matchett, Joshua M. Thiede, Ke Shi, Lulu Yin, Nicholas H. Moeller, Surajit Banerjee, Laura Ferguson, Marina Kovaleva, Andrew J. Porter, Hideki Aihara, Aaron M. LeBeau, Caroline J. Barelle   +20 more
doaj   +1 more source

Isolation and characterization of malaria PfHRP2 specific VNAR antibody fragments from immunized shark phage display library

Malaria Journal, 2018
Background Malaria rapid diagnostic tests (RDTs) represent an important antibody based immunoassay platform. Unfortunately, conventional monoclonal antibodies are subject to degradation shortening shelf lives of RDTs. The variable region of the receptor (
Chiuan Herng Leow, Katja Fischer, Chiuan Yee Leow, Katleen Braet, Qin Cheng, James McCarthy   +5 more
doaj   +1 more source

Shape Tuning of Magnetite Nanoparticles Obtained by Hydrothermal Synthesis: Effect of Temperature

Journal of Nanomaterials, Volume 2019, Issue 1, 2019., 2019
In this work, we present a simple and efficient method for pure phase magnetite (Fe3O4) nanoparticle synthesis. The phase structure, particle shape, and size of the samples were characterized by Raman spectroscopy (Rm), X‐ray diffraction (XRD), scanning electron microscopy (SEM), energy‐dispersive X‐ray (EDS), and transmission electron microscopy (TEM).
Nayely Torres-Gómez, Osvaldo Nava, Liliana Argueta-Figueroa, René García-Contreras, Armando Baeza-Barrera, Alfredo R. Vilchis-Nestor, Paulo Cesar Morais   +6 more
wiley   +1 more source

Plasmonic Sensing of Aqueous‐Divalent Metal Ions by Biogenic Gold Nanoparticles

Journal of Nanomaterials, Volume 2019, Issue 1, 2019., 2019
The chemical interaction between biogenic gold nanoparticles (AuNPs) and several metal (II) ions can be regarded as a practical, twofold, colorimetric, and plasmon resonance sensing method for the recognition of some divalent metal ions in aqueous solutions. The green synthesized AuNPs, using Camellia sinensis as a reducing agent, were characterized by
Luisa E. Silva-De Hoyos, Victor Sánchez-Mendieta, Alfredo R. Vilchis-Nestor, Miguel A. Camacho-López, Jésica Trujillo-Reyes, Miguel Avalos-Borja, Ilaria Fratoddi   +6 more
wiley   +1 more source

Therapeutic Potential of Shark Anti-ICOSL VNAR Domains is Exemplified in a Murine Model of Autoimmune Non-Infectious Uveitis

Frontiers in Immunology, 2017
Induced costimulatory ligand (ICOSL) plays an important role in the activation of T cells through its interaction with the inducible costimulator, ICOS. Suppression of full T cell activation can be achieved by blocking this interaction and has been shown
Marina Kovaleva, Katherine Johnson, John Steven, Caroline J. Barelle, Andrew Porter, Andrew Porter   +5 more
doaj   +1 more source

Anti‐ICOSL New Antigen Receptor Domains Inhibit T Cell Proliferation and Reduce the Development of Inflammation in the Collagen‐Induced Mouse Model of Rheumatoid Arthritis

Journal of Immunology Research, Volume 2018, Issue 1, 2018., 2018
Lymphocyte costimulation plays a central role in immunology, inflammation, and immunotherapy. The inducible T cell costimulator (ICOS) is expressed on T cells following peptide: MHC engagement with CD28 costimulation. The interaction of ICOS with its sole ligand, the inducible T cell costimulatory ligand (ICOSL; also known as B7‐related protein‐1 ...
Ronan O’Dwyer, Marina Kovaleva, Jiquan Zhang, John Steven, Emma Cummins, Deborah Luxenberg, Alfredo Darmanin-Sheehan, Miguel F. Carvalho, Matthew Whitters, Kenneth Saunders, Caroline J. Barelle, Ilaria Roato   +11 more
wiley   +1 more source