Results 31 to 40 of about 38,752 (185)

Mucosal nanobody IgA as inhalable and affordable prophylactic and therapeutic treatment against SARS-CoV-2 and emerging variants

Frontiers in Immunology, 2022
Anti-COVID antibody therapeutics have been developed but not widely used due to their high cost and escape of neutralization from the emerging variants.
Qi Li, Fiachra Humphries, Roxie C. Girardin, Aaron Wallace, Monir Ejemel, Alla Amcheslavsky, Conor T. McMahon, Zachary A. Schiller, Zepei Ma, John Cruz, Alan P. Dupuis, Anne F. Payne, Arooma Maryam, Nese Kurt Yilmaz, Kathleen A. McDonough, Brian G. Pierce, Celia A. Schiffer, Andrew C. Kruse, Mark S. Klempner, Lisa A. Cavacini, Katherine A. Fitzgerald, Yang Wang   +21 more
doaj   +1 more source

Llama-derived single domain antibodies to build multivalent, superpotent and broadened neutralizing anti-viral molecules.

PLoS ONE, 2011
For efficient prevention of viral infections and cross protection, simultaneous targeting of multiple viral epitopes is a powerful strategy. Llama heavy chain antibody fragments (VHH) against the trimeric envelope proteins of Respiratory Syncytial Virus (
Anna Hultberg, Nigel J Temperton, Valérie Rosseels, Mireille Koenders, Maria Gonzalez-Pajuelo, Bert Schepens, Lorena Itatí Ibañez, Peter Vanlandschoot, Joris Schillemans, Michael Saunders, Robin A Weiss, Xavier Saelens, José A Melero, C Theo Verrips, Steven Van Gucht, Hans J de Haard   +15 more
doaj   +1 more source

Versatile Detection of Cellular Protein via Fluorescence Anisotropy

Advanced Science, EarlyView.
Cell Lysate Fluorescence Anisotropy (CFAST) utilizes long lifetime dye‐labeled nanobodies for rapid protein quantification in minimally purified, complex cell lysate. When coupled with cellular thermal shift, CFAST allows high‐throughput detection of endogenous protein‐small molecule engagement, facilitating the discovery of novel binders for ...
Qing Tang, Yuan‐Ping Wei, Ricky Ruiqi Ma, Na Wei, Tammy Zihan Zhou, Hilary Kung‐Yu Ho, Zafar Iqbal Bhat, Iqra Ishrat, Xiaoyu Li, Judy Wai Ping Yam, Yung Hou Wong, Qiankun Wang, Justin L. Tan   +12 more
wiley   +1 more source

Anti-idiotypic VHH mediated environmentally friendly immunoassay for citrinin without mycotoxin

Food and Agricultural Immunology, 2020
Competitive enzyme-linked immunosorbent assay (ELISA) is the most widely used method for rapidly screening of mycotoxins in contaminated food products; however, it involves the use of toxin-protein conjugates as a competitive antigen and toxin standards ...
Liang Xiong, Xueqin Zhang, Yang Xu, Yanping Li, Deguang Liu, Zhui Tu, Qinghua He   +6 more
doaj   +1 more source

Protective effect of different anti-rabies virus VHH constructs against rabies disease in mice. [PDF]

PLoS ONE, 2014
Rabies virus causes lethal brain infection in about 61000 people per year. Each year, tens of thousands of people receive anti-rabies prophylaxis with plasma-derived immunoglobulins and vaccine soon after exposure. Anti-rabies immunoglobulins are however
Sanne Terryn, Aurélie Francart, Sophie Lamoral, Anna Hultberg, Heidi Rommelaere, Angela Wittelsberger, Filip Callewaert, Thomas Stohr, Kris Meerschaert, Ingrid Ottevaere, Catelijne Stortelers, Peter Vanlandschoot, Michael Kalai, Steven Van Gucht   +13 more
doaj   +1 more source

A Rationally Designed Bovine IgA Fc Scaffold Enhances in planta Accumulation of a VHH-Fc Fusion Without Compromising Binding to Enterohemorrhagic E. coli

Frontiers in Plant Science, 2021
We previously isolated a single domain antibody (VHH) that binds Enterohemorrhagic Escherichia coli (EHEC) with the end-goal being the enteromucosal passive immunization of cattle herds. To improve the yield of a chimeric fusion of the VHH with an IgA Fc,
Adam Chin-Fatt, Adam Chin-Fatt, Reza Saberianfar, Rima Menassa, Rima Menassa   +4 more
doaj   +1 more source

A Generative Neuro‐Symbolic AI for Protein Sequence Design

Advanced Science, EarlyView.
We introduce EffieDes, a neuro‐symbolic framework coupling deep learning‐based fitness landscape parameterization with exact automated reasoning. Unlike greedy sampling, EffieDes identifies sequences that globally optimize fitness while satisfying intricate design constraints.
Marianne Defresne, Delphine Dessaux, Samuel Buchet, Lucie Barthe, Liza Ammar‐Khodja, Bessam Azizi, Valentin Durante, Gianluca Cioci, Simon de Givry, Alain Roussel, Luis F. Garcia‐Alles, Thomas Schiex, Sophie Barbe   +12 more
wiley   +1 more source

A bispecific nanobody approach to leverage the potent and widely applicable tumor cytolytic capacity of Vγ9Vδ2-T cells

OncoImmunology, 2018
Though Vγ9Vδ2-T cells constitute only a small fraction of the total T cell population in human peripheral blood, they play a vital role in tumor defense and are therefore of major interest to explore for cancer immunotherapy.
Renée C. G. de Bruin, John P. Veluchamy, Sinéad M. Lougheed, Famke L. Schneiders, Silvia Lopez-Lastra, Roeland Lameris, Anita G. Stam, Zsolt Sebestyen, Jürgen Kuball, Carla F. M. Molthoff, Erik Hooijberg, Rob C. Roovers, James P. Di Santo, Paul M. P. van Bergen en Henegouwen, Henk M. W. Verheul, Tanja D. de Gruijl, Hans J. van der Vliet   +16 more
doaj   +1 more source

Anticuerpos de cadena única de alpaca para la detección de antígenos de Fasciola hepatica

Revista Peruana de Medicina Experimental y Salud Pública, 2018
Objetivo. Producir anticuerpos recombinantes de cadena única de alpaca que se unan con alta afinidad y especificidad al antígeno excretado-secretado (ES) de Fasciola hepatica para el desarrollo de tecnologías nuevas de diagnóstico de fascioliasis humana ...
Teresa Barreto, Yenisey Alfonso, Pierre Lafaye, Maria del Pilar García Lazaro, L. Agueda Perez, Patricia Herrera-Velit, Jose R. Espinoza   +6 more
doaj   +1 more source

Post-exposure Treatment with Anti-rabies VHH and Vaccine Significantly Improves Protection of Mice from Lethal Rabies Infection. [PDF]

PLoS Neglected Tropical Diseases, 2016
Post-exposure prophylaxis (PEP) against rabies infection consists of a combination of passive immunisation with plasma-derived human or equine immune globulins and active immunisation with vaccine delivered shortly after exposure.
Sanne Terryn, Aurélie Francart, Heidi Rommelaere, Catelijne Stortelers, Steven Van Gucht   +4 more
doaj   +1 more source