Results 51 to 60 of about 42,252 (217)

Protective effect of different anti-rabies virus VHH constructs against rabies disease in mice. [PDF]

PLoS ONE, 2014
Rabies virus causes lethal brain infection in about 61000 people per year. Each year, tens of thousands of people receive anti-rabies prophylaxis with plasma-derived immunoglobulins and vaccine soon after exposure. Anti-rabies immunoglobulins are however
Sanne Terryn, Aurélie Francart, Sophie Lamoral, Anna Hultberg, Heidi Rommelaere, Angela Wittelsberger, Filip Callewaert, Thomas Stohr, Kris Meerschaert, Ingrid Ottevaere, Catelijne Stortelers, Peter Vanlandschoot, Michael Kalai, Steven Van Gucht   +13 more
doaj   +1 more source

A Rationally Designed Bovine IgA Fc Scaffold Enhances in planta Accumulation of a VHH-Fc Fusion Without Compromising Binding to Enterohemorrhagic E. coli

Frontiers in Plant Science, 2021
We previously isolated a single domain antibody (VHH) that binds Enterohemorrhagic Escherichia coli (EHEC) with the end-goal being the enteromucosal passive immunization of cattle herds. To improve the yield of a chimeric fusion of the VHH with an IgA Fc,
Adam Chin-Fatt, Adam Chin-Fatt, Reza Saberianfar, Rima Menassa, Rima Menassa   +4 more
doaj   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Distinct types of VHHs in Alpaca

Frontiers in Immunology
IntroductionVHHs (VH of heavy-chain-only antibodies) represent a unique alternative to Q7 conventional antibodies because of their smaller size, comparable binding affinity and biophysical properties. MethodIn this study, we systematically analyzed VHH NGS sequences from 22 Alpacas and structure data from public database.
Xinhao Wang, Lu Zhang, Yao Zhang, Jiaguo Li, Wenfeng Xu, Weimin Zhu   +5 more
openaire   +3 more sources

Overexpression, purification, characterization, and pathogenicity of Vibrio harveyi hemolysin VHH

, 2006
Vibrio harveyi VHH hemolysin is a putative pathogenicity factor in fish. In this study, the hemolysin gene vhhA was overexpressed in Escherichia coli, and the purified VHH was characterized with regard to pH and temperature profiles, phospholipase ...
Zhang, Xiao-Hua, Sun, Boguang, Li, Yun, Chi, Zhenghao, Chen, Jixiang, Austin, Brian, Zhong, Yingbin, Zhang, Xiao H.   +7 more
core   +1 more source

VHH heterohexamer degradation by intestinal proteases.

, 2023
(A) Western blot of a VHH heterohexameric protein (50 μg/ml) following incubation with extracts of human feces or porcine intestine. Intestinal extracts were added at a 1:50 dilution to serum-free conditioned medium of CHO cells transfected with the ...
Saul Tzipori (37482), Raymond K. Kudej (1916548), Noah Sather (6011624), Michelle Debatis (221427), Justyna J. Jaskiewicz (13803931), Charles B. Shoemaker (189753), Jacqueline M. Tremblay (11914529), Hillary Danz (17457839), Kimberly Gaspie (17457842), Vladimir Vigdorovich (5439809)   +9 more
core   +1 more source

Post-exposure Treatment with Anti-rabies VHH and Vaccine Significantly Improves Protection of Mice from Lethal Rabies Infection. [PDF]

PLoS Neglected Tropical Diseases, 2016
Post-exposure prophylaxis (PEP) against rabies infection consists of a combination of passive immunisation with plasma-derived human or equine immune globulins and active immunisation with vaccine delivered shortly after exposure.
Sanne Terryn, Aurélie Francart, Heidi Rommelaere, Catelijne Stortelers, Steven Van Gucht   +4 more
doaj   +1 more source

Smaller is better: nanobodies meet NMR

FEBS Letters, EarlyView.
Nanobodies are single‐domain antigen‐binding fragments derived from camelid heavy chain antibodies. Their small size, high stability, and exceptional specificity make nanobodies uniquely useful probes for NMR studies of protein dynamics, transient conformational states, and protein–protein interactions.
Oleg Y. Dmitriev
wiley   +1 more source

Isolation of Novel EGFR-Specific VHH Domains [PDF]

SLAS Discovery, 2009
Epidermal growth factor receptor (EGFR) is overexpressed or mutated in a high percentage of tumors. EGFR has long been considered a promising target for cancer diagnostic and therapeutic applications. However, monoclonal antibodies and other large antibody constructs diffuse into tumors slowly, limiting their efficacy. To develop lower molecular weight
Elizabeth B, Gottlin, , Xiangrong Guan, Charles, Pegram, Allen, Cannedy, Michael J, Campa, Edward F, Patz   +5 more
openaire   +2 more sources

A Generative Neuro‐Symbolic AI for Protein Sequence Design

Advanced Science, EarlyView.
We introduce EffieDes, a neuro‐symbolic framework coupling deep learning‐based fitness landscape parameterization with exact automated reasoning. Unlike greedy sampling, EffieDes identifies sequences that globally optimize fitness while satisfying intricate design constraints.
Marianne Defresne, Delphine Dessaux, Samuel Buchet, Lucie Barthe, Liza Ammar‐Khodja, Bessam Azizi, Valentin Durante, Gianluca Cioci, Simon de Givry, Alain Roussel, Luis F. Garcia‐Alles, Thomas Schiex, Sophie Barbe   +12 more
wiley   +1 more source