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Nanobodies that Neutralize HIV [PDF]
Vaccines, 2019 Nanobodies or VHH (variable domains of heavy-chain only antibodies) are derived from camelid species such as llamas and camels. Nanobodies isolated and selected through phage display can neutralize a broad range of human immunodeficiency virus type 1 ...
Robin A. Weiss, C. Theo Verrips
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NANOBODIES: STRUCTURE, MANUFACTURING, APPLICATION (REVIEW)
Разработка и регистрация лекарственных средств, 2019 Introduction. Single-domain antibodies (nanobodies) are composed of the heavy-chain variable domain only. Compared to conventional immunoglobulins G (IgG) nanobodies have such qualities as: high bioavailability, ability to bind epitopes that are ...
A. V. Shatalova +3 more
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Oligoclonal selection of nanobodies targeting vascular endothelial growth factor
Journal of Immunotoxicology, 2019 While monoclonal antibodies are efficient therapeutics for cancer treatment, nanobodies or variable heavy domain – due to their small size, high stability, and solubility – have many advantages in comparison.
Mehrdad Ahadi +3 more
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Domain swapping of complementarity-determining region in nanobodies produced by Pichia pastoris [PDF]
AMB Express, 2019 Easy preparation of chimeric nanobodies with various scaffolds is important for customizing abilities of nanobodies toward practical utilization. To accomplish high-throughput production of various nanobodies, utilization of microbes is an attractive ...
Natsuko Miura +7 more
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Scientific Reports, 2021 Enterotoxigenic Escherichia coli (ETEC) is estimated to cause approximately 380,000 deaths annually during sporadic or epidemic outbreaks worldwide.
Alla Amcheslavsky +18 more
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Facile isolation of high-affinity nanobodies from synthetic libraries using CDR-swapping mutagenesis
STAR Protocols, 2022 Summary: The generation of high-affinity nanobodies for diverse biomedical applications typically requires immunization or affinity maturation. Here, we report a simple protocol using complementarity-determining region (CDR)-swapping mutagenesis to ...
Jennifer M. Zupancic +2 more
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Recent advances of nanobody applications in diagnosis and detection
MedComm – Biomaterials and Applications, 2023 Nanobodies (Nbs) are the variable domain of heavy‐chain antibodies derived from the blood of camelids or sharks. Nanobodies are the smallest antibody fragment with intact antigen‐binding ability.
Qianling Su +5 more
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A general approach for stabilizing nanobodies for intracellular expression
eLife, 2022 Conventional antibodies and their derived fragments are difficult to deploy against intracellular targets in live cells, due to their bulk and structural complexity.
John G Dingus +4 more
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Biparatopic nanobodies targeting the receptor binding domain efficiently neutralize SARS-CoV-2
iScience, 2022 Summary: The development of therapeutics to prevent or treat COVID-19 remains an area of intense focus. Protein biologics, including monoclonal antibodies and nanobodies that neutralize virus, have potential for the treatment of active disease.
Phillip Pymm +23 more
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Revolutionizing antiviral therapy with nanobodies: Generation and prospects
Biotechnology Reports, 2023 As the world continues to grapple with infectious diseases, scientists are constantly searching for effective ways to combat these deadly pathogens. One promising avenue of research is the use of nanobodies as neutralization agents. These small proteins,
Mujahed I. Mustafa, Ahmed Mohammed
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