Results 11 to 20 of about 11,503 (243)
Nanobodies that Neutralize HIV [PDF]
Vaccines, 2019 Nanobodies or VHH (variable domains of heavy-chain only antibodies) are derived from camelid species such as llamas and camels. Nanobodies isolated and selected through phage display can neutralize a broad range of human immunodeficiency virus type 1 ...
Robin A. Weiss, C. Theo Verrips
doaj +3 more sources
Structure of Nanobody Nb23 [PDF]
Molecules, 2021 Background: Nanobodies, or VHHs, are derived from heavy chain-only antibodies (hcAbs) found in camelids. They overcome some of the inherent limitations of monoclonal antibodies (mAbs) and derivatives thereof, due to their smaller molecular size and higher stability, and thus present an alternative to mAbs for therapeutic use.
Percipalle M. +4 more
openaire +5 more sources
Chemogenetic Control of Nanobodies [PDF]
Nature Methods, 2019 ABSTRACT We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows to study their role in basic biological processes, an approach that should be applicable to ...
Farrants, Helen +9 more
openaire +3 more sources
Immunogenicity Risk Profile of Nanobodies [PDF]
Frontiers in Immunology, 2021 Nanobodies (Nbs), the variable domains of camelid heavy chain-only antibodies, are a promising class of therapeutics or in vivo imaging reagents entering the clinic. They possess unique characteristics, including a minimal size, providing fast pharmacokinetics, high-target specificity, and an affinity in the (sub-)nanomolar range in conjunction with an
Chloé Ackaert +17 more
openaire +5 more sources
Antibodies, 2020 Since their first description in 1993 [...]
Patrick Chames, Ulrich Rothbauer
openaire +3 more sources
Domain swapping of complementarity-determining region in nanobodies produced by Pichia pastoris
AMB Express, 2019 Easy preparation of chimeric nanobodies with various scaffolds is important for customizing abilities of nanobodies toward practical utilization. To accomplish high-throughput production of various nanobodies, utilization of microbes is an attractive ...
Natsuko Miura +7 more
doaj +1 more source
Facile isolation of high-affinity nanobodies from synthetic libraries using CDR-swapping mutagenesis
STAR Protocols, 2022 Summary: The generation of high-affinity nanobodies for diverse biomedical applications typically requires immunization or affinity maturation. Here, we report a simple protocol using complementarity-determining region (CDR)-swapping mutagenesis to ...
Jennifer M. Zupancic +2 more
doaj +1 more source
Recombinant expression of nanobodies and nanobody-derived immunoreagents
Protein Expression and Purification, 2020 Antibody fragments for which the sequence is available are suitable for straightforward engineering and expression in both eukaryotic and prokaryotic systems. When produced as fusions with convenient tags, they become reagents which pair their selective binding capacity to an orthogonal function.
openaire +4 more sources
Nanobody-Targeted Photodynamic Therapy: Nanobody Production and Purification
, 2022 Nanobodies have recently been introduced to the field of photodynamic therapy (PDT) as a very promising strategy to target photosensitizers selectively to cancer cells. Nanobodies are known for their characteristic small size (15 kDa), high specificity, and high binding affinities.
Mashayekhi, Vida +4 more
openaire +4 more sources
Scientific Reports, 2021 Enterotoxigenic Escherichia coli (ETEC) is estimated to cause approximately 380,000 deaths annually during sporadic or epidemic outbreaks worldwide.
Alla Amcheslavsky +18 more
doaj +1 more source