Results 21 to 30 of about 16,217 (195)

Cell-Based Immunization Combined with Single-Round Cell Panning Enables Discovery of PSMA-Targeting Nanobodies from Phage Display Libraries

Biomolecules
There is a strong need for nanobodies that target novel cancer-associated antigens to advance radioligand imaging and antibody-based therapeutics. In this study, we investigated whether non-targeted llama immunization using tumor cells, combined with non-
Tong Yang, Joke Veldhoven-Zweistra, Maarten Ligtenberg, Sigrun Erkens, Mirella Vredenbregt-van den Berg, Rick Jansen, Patrick Chames, Eric M. J. Bindels, Khadijeh Ahmadi, Chris H. Bangma, Anton M. F. Kalsbeek, Janne Leivo, Nicolaas Lumen, Harmen J. G. van de Werken, Wytske M. van Weerden, Soudabeh Kavousipour, Raheleh Tooyserkani, Guido Jenster   +17 more
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Structure of Nanobody Nb23 [PDF]

Molecules, 2021
Background: Nanobodies, or VHHs, are derived from heavy chain-only antibodies (hcAbs) found in camelids. They overcome some of the inherent limitations of monoclonal antibodies (mAbs) and derivatives thereof, due to their smaller molecular size and higher stability, and thus present an alternative to mAbs for therapeutic use.
Percipalle M., Hunashal Y., Steyaert J., Fogolari F., Esposito G.   +4 more
openaire   +5 more sources

Nanobodies: From Discovery to AI-Driven Design

Biology
Nanobodies, derived from naturally occurring heavy-chain antibodies in camelids (VHHs) and sharks (VNARs), are unique single-domain antibodies that have garnered significant attention in therapeutic, diagnostic, and biotechnological applications due to ...
Haoran Zhu, Yu Ding
doaj   +2 more sources

Construction of an alpaca immune antibody library for the selection of nanobodies against Drosophila melanogaster proteins

Frontiers in Bioengineering and Biotechnology, 2023
Introduction:Drosophila melanogaster is a model organism for studying developmental biology and human neural disorders. Nanobodies are the variable domains of the heavy chains of camelid heavy-chain antibodies (VHHs) with high affinity to their antigens ...
Jianxiang Qiu, Jie Li, Zhen Zhang, Shirui Dong, Xiaomei Ling, Zhixin Fang, Quanshou Ling, Zhixin Huang   +7 more
doaj   +1 more source

Influence of nanobody binding on fluorescence emission, mobility, and organization of GFP-tagged proteins

iScience, 2021
Summary: Advanced fluorescence microscopy studies require specific and monovalent molecular labeling with bright and photostable fluorophores. This necessity led to the widespread use of fluorescently labeled nanobodies against commonly employed ...
Falk Schneider, Taras Sych, Christian Eggeling, Erdinc Sezgin   +3 more
doaj   +1 more source

Quantitative flow cytometric selection of tau conformational nanobodies specific for pathological aggregates

Frontiers in Immunology, 2023
Single-domain antibodies, also known as nanobodies, are broadly important for studying the structure and conformational states of several classes of proteins, including membrane proteins, enzymes, and amyloidogenic proteins.
Jennifer M. Zupancic, Jennifer M. Zupancic, Matthew D. Smith, Matthew D. Smith, Hanna Trzeciakiewicz, Mary E. Skinner, Sean P. Ferris, Emily K. Makowski, Emily K. Makowski, Michael J. Lucas, Michael J. Lucas, Michael J. Lucas, Nikki McArthur, Ravi S. Kane, Henry L. Paulson, Henry L. Paulson, Henry L. Paulson, Peter M. Tessier, Peter M. Tessier, Peter M. Tessier, Peter M. Tessier, Peter M. Tessier, Peter M. Tessier   +22 more
doaj   +1 more source

Chemogenetic Control of Nanobodies [PDF]

Nature Methods, 2019
ABSTRACT We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows to study their role in basic biological processes, an approach that should be applicable to ...
Farrants, Helen, Tarnawski, Miroslaw, Müller, Thorsten G., Otsuka, Shotaro, Hiblot, Julien, Koch, Birgit, Kueblbeck, Moritz, Kräusslich, Hans-Georg, Ellenberg, Jan, Johnsson, Kai   +9 more
openaire   +4 more sources

A Small Virus to Deliver Small Antibodies: New Targeted Therapies Based on AAV Delivery of Nanobodies

Microorganisms, 2021
Nanobodies are camelid-derived single-domain antibodies that present some advantages versus conventional antibodies, such as a smaller size, and higher tissue penetrability, stability, and hydrophilicity. Although nanobodies can be delivered as proteins,
Noelia Silva-Pilipich, Cristian Smerdou, Lucía Vanrell   +2 more
doaj   +1 more source

Nanobody: a promising toolkit for molecular imaging and disease therapy

EJNMMI Research, 2021
Nanobodies are the recombinant variable domains of heavy-chain-only antibodies, with many unique properties such as small size, excellent solubility, superior stability, quick clearance from blood, and deep tissue penetration.
Guangfa Bao, Ming Tang, Jun Zhao, Xiaohua Zhu   +3 more
doaj   +1 more source

Special Issue: Nanobody [PDF]

Antibodies, 2020
Since their first description in 1993 [...]
Patrick Chames, Ulrich Rothbauer
openaire   +3 more sources