Domain swapping of complementarity-determining region in nanobodies produced by Pichia pastoris
AMB Express, 2019 Easy preparation of chimeric nanobodies with various scaffolds is important for customizing abilities of nanobodies toward practical utilization. To accomplish high-throughput production of various nanobodies, utilization of microbes is an attractive ...
Natsuko Miura +7 more
doaj +1 more source
Facile isolation of high-affinity nanobodies from synthetic libraries using CDR-swapping mutagenesis
STAR Protocols, 2022 Summary: The generation of high-affinity nanobodies for diverse biomedical applications typically requires immunization or affinity maturation. Here, we report a simple protocol using complementarity-determining region (CDR)-swapping mutagenesis to ...
Jennifer M. Zupancic +2 more
doaj +1 more source
A new survivin tracer tracks, delocalizes and captures endogenous survivin at different subcellular locations and in distinct organelles [PDF]
, 2016 Survivin, the smallest member of the inhibitor of apoptosis protein family, plays a central role during mitosis and exerts a cytoprotective function. Survivin is highly expressed in most cancer types and contributes to multiple facets of carcinogenesis ...
Beghein, Els +5 more
core +2 more sources
Scientific Reports, 2021 Enterotoxigenic Escherichia coli (ETEC) is estimated to cause approximately 380,000 deaths annually during sporadic or epidemic outbreaks worldwide.
Alla Amcheslavsky +18 more
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Identification and characterization of nanobodies targeting the EphA4 receptor [PDF]
, 2017 The ephrin receptor A4 (EphA4) is one of the receptors in the ephrin system that plays a pivotal role in a variety of cell-cell interactions, mostly studied during development. In addition, EphA4 has been found to play a role in cancer biology as well as
Chavez-Gutierrez, Lucia +16 more
core +2 more sources
Chemogenetic Control of Nanobodies [PDF]
Nature Methods, 2019 ABSTRACTWe introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows to study their role in basic biological processes, an approach that should be applicable to numerous previously ...
Farrants, Helen +9 more
openaire +3 more sources
Recent advances of nanobody applications in diagnosis and detection
MedComm – Biomaterials and Applications, 2023 Nanobodies (Nbs) are the variable domain of heavy‐chain antibodies derived from the blood of camelids or sharks. Nanobodies are the smallest antibody fragment with intact antigen‐binding ability.
Qianling Su +5 more
doaj +1 more source
Selective engagement of FcγRIV by a M2e-specific single domain antibody construct protects against influenza A virus infection [PDF]
, 2019 Lower respiratory tract infections, such as infections caused by influenza A viruses, are a constant threat for public health. Antivirals are indispensable to control disease caused by epidemic as well as pandemic influenza A.
Ballegeer, Marlies +10 more
core +2 more sources
NANOBODIES: STRUCTURE, MANUFACTURING, APPLICATION (REVIEW)
Разработка и регистрация лекарственных средств, 2019 Introduction. Single-domain antibodies (nanobodies) are composed of the heavy-chain variable domain only. Compared to conventional immunoglobulins G (IgG) nanobodies have such qualities as: high bioavailability, ability to bind epitopes that are ...
A. V. Shatalova +3 more
doaj +1 more source
A general approach for stabilizing nanobodies for intracellular expression
eLife, 2022 Conventional antibodies and their derived fragments are difficult to deploy against intracellular targets in live cells, due to their bulk and structural complexity.
John G Dingus +4 more
doaj +1 more source