Results 151 to 160 of about 18,503 (241)

Self-Blinking Dye Restores Efficient Use of Nanobodies in Single-Molecule Localization Microscopy. [PDF]

Nano Lett
Basak S, Inamdar K, Pollack YG, Albert L, Jans DC, Jakobs S, Enderlein J, Tsukanov R, Opazo F.   +8 more
europepmc   +1 more source

Ligand binding represses bacterial histidine kinase activity by inhibiting its dimerization

The FEBS Journal, EarlyView.
PdtaS is a kinase in the pathogen Mycobacterium tuberculosis that senses copper and nitric oxide and controls virulence gene expression, but how the kinase senses these compounds was unclear. We show that PdtaS is active as a dimer with each monomer phosphorylating the other in trans.
Gaurav D. Sankhe, Jiawei Xing, Xiansha Xiao, John A. Buglino, Huilin Li, Igor B. Zhulin, Michael S. Glickman   +6 more
wiley   +1 more source

Targeting angiogenesis: advances in the design and engineered applications of nanobodies. [PDF]

Front Immunol
Liu Y, Li Z, Ma Y, Liu Y.
europepmc   +1 more source

Preclinical Target Animal Safety Evaluation of Veterinary Monoclonal Antibodies: Principles, Methods, and Interpretation

Journal of Veterinary Pharmacology and Therapeutics, EarlyView.
ABSTRACT Monoclonal antibodies (mAbs) represent a rapidly expanding pharmaceutical class in veterinary medicine, with several products now authorized for use in dogs and cats. Limited published articles and veterinarian exposure to the principles of drug development present challenges in interpreting published safety evaluations that inform product ...
Adam Werts, Matthew Kuhn, Lauren Bessey, Alicia Braxton Hickman, Emily Chory, John Mitchell, Kirby Pasloske   +6 more
wiley   +1 more source

A neuraminidase-targeting nanobody as a therapeutic candidate against influenza A and B viruses. [PDF]

J Virol
Liu D, Zhang Y, Zhang M, Guo R, Peng W, Cao R, Yang X, Chen J, Huang S, Li J, Sheng C, Qin L.   +11 more
europepmc   +1 more source

TurboID‐based proteomic profiling reveals proxitome of the IRT1 metal transporter and new insight into IRT1 endocytosis

New Phytologist, EarlyView.
Identification of new regulators of Arabidopsis IRT1 endocytosis and metal transport using TurboID. TurboID proximity labeling of the Arabidopsis IRT1 root metal transporter identified new IRT1‐interacting proteins upon different metal regimes. Among validated candidates, the endosome‐localized NHX5 Na+ (K+)/H+ antiporter and the plasma membrane ...
Liza Pellegrin, Steven Fanara, Bertrand Fabre, Carole Pichereaux, Nathalie Leonhardt, Valérie Cotelle, Grégory Vert, Julie Neveu   +7 more
wiley   +1 more source

Bacteriophage P22 Virus‐Like Particles as Nanoscale Protein Scaffolds for Plant Synthetic Biology

Plant Biotechnology Journal, EarlyView.
ABSTRACT Advancing the utility of plant synthetic biology requires the continued development of protein engineering tools. Self‐assembling protein compartments, such as virus‐like particles (VLPs), provide versatile scaffolds for synthetic biology. However, few plant‐expressed VLPs have demonstrated broad amenability to protein engineering, restricting
Maxim D. Harding, Mark A. Jackson, Edward K. Gilding, David J. Craik, Frank Sainsbury, Nicole Lawrence   +5 more
wiley   +1 more source

Efficient discovery of an agonistic anti-OX40 nanobody by epitope-directed approach to address enrichment-driven epitope bias. [PDF]

J Immunother Cancer
Iemura T, Kitawaki T, Maeda R, Mitsuyoshi T, Nagata K, Yamazaki H, Shirakawa K, Kobayashi N, Nagata T, Imura A, Takaori-Kondo A.   +10 more
europepmc   +1 more source

Programmable Domestication: CRISPR, Pan‐Genomics and System Level Engineering for Next‐Generation Crops

Plant Biotechnology Journal, EarlyView.
Conceptual overview of programmable de novo domestication from genetic diversity discovery to crop deployment. The framework connects diverse germplasm and genomic resources with three trajectory pathways. Adaptive Rescue, Agronomic Refinement and Novel Chassis defined by biological distance, engineering complexity and breeding objectives.
Muhammad Mubashar Zafar, Hina Firdous, Ayesha Siddiqua, Ayesha Naveed, Abdul Razzaq, Sadam Munawar, Aqsa Ijaz, Zunaira Anwar, Sezai Ercisli, Xuefei Jiang, Qiao Fei   +10 more
wiley   +1 more source

A tool to pulse-label yeast nuclear pore complexes in imaging and biochemical experiments. [PDF]

Elife
Veldsink AC, Fischer JS, Hell S, Weis K, Veenhoff LM.   +4 more
europepmc   +1 more source