Results 171 to 180 of about 5,929 (191)
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Biochemical Society Transactions, 2020
The small engineered luciferase NanoLuc has rapidly become a powerful tool in the fields of biochemistry, chemical biology, and cell biology due to its exceptional brightness and stability. The continuously expanding NanoLuc toolbox has been employed in applications ranging from biosensors to molecular and cellular imaging, and currently includes split
Biewenga, Lieuwe +2 more
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The small engineered luciferase NanoLuc has rapidly become a powerful tool in the fields of biochemistry, chemical biology, and cell biology due to its exceptional brightness and stability. The continuously expanding NanoLuc toolbox has been employed in applications ranging from biosensors to molecular and cellular imaging, and currently includes split
Biewenga, Lieuwe +2 more
openaire +1 more source
Aryliden-imidazolones as fluorogens of NanoLuc protein
Биоорганическая химияWe report a series of orto-substituted aryliden-imidazolones and their derivatives containing styrene moiety. These compounds can be used like ligands of NanoLuc protein. Together with NanoLuc this fluorogens can be used for genetically encoded labeling in fluorescence microscopy, as demonstrated by staining HEK293 cells.
E. R. Zaitseva +4 more
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Generation of recombinant rotaviruses encoding a split NanoLuc peptide tag
Biochemical and Biophysical Research Communications, 2021Recombinant viruses expressing fluorescent or luminescent reporter proteins are used to quantitate and visualize viral replication and transmission. Here, we used a split NanoLuc luciferase (NLuc) system comprising large LgBiT and small HiBiT peptide fragments to generate stable reporter rotaviruses (RVs).
Pimfhun Pannacha +8 more
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SOD1 dimerization monitoring using a novel split NanoLuc, NanoBit
Cell Biochemistry and Function, 2016In the present study, we applied a highly sensitive NanoLuc‐based technology to understand the status of superoxide dismutase 1 (SOD1) within mammalian cells. Two fragments of NanoLuc (NanoBit), large N‐terminal and small C‐terminal regions, were fused with wild‐type (wt) and mutant human SOD1 (hSOD1) genes and transfected into cells.
Kentaro, Oh-Hashi +2 more
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Behind the glow: unveiling the nature of NanoLuc reactants and products
Physical Chemistry Chemical PhysicsAmong all the possible candidates involved in the NanoLuc bioluminiescnce, this computational analysis identifies the enol tautomer of furimazine as a probable reactive species and the zwitterionic forms of furimamide as the luminescent product.
Alessandro Bonardi +5 more
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A sensitive assay for the biosynthesis and secretion of MANF using NanoLuc activity
Biochemical and Biophysical Research Communications, 2014Mesencephalic astrocyte-derived neurotrophic factor (MANF) has been reported to prevent neuronal cell death caused by certain stimuli. Accordingly, the molecular features of MANF have been intensively investigated since the reporting of its cytoprotective actions.
Junpei, Norisada +4 more
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Dual Luciferase Assay for Secreted Luciferases Based on Gaussia and NanoLuc
ASSAY and Drug Development Technologies, 2013Just recently, NanoLuc, a new engineered luciferase based on the small subunit of the luciferase from Oplophorus gracilirostris was introduced. Like the luciferase from Gaussia princeps, this luciferase is secreted into the medium. Both luciferases are the smallest and brightest luciferases known and well-suited for reporter assays. In our experiments,
Kerstin, Heise +4 more
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Evaluation of NanoLuc substrates for bioluminescence imaging of transferred cells in mice
Journal of Photochemistry and Photobiology B: Biology, 2021Joel R Walker +2 more
exaly
Mechanical Stability of a Small, Highly-Luminescent Engineered Protein NanoLuc
International Journal of Molecular Sciences, 2021Yue Ding +2 more
exaly