Results 171 to 180 of about 6,462 (191)

Highly Potent Cell-Permeable and Impermeable NanoLuc Luciferase Inhibitors

ACS Chemical Biology, 2017
Novel engineered NanoLuc (Nluc) luciferase being smaller, brighter, and superior to traditional firefly (Fluc) or Renilla (Rluc) provides a great opportunity for the development of numerous biological, biomedical, clinical, and food and environmental safety applications.
Joel R. Walker, Mary P. Hall, Chad A. Zimprich, Matthew B. Robers, Sarah J. Duellman, Thomas Machleidt, Jacquelynn Rodriguez, Wenhui Zhou   +7 more
openaire   +2 more sources

SOD1 dimerization monitoring using a novel split NanoLuc, NanoBit

Cell Biochemistry and Function, 2016
In the present study, we applied a highly sensitive NanoLuc‐based technology to understand the status of superoxide dismutase 1 (SOD1) within mammalian cells. Two fragments of NanoLuc (NanoBit), large N‐terminal and small C‐terminal regions, were fused with wild‐type (wt) and mutant human SOD1 (hSOD1) genes and transfected into cells.
Kentaro, Oh-Hashi, Yoko, Hirata, Kazutoshi, Kiuchi   +2 more
openaire   +2 more sources

Behind the glow: unveiling the nature of NanoLuc reactants and products

Physical Chemistry Chemical Physics
Among all the possible candidates involved in the NanoLuc bioluminiescnce, this computational analysis identifies the enol tautomer of furimazine as a probable reactive species and the zwitterionic forms of furimamide as the luminescent product.
Alessandro Bonardi, Michele Turelli, Giorgio Moro, Claudio Greco, Ugo Cosentino, Carlo Adamo   +5 more
openaire   +3 more sources

A sensitive assay for the biosynthesis and secretion of MANF using NanoLuc activity

Biochemical and Biophysical Research Communications, 2014
Mesencephalic astrocyte-derived neurotrophic factor (MANF) has been reported to prevent neuronal cell death caused by certain stimuli. Accordingly, the molecular features of MANF have been intensively investigated since the reporting of its cytoprotective actions.
Junpei, Norisada, Yoko, Hirata, Fumimasa, Amaya, Kazutoshi, Kiuchi, Kentaro, Oh-hashi   +4 more
openaire   +2 more sources

Bright and Orthogonal NanoLuc Substrates

Synfacts, 2020
openaire   +1 more source

Mechanical Stability of a Small, Highly-Luminescent Engineered Protein NanoLuc

International Journal of Molecular Sciences, 2021
Piotr Marszalek, Yue Ding, Dimitra Apostolidou   +2 more
exaly  

Apaf1 nanoLuc biosensors identified lentinan as a potent synergizer of cisplatin in targeting hepatocellular carcinoma cells

Biochemical and Biophysical Research Communications, 2021
Haining Fan, Zhixin Wang, Li Ren
exaly  

Nanoluc suicide substrates

2023
BINKOWSKI BROCK, HALL MARY P, MACHLEIDT THOMAS, WALKER JOEL, ZHOU WENHUI   +4 more
openaire   +1 more source

Structural basis for NanoLuc luciferase action

Acta Crystallographica Section A Foundations and Advances
Martin Marek, M. Nemergut, D. Pluskal, J. Horackova, T. Sustrova, T. Barta, M. Toul, Z. Prokop, D. Bednar, Y. L. Janin, J. Damborsky   +10 more
openaire   +1 more source

Comparative Analysis of NanoLuc Luciferase and Alkaline Phosphatase Luminescence Reporter Systems for Phage-Based Detection of Bacteria

Bioengineering, 2022
Joey N Talbert
exaly