Results 161 to 170 of about 6,462 (191)
A Split NanoLuc Reporter Quantitatively Measures Circular RNA IRES Translation [PDF]
Genes, 2022 Internal ribosomal entry sites (IRESs) are RNA secondary structures that mediate translation independent from the m7G RNA cap. The dicistronic luciferase assay is the most frequently used method to measure IRES-mediated translation.
Shu Jun Lin +2 more
exaly +2 more sources
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Caspase sensors based on NanoLuc
Journal of Biotechnology, 2022Caspases are a family of evolutionary conserved cysteine proteases that play key roles in programmed cell death and inflammation. Among the methods for the detection of caspase activity, biosensors based on luciferases have advantages in genetical encoding and convenience in assay.
Chun-Yang Gan, Xue-Fei Cai
exaly +3 more sources
Evaluation of NanoLuc, RedLuc and Luc2 as bioluminescent reporters in a cutaneous leishmaniasis model [PDF]
Acta Tropica, 2020 New drugs for the treatment of human leishmaniasis are urgently needed, considering the limitations of current available options. However, pre-clinical evaluation of drug candidates for leishmaniasis is challenging.
Silvia R B Uliana, Helen P Price
exaly +2 more sources
NanoLuc Luciferase as a Fluorogen-Activating Protein for GFP Chromophore Based Fluorogens
International Journal of Molecular Sciences, 2023 In this work, we showed that the well-known NanoLuc luciferase can act as a fluorogen activating protein for various arylidene-imidazolones structurally similar to the Kaede protein chromophore.
Sergey Goncharuk +2 more
exaly +2 more sources
A NanoLuc-Based Transactivation Assay in Plants
2023Protein-DNA interactions are determinant of the regulation of gene expression in living organisms. Luminescence studies have been used in a wide range of techniques to identify how gene transcription can be regulated by proteins such as transcription factors (TFs).
Rosa Esmeralda, Becerra-García +4 more
openaire +2 more sources
Biochemical Society Transactions, 2020
The small engineered luciferase NanoLuc has rapidly become a powerful tool in the fields of biochemistry, chemical biology, and cell biology due to its exceptional brightness and stability. The continuously expanding NanoLuc toolbox has been employed in applications ranging from biosensors to molecular and cellular imaging, and currently includes split
Biewenga, Lieuwe +2 more
openaire +1 more source
The small engineered luciferase NanoLuc has rapidly become a powerful tool in the fields of biochemistry, chemical biology, and cell biology due to its exceptional brightness and stability. The continuously expanding NanoLuc toolbox has been employed in applications ranging from biosensors to molecular and cellular imaging, and currently includes split
Biewenga, Lieuwe +2 more
openaire +1 more source
Novel NanoLuc-type substrates with various C-6 substitutions
Bioorganic and Medicinal Chemistry Letters, 2020NanoLuc (NLuc)-furimazine bioluminescence system offers several advantages over established systems, including improved stability, smaller size, and >150-fold enhancement in bioluminescence. Herein, we designed and synthesized a series of bioluminescent substrates with varying at the C-6 position of furimazine for NLuc-furimazine bioluminescence system.
Lupei Du, Minyong Li
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Rapid titration of recombinant baculoviruses based on NanoLuc secretion in early infection
Journal of Virological Methods, 2022The baculovirus expression vector system has become a powerful tool for recombinant protein production and gene delivery. However, existing titration methods for baculovirus are not economical in terms of test time and cost. A titration method based on NanoLuc secretion that allows for titration of recombinant baculoviruses at 4 h post infection (hpi ...
Jianqing Chen
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Aryliden-imidazolones as fluorogens of NanoLuc protein
Биоорганическая химияWe report a series of orto-substituted aryliden-imidazolones and their derivatives containing styrene moiety. These compounds can be used like ligands of NanoLuc protein. Together with NanoLuc this fluorogens can be used for genetically encoded labeling in fluorescence microscopy, as demonstrated by staining HEK293 cells.
E. R. Zaitseva +4 more
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Generation of recombinant rotaviruses encoding a split NanoLuc peptide tag
Biochemical and Biophysical Research Communications, 2021Recombinant viruses expressing fluorescent or luminescent reporter proteins are used to quantitate and visualize viral replication and transmission. Here, we used a split NanoLuc luciferase (NLuc) system comprising large LgBiT and small HiBiT peptide fragments to generate stable reporter rotaviruses (RVs).
Pimfhun Pannacha +8 more
openaire +2 more sources