Results 81 to 90 of about 998,588 (389)

DNA Sequencing by Hexagonal Boron Nitride Nanopore: A Computational Study

Nanomaterials, 2016
The single molecule detection associated with DNA sequencing has motivated intensive efforts to identify single DNA bases. However, little research has been reported utilizing single-layer hexagonal boron nitride (hBN) for DNA sequencing.
Liuyang Zhang, Xianqiao Wang
doaj   +1 more source

cgMSI: pathogen detection within species from nanopore metagenomic sequencing data

BMC Bioinformatics, 2023
Background Metagenomic sequencing is an unbiased approach that can potentially detect all the known and unidentified strains in pathogen detection. Recently, nanopore sequencing has been emerging as a highly potential tool for rapid pathogen detection ...
Xu Zhu, Lili Zhao, Lihong Huang, Wenxian Yang, Liansheng Wang, Rongshan Yu   +5 more
doaj   +1 more source

Comprehensive genetic diagnosis of tandem repeat expansion disorders with programmable targeted nanopore sequencing

medRxiv, 2021
Short-tandem repeat (STR) expansions are an important class of pathogenic genetic variants. Over forty neurological and neuromuscular diseases are caused by STR expansions, with 37 different genes implicated to date.
Igor Stevanovski, Sanjog R. Chintalaphani, Hasindu Gamaarachchi, James M. Ferguson, S. S. Pineda, C. Scriba, Michel C. Tchan, Victor S. C. Fung, K. Ng, A. Cortese, H. Houlden, C. Dobson-Stone, L. Fitzpatrick, G. Halliday, G. Ravenscroft, M. Davis, N. Laing, Avi Fellner, M. Kennerson, K. Kumar, I. Deveson   +20 more
semanticscholar   +1 more source

Laser‐Based Sculpturing of Embedded Ultrathin Metal‐Oxide Nanopores for Enhanced Biomolecular Sensing

Advanced Functional Materials, EarlyView.
Controlled laser‐drilling of embedded HfO2 membranes creates three layer nanopores with Gaussian‐shaped cavities sculptured in the supporting layers. These embedded solid‐state nanopores slow DNA translocation by 12‐fold compared to SiNx pores, enabling high‐resolution, label‐free detection of short DNAs, RNAs, and proteins.
Jostine Joby, Yulia Marom, Guocong Lin, Aleksandra Baron‐Wiechec, Amit Meller   +4 more
wiley   +1 more source

Nanopore sequencing for smear-negative pulmonary tuberculosis—a multicentre prospective study in China

Annals of Clinical Microbiology and Antimicrobials
Purpose In this prospective study, the diagnosis accuracy of nanopore sequencing-based Mycobacterium tuberculosis (MTB) detection was determined through examining bronchoalveolar lavage fluid (BALF) samples from pulmonary tuberculosis (PTB) -suspected ...
Xiaojing Yan, Guoli Yang, Yunfei Wang, Yuqing Wang, Jie Cheng, Peisong Xu, Xiaoli Qiu, Lei Su, Lina Liu, Ruixue Geng, Yingxia You, Hui Liu, Naihui Chu, Li Ma, Wenjuan Nie   +14 more
doaj   +1 more source

Nanopore sequencing of single-cell transcriptomes with scCOLOR-seq

Nature Biotechnology, 2021
Here we describe single-cell corrected long-read sequencing (scCOLOR-seq), which enables error correction of barcode and unique molecular identifier oligonucleotide sequences and permits standalone cDNA nanopore sequencing of single cells.
M. Philpott, J. Watson, A. Thakurta, T. Brown, T. Brown, U. Oppermann, Adam P. Cribbs   +6 more
semanticscholar   +1 more source

Electron–Matter Interactions During Electron Beam Nanopatterning

Advanced Functional Materials, EarlyView.
This article reviews the electron–matter interactions important to nanopatterning with electron beam lithography (EBL). Electron–matter interactions, including secondary electron generation routes, polymer radiolysis, and electron beam induced charging, are discussed.
Camila Faccini de Lima, Prathiba Thamizhavel, Taylor J. Woehl   +2 more
wiley   +1 more source

NAD tagSeq reveals that NAD+-capped RNAs are mostly produced from a large number of protein-coding genes in Arabidopsis. [PDF]

, 2019
The 5' end of a eukaryotic mRNA transcript generally has a 7-methylguanosine (m7G) cap that protects mRNA from degradation and mediates almost all other aspects of gene expression.
Cai, Zongwei, Chen, Xuemei, Ni, Min, Shao, Xiaojian, Xia, Yiji, Zhang, Hailei, Zhang, Shoudong, Zhong, Huan   +7 more
core  

Robust methylation‐based classification of brain tumours using nanopore sequencing

Neuropathology and Applied Neurobiology, 2022
DNA methylation‐based classification of cancer provides a comprehensive molecular approach to diagnose tumours. In fact, DNA methylation profiling of human brain tumours already profoundly impacts clinical neuro‐oncology.
L. P. Kuschel, J. Hench, Stephan Frank, Ivana Bratic Hench, E. Girard, Maud Blanluet, J. Masliah-Planchon, M. Misch, J. Onken, Marcus Czabanka, Dongsheng Yuan, Sören Lukassen, Philipp Karau, N. Ishaque, E. Hain, F. Heppner, A. Idbaih, Nikolaus Behr, Christoph Harms, D. Capper, P. Euskirchen   +20 more
semanticscholar   +1 more source

Nanopore Sequencing: Forcing Improved Resolution [PDF]

Biophysical Journal, 2015
In 2012, nanopore DNA sequencing became a reality, with the publication of a proof-of-concept by the academic team led by Akeson and Gundlach (1) and the nearly simultaneous announcement (without publication) by Oxford Nanopore Technologies (Oxford Science Park, Oxford, UK) that the company had successfully sequenced DNA strands measuring tens of ...
openaire   +2 more sources