Results 91 to 100 of about 63,196 (293)

Telomere‐to‐Telomere Genomes Reveal that Multiscale Evolution Shapes the Largest Metabolic Arsenal of Diaporthe Fungi

Advanced Science, EarlyView.
This study presents the first telomere‐to‐telomere genomes and population resources for Diaporthe pathogens, uncovering the largest known fungal repertoire of secondary metabolite gene clusters. Structural variations and horizontal gene transfer drive cluster diversification, while specific rapidly evolving clusters control virulence, offering novel ...
Kainan Li, Chen Zhang, Zhichao Zhang, Jiaheng Cheng, Qiuyan Gao, Long Gao, Xiaolin Zhao, Wei Yan, Yuanchao Wang, Wenwu Ye   +9 more
wiley   +1 more source

Diagnosis of pathogens causing bacterial meningitis using Nanopore sequencing in a resource-limited setting

Annals of Clinical Microbiology and Antimicrobials, 2022
Aim The aim of the present study is to compare the performance of 16S rRNA Nanopore sequencing and conventional culture in detecting infectious pathogens in patients with suspected meningitis in a resource-limited setting without extensive bioinformatics
Srinivas Reddy Pallerla, Do Van Dong, Le Thi Kieu Linh, Trinh Van Son, Dao Thanh Quyen, Phan Quoc Hoan, Ngo Tat Trung, Nguyen Trong The, Jule Rüter, Sébastien Boutin, Dennis Nurjadi, Bui Tien Sy, Peter G. Kremsner, Christian G. Meyer, Le Huu Song, Thirumalaisamy P. Velavan   +15 more
doaj   +1 more source

Fundamental Bounds for Sequence Reconstruction From Nanopore Sequencers

IEEE Transactions on Molecular, Biological and Multi-Scale Communications, 2016
Nanopore sequencers are emerging as promising new platforms for high-throughput sequencing. As with other technologies, sequencer errors pose a major challenge for their effective use. In this paper, we present a novel information theoretic analysis of the impact of insertion-deletion (indel) errors in nanopore sequencers.
Abram Magner, Jaroslaw Duda, Wojciech Szpankowski, Ananth Grama   +3 more
openaire   +2 more sources

Assessing Graphene Nanopores for Sequencing DNA [PDF]

Nano Letters, 2012
Using all-atom molecular dynamics and atomic-resolution Brownian dynamics, we simulate the translocation of single-stranded DNA through graphene nanopores and characterize the ionic current blockades produced by DNA nucleotides. We find that transport of single DNA strands through graphene nanopores may occur in single nucleotide steps.
David B, Wells, Maxim, Belkin, Jeffrey, Comer, Aleksei, Aksimentiev   +3 more
openaire   +2 more sources

Endocytic Control of Cell‐Autonomous and Non‐Cell‐Autonomous Functions of p53

Advanced Science, EarlyView.
NUMB Ex3‐containing isoforms localize to the plasma membrane, where they recruit p53 through SNX9 and direct it to multivesicular bodies and exosomes. Exported p53 is taken up by neighboring cells and activates nuclear programs, revealing an intercellular, exosome‐based pathway that might help establish a tumor‐suppressive microenvironment.
Roberta Cacciatore, Andrea Basile, Stefano Freddi, Irene Schiano Lomoriello, Carlo Ribelle Zucca, Giuseppe Ciossani, Luigi Scietti, Alessandro Cuomo, Simona Ronzoni, Simone Pelicci, Mario Faretta, Elena Zaccheroni, Giuliana Pelicci, Vittoria Matafora, Angela Bachi, Rosalind Helen Gunby, Salvatore Pece, Sara Sigismund, Letizia Lanzetti, Ivan Nicola Colaluca, Pier Paolo Di Fiore   +20 more
wiley   +1 more source

An Artificial Antibody‐Based Toolbox Accelerates Validation of Hidden Microproteins Encoded by the Dark Genome

Advanced Science, EarlyView.
Microproteins are hidden treasures encoded by the “dark proteome” but remain largely underexplored due to the lack of highly efficient tools. We developed a molecularly imprinted polymers (MIPs)‐based toolbox (CLAIMID) to achieve accelerated and ultrasensitive microproteins validation at multiple biological scales (single living cells, cell populations,
Hui He, Anqi Zhang, Zhanchen Guo, Qi Li, Song Gao, Wenyan Guan, Ying Li, Jingran Chen, Sijia Sun, Jian He, Zhen Liu   +10 more
wiley   +1 more source

DNA nucleotide-specific modulation of \mu A transverse edge currents through a metallic graphene nanoribbon with a nanopore

, 2011
We propose two-terminal devices for DNA sequencing which consist of a metallic graphene nanoribbon with zigzag edges (ZGNR) and a nanopore in its interior through which the DNA molecule is translocated.
Areshkin D., Areshkin D. A., Brandbyge M., Branislav K. Nikolić, Brey L., Cai J., Cervantes-Sodi F., Fyta M., Garaj S., Geim A. K., Hasan M. Z., He Y., Healy K., Huang S., Jia X., Kamal K. Saha, Krems M., Kunstmann J., Lagerqvist J., Marija Drndić, Merchant C. A., Meunier V., Nelson T., Postma H. W. C., Prasongkit J., Rungger I., Rycerz A., Saha K. K., Saha K. K., Schadt E. E., Schneider G. F., Strange M., Tao C., Taylor J., Tsutsui M., Venkatesan B. M., Weeks C., Yazyev O. V., Zwolak M., Zârbo L. P.   +39 more
core   +1 more source

2D Copper MOF Membranes with Precise Pores for Ionic Memory

Advanced Science, EarlyView.
 . ABSTRACT Biological ion channels play a critical role in the complex physiological processes of living organisms. Inspired by these natural systems, we demonstrate the fabrication of ultrathin 2D copper‐based metal‐organic framework (CuMOF) films and their application to mimic key functionalities of biological membranes. Using a liquid–air interface
Hiran Jyothilal, Raj Kumar Gogoi, Yuchen Liu, Vikas Sharma, Solleti Goutham, Michael Hutton, Rachel C. Kilbride, Daniel T.W. Toolan, Ashok Keerthi, Boya Radha   +9 more
wiley   +1 more source

Improving nanopore read accuracy with the R2C2 method enables the sequencing of highly multiplexed full-length single-cell cDNA. [PDF]

, 2018
High-throughput short-read sequencing has revolutionized how transcriptomes are quantified and annotated. However, while Illumina short-read sequencers can be used to analyze entire transcriptomes down to the level of individual splicing events with ...
Byrne, Ashley, Cole, Charles, Green, Richard E, Palmer, Theron, Schmitz, Robert J, Volden, Roger, Vollmers, Christopher   +6 more
core   +1 more source

Models and information-theoretic bounds for nanopore sequencing

, 2018
Nanopore sequencing is an emerging new technology for sequencing DNA, which can read long fragments of DNA (~50,000 bases) in contrast to most current short-read sequencing technologies which can only read hundreds of bases. While nanopore sequencers can
Diggavi, Suhas, Kannan, Sreeram, Mao, Wei   +2 more
core   +1 more source