Results 81 to 90 of about 938,732 (287)

Nanopores: a sequencer in your backpack [PDF]

Nature Methods, 2015
It's been a long wait for nanopore sequencing technology. Over 1,000 labs are testing the first commercial device and publishing results. Researchers tell Nature Methods about their experiences putting these early instruments through their paces. And more technology is in the works.
openaire   +2 more sources

A Real‐Data‐Driven Framework for Evaluating Differential Transcript Usage Methods Across Long‐Read Bulk, Single‐Cell, and Spatial Transcriptomics

Advanced Science, EarlyView.
Grounded in real biological RNA‐seq data, the framework benchmarks ten differential transcript usage (DTU) methods across short‐read bulk, long‐read bulk, long‐read single‐cell, and long‐read spatial transcriptomics. By integrating reference transcript sets with ranked DTU results, the Transcript Set Enrichment Score (TSES) identifies data‐type ...
Chenxing Zhang, Jun Liu, Qi Zhao, Huilong Yin, Angang Yang, Minhua Zheng, Rui Zhang   +6 more
wiley   +1 more source

Is the Volume Exclusion Model Practicable for Nanopore Protein Sequencing?

, 2021
The nanopore approach holds the possibility for achieving single-molecule protein sequencing. However, ongoing challenges still remain in the biological nanopore technology, which aims to identify 20 natural amino acids by reading the ionic current ...
Ming-Zhu Huo, Yi-Tao Long (1409407), Ming-Zhu Huo (11262238), Yi-Lun Ying (1686139), Yi-Lun Ying, Meng-Yin Li, Yi-Tao Long, Meng-Yin Li (5367758)   +7 more
core   +1 more source

PTBP1‐Mediated Alternative Splicing of DNAJB6 Promotes Everolimus Resistance in Clear Cell Renal Cell Carcinoma via EIF4B/PKIB/AKT/mTOR Positive Feedback Loop

Advanced Science, EarlyView.
PTBP1‐mediated alternative splicing drives a pathogenic switch to the DNAJB6b isoform in everolimus‐resistant clear cell renal cell carcinoma. This splicing event programs a positive feedback loop including EIF4B/PKIB/AKT/mTOR, promoting aggressive therapy resistance.
Xiu‐wu Pan, Hong‐feng Zheng, Mu‐chen Li, Jia‐lin Zhou, Hang‐biao Zhang, Dong‐hao Lyu, Yi‐fan Tang, Tian‐yue Yang, Yi‐fan Liu, Zi‐chang Liu, Wen‐jie Ma, Yuan‐bo Zong, Jian‐gui Liu, Zi‐xuan Gong, Hao Zhang, Ke‐qin Dong, Wang Zhou, Jian‐qin Ye, Xin‐gang Cui   +18 more
wiley   +1 more source

Nanopore Protein Sequencing

Shengwu huaxue yu shengwu wuli jinzhan
The success of the Human Genome Project has significantly deepened our understanding of genomics and catalyzed a growing focus on proteomics, as researchers aim to decipher the complex relationship between genes and proteins.
LI Zi-Yi, YI Ya-Kun, LIU Lei, WU Hai-Chen   +3 more
doaj   +1 more source

Individual nanopore sequencing run characteristics.

Individual nanopore sequencing run characteristics.
Mohamed Ali Ali (17889541), Daniel J. Bruzzese (6807488), Safia Mohammed Aliy (17889535), Anita Lerch (2029), Joshua Yukich (44606), Aurel Holzschuh (8376513), Mohamed Haji Ali (17889538), Manuel W. Hetzel (9773118), Bakar S. Fakih (17889532), Cristian Koepfli (191306)   +9 more
core   +1 more source

Expanding applications for nanopore sequencing [PDF]

Nature Reviews Genetics, 2019
Two new studies report advances for nanopore-based long-read sequencing for characterizing complex human transcriptomes and for analysing human repetitive DNA regions.
openaire   +2 more sources

Wafer‐Scale Dendritic Nanoporous Platinum Films for Neural Electrodes

Advanced Science, EarlyView.
Wafer‐scale dendritic nanoporous platinum films with intrinsic nanoscale porosity are synthesized by suraface‐confined wet chemistry and transferred onto flexible substrates to create tissue‐conformal neural electrodes. The ultrathin films combine mechanical flexibility with electrochemical stability, enabling low‐impedance, high‐capacitance interfaces
Kyeongbin Kang, Joosung Oh, Soumen Dutta, Sun Woo Jang, Byeong Su Gu, Woosung Cho, Mingyu Kwak, Sunguk Hong, Seongjae Lee, Hyo Kyoung Jeon, Juwan Han, Keehoon Kim, Sung‐Min Park, Unyong Jeong, In Su Lee   +14 more
wiley   +1 more source

Primed and ready: nanopore metabarcoding can now recover highly accurate consensus barcodes that are generally indel-free

BMC Genomics
Background DNA metabarcoding applies high-throughput sequencing approaches to generate numerous DNA barcodes from mixed sample pools for mass species identification and community characterisation.
Jia Jin Marc Chang, Yin Cheong Aden Ip, Wan Lin Neo, Maxine A. D. Mowe, Zeehan Jaafar, Danwei Huang   +5 more
doaj   +1 more source

Nanopore sequencing technology in forensic science: status quo and tendency

Annals of Human Biology
Context Next-generation sequencing (NGS) is widely used in forensics for high throughput but limited by short reads and high non-batch testing costs. Third-generation sequencing (TGS), especially Oxford Nanopore Technologies (ONT) nanopore sequencing ...
Tianzhen Gao, Wang He, Xin Feng, Bing Long, Deming Zhang   +4 more
doaj   +1 more source