Results 151 to 160 of about 4,018 (199)

Optimizing rapeseed protein purification: a continuous chromatographic approach for napin and cruciferin

Journal of Chemical Technology & Biotechnology
Napin and cruciferin, the predominant storage proteins in Brassica napus (rapeseed), exhibit favorable techno‐functional properties such as emulsification and gel formation, positioning them as promising candidates for the encapsulation and delivery of ...
Jonas Arnecke, Jessica Gillmann, Tim Börner, M. Hafner, Christian Frech   +4 more
semanticscholar   +3 more sources

Recombinant napin expression in Pichia pastoris: Structural and functional attributes in comparison with canola-derived napin.

International Journal of Biological Macromolecules
The increasing demand for alternative proteins necessitates efficient production platforms. This study establishes a scalable method for producing recombinant napin (rNapin) using Pichia pastoris and compares it to canola-derived napin (cNapin).
Paz Shemesh, Hanfeng Cai, Raul-Ricardo Barzallo-Mora, A. Maršavelski, Ayelet Fishman   +4 more
semanticscholar   +2 more sources

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Improved in vitro digestibility of rapeseed napin proteins in mixtures with bovine beta-lactoglobulin.

Food Research International, 2019
Mixing of different protein sources can lead to either predictable, synergistic, or antagonistic effects on the protein digestibility. This study investigated the in vitro protein digestibility (IVPD) of protein mixtures between a napin-rich rapeseed ...
M. S. Joehnke, R. Lametsch, J. Sørensen
semanticscholar   +4 more sources

Thermoreversible Carrageenan Gels Formed Through Crosslinking with Napin

Food Hydrocolloids
Gireeshkumar Balakrishnan, Maria Moutkane, C. P. Mudau, Christophe Chassenieux, T. Nicolai   +4 more
semanticscholar   +2 more sources

pH and NaCl Dependent Complexation Between Rapeseed Cruciferin and Napin

Food Hydrocolloids
The interaction between the main protein components of rapeseed, also called canola, was investigated in water as a function of the pH and in the presence of NaCl. Firstly, the solubility of purified cruciferin and napin was determined and the soluble fraction was characterized using light scattering techniques.
C. P. Mudau, Maria Moutkane, C. Chassenieux, Marianne N. Lund, T. Nicolai   +4 more
semanticscholar   +3 more sources

Anti-Inflammatory and Transepithelial Transport Activities of Rapeseed (Brassica napus) Napin-Derived Dipeptide Thr-Leu in Caco-2 and RAW264.7 Cocultures.

Journal of Agricultural and Food Chemistry, 2023
This study aimed to investigate the anti-inflammatory molecular activity of rapeseed napin-derived dipeptide Thr-Leu (TL) using Caco-2/RAW264.7 cell cocultures.
Jing Zhang, Yijun Yao, Feiran Xu, Q. Yuan, Xingrong Ju, Lifeng Wang   +5 more
semanticscholar   +1 more source

A strategy for targeting recombinant proteins to protein storage vacuoles by fusion to Brassica napus napin in napin-depleted seeds.

Protein Expression and Purification, 2014
Seeds are capable of accumulating high levels of seed storage proteins (SSP), as well as heterologous proteins under certain conditions. Arabidopsis thaliana was used to develop a strategy to deplete seeds of an endogenous SSP and then replenish them with the same protein fused to a heterologous protein.
D. Hegedus, M. Barón, Natalie Labbé, C. Coutu, D. Lydiate, Helen Lui, K. Rozwadowski   +6 more
semanticscholar   +3 more sources

Structural and Functional Annotation of Napin-Like Protein from Momordica charantia to Explore its Medicinal Importance

Biochemical Genetics, 2021
Seed storage proteins not just provide essential nutritional ingredients for growth of seedlings but also have their potential role in defense mechanisms of plants. Napin is a seed storage protein and belongs to 2S albumin family. Napin and napin-like protein have many biological defensive activities including antifungal, antimicrobial, trypsin ...
Rawaba Arif, M. Zia, G. Mustafa
semanticscholar   +3 more sources

Assessment of Novel Oligopeptides from Rapeseed Napin (Brassica napus) in Protecting HepG2 Cells from Insulin Resistance and Oxidative Stress.

Journal of Agricultural and Food Chemistry, 2022
Oligopeptides (Thr-His-Leu-Pro-Lys (THLPK), His-Pro-Leu-Lys (HPLK), Leu-Pro-Lys (LPK), His-Leu-Lys (HLK), and Leu-His-Lys (LHK)) are newly identified from rapeseed napin (Brassica napus) protein-derived hydrolysates with the capability of upregulating ...
Meng Yao, Feiran Xu, Yijun Yao, Haiou Wang, Xingrong Ju, Lifeng Wang   +5 more
semanticscholar   +1 more source

Screening and identification of high bioavailable oligopeptides from rapeseed napin (Brassica napus) protein-derived hydrolysates via Caco-2/HepG2 co-culture model.

Food Research International, 2022
Rapeseed napin (Brassica napus) protein-derived hydrolysates (RNPHs, 1-4) are mixtures of peptides, prior to reaching liver tissue and playing their antidiabetic role, at least being absorbed and metabolized by the intestinal barrier.
Meng Yao, Yijun Yao, Bowen Qin, Mengmeng Pan, Xingrong Ju, Feiran Xu, Lifeng Wang   +6 more
semanticscholar   +1 more source