Results 111 to 120 of about 1,462,911 (314)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

LRR1 involved in the abscisic acid signaling pathway to regulate the early growth and development of Arabidopsis thaliana [PDF]

PeerJ
Background Living organisms possess the remarkable capacity to swiftly adapt to fluctuations in their environment. In the context of cell signal transduction, a significant challenge lies in ensuring the effective perception of external signals and the ...
Xiaoyang Xie, Lei Wei, Hongyuan Han, Bingnian Jing, Yuqing Liu, Yong Zhou, Ningjie Li, Xiao Li, Wei Wang   +8 more
doaj   +2 more sources

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

FEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Reduced Thioredoxin Regulates IL-1β Secretion via NLRP3 of IL-1β+ Alveolar Macrophages in COPD

Journal of Inflammation Research
Lueli Wang,1,* Rui Shi,1,* Zhaoliang Li,1 Ruoqiu Ma,1 Chongyu Wang,1,2 Changli Xu,1 Rong Guo,1 Chuang Xiao,1 Xiaohua Du,3 Weimin Yang1 1Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming ...
Wang L, Shi R, Li Z, Ma R, Wang C, Xu C, Guo R, Xiao C, Du X, Yang W   +9 more
doaj  

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Computational strategies to identify, prioritize and design potential antimalarial agents from natural products [PDF]

, 2015
Philosophiae Doctor - PhDIntroduction: There is an exigent need to develop novel antimalarial drugs in view of the mounting disease burden and emergent resistance to the presently used drugs against the malarial parasites.
Egieyeh, Samuel Ayodele
core   +1 more source

Effect of tobacco–radish rotation for different years on bacterial wilt and rhizosphere microbial communities

AMB Express
Tobacco bacterial wilt is a major limiting factor for tobacco production and development, and it is more likely to occur under perennial single cropping of tobacco. In recent years, the rotation of tobacco-radish has gradually become popular.
Yuhao Dai, Jixiu Li, Zhenzhen Wang, Shaoqi Yang, Qingju Xiao, Zipeng Gao, Fengjing Zhang, Chenran Zhao, Liang Yang, Shaopeng Chen, Wei Ding   +10 more
doaj   +1 more source

Database for Rapid Dereplication of Known Natural Products Using Data from MS and Fast NMR Experiments

, 2017
The discovery of novel and/or new bioactive natural products from biota sources is often confounded by the reisolation of known natural products. Dereplication strategies that involve the analysis of NMR and MS spectroscopic data to infer structural ...
Zani, Carlos L., Carroll, Anthony, Carlos L. Zani, Carroll, Anthony R., Anthony R. Carroll, Carroll, Anthony R, Zani, Carlos L, Zani, Carlos Leomar   +7 more
core   +1 more source

Strategies for Natural Products Discovery from Uncultured Microorganisms

, 2021
Microorganisms are highly regarded as a prominent source of natural products that have significant importance in many fields such as medicine, farming, environmental safety, and material production. Due to this, only tiny amounts of microorganisms can be
Khorshed Alam, Youming Zhang, Aiying Li, Muhammad Nazeer Abbasi, Jinfang Hao   +4 more
core   +1 more source